Itga6 (NM_001277970) Mouse Tagged ORF Clone

CAT#: MR231573

Itga6 (myc-DDK-tagged) - Mouse integrin alpha 6 (Itga6), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Itga6
• Synonyms: 5033401O05Rik; AI115430; Cd49f; VLA-6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231573 representing NM_001277970
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGTCGCGGGCCAGTTGTGCCTGCTCTACCTGTCCGCGGGGCTTCTAGCCCGGCTGGGTACAGCCT
  TCAACCTGGACACCCGCGAGGACAACGTGATCCGGAAATCGGGGGATCCCGGGAGCCTCTTCGGCTTCTC
  GCTCGCCATGCACTGGCAGTTGCAGCCGGAGGACAAGCGGCTGTTGCTTGTGGGGGCACCTCGGGCAGAA
  GCACTCCCGCTGCAGAGGGCGAACAGAACAGGGGGCCTGTACAGCTGTGACATCACCTCCCGGGGACCTT
  GTACACGGATTGAATTTGATAATGACGCTGATCCTATGTCAGAAAGCAAGGAAGACCAGTGGATGGGAGT
  CACTGTTCAGAGCCAAGGTCCAGGGGGCAAAGTGGTGACGTGTGCACATCGATATGAGAAACGGCAGCAC
  GTCAACACGAAGCAGGAGTCGCGGGATATCTTTGGAAGATGTTATGTCCTAAGTCAGAATCTCAGAATTG
  AAGATGATATGGACGGAGGAGACTGGAGTTTCTGCGATGGCCGGTTGAGAGGCCATGAAAAGTTTGGCTC
  CTGTCAGCAAGGAGTAGCGGCTACTTTCACTAAGGACTTTCATTACATTGTTTTTGGAGCCCCAGGGACT
  TACAACTGGAAAGGGATCGTCCGTGTAGAACAAAAGAATAACACTTTTTTTGACATGAACATCTTTGAAG
  ATGGGCCCTATGAAGTTGGTGGAGAGACTGATCATGATGAAAGTCTCGTGCCCGTTCCTGCTAACAGTTA
  CCTAGGCTTTTCACTGGACTCAGGGAAGGGTATTGTTTCTAAAGATGACATCACTTTTGTGTCTGGTGCT
  CCAAGAGCCAATCACAGTGGGGCTGTAGTTTTGCTAAAAAGAGACATGAAGTCCGCGCATCTGCTCCCTG
  AGTATATATTTGACGGAGAAGGCCTGGCTTCCTCGTTTGGCTATGATGTGGCAGTGGTGGACCTCAATGC
  AGATGGGTGGCAAGACATTGTTATCGGAGCTCCACAGTATTTTGATAGGGATGGTGAAGTCGGGGGTGCA
  GTTTACGTCTACATTAACCAGCAAGGCAAATGGAGTAATGTGAAGCCGATTCGTCTAAATGGGACCAAAG
  ACTCGATGTTTGGAATCTCTGTGAAAAATATAGGTGATATTAACCAAGATGGCTATCCAGATATTGCTGT
  TGGAGCTCCCTATGATGATCTGGGGAAGGTTTTTATCTATCATGGATCCCCGACTGGCATAATTACCAAG
  CCAACACAGGTTCTCGAGGGGACATCGCCTTACTTCGGCTATTCAATCGCTGGGAATATGGACCTGGATC
  GGAATTCCTACCCCGACCTTGCTGTGGGCTCCCTCTCAGACTCGGTCACTATTTTCAGATCCCGGCCAGT
  GATTAACATTCTAAAAACCATCACAGTGACTCCTAACAGAATTGACCTCCGCCAGAAGTCCATGTGTGGC
  TCACCTAGCGGGATATGCCTCAAGGTTAAAGCCTGTTTTGAATATACTGCGAAACCTTCCGGTTATAACC
  CTCCAATATCAATTTTGGGTATTCTCGAAGCTGAAAAAGAAAGAAGAAAGTCAGGGTTGTCATCGAGAGT
  TCAGTTTCGAAACCAAGGTTCCGAGCCAAAGTATACTCAGGAGCTGACCCTGAATCGGCAGAAGCAGCGG
  GCGTGCATGGAGGAGACCCTCTGGCTGCAGGAGAACATCAGAGACAAGCTGCGTCCCATCCCCATCACGG
  CTTCTGTGGAGATCCAGGAGCCCAGCTCTCGCCGGCGGGTGAACTCACTCCCCGAAGTTCTTCCCATCCT
  GAATTCAAATGAAGCCAAAACGGTCCAGACAGATGTCCACTTCTTAAAGGAAGGATGTGGAGACGACAAT
  GTCTGTAACAGCAACCTTAAGCTAGAGTATAAATTTGGTACCCGAGAAGGAAATCAAGACAAATTCTCTT
  ACCTTCCAATTCAAAAAGGCATCCCAGAATTAGTCCTAAAAGATCAGAAAGATATAGCTCTGGAAATAAC
  GGTGACCAACAGCCCTTCGGATCCAAGGAATCCCCGGAAAGATGGCGACGATGCCCATGAAGCCAAACTC
  ATCGCCACGTTTCCAGACACTCTGACATATTCCGCTTACAGAGAACTGAGGGCTTTCCCTGAGAAGCAGC
  TGAGCTGTGTGGCCAACCAGAATGGCTCCCAAGCCGACTGTGAGCTCGGAAATCCTTTCAAGAGAAATTC
  CAGTGTTACTTTCTATCTGATTTTAAGTACAACCGAGGTCACCTTTGACACCACAGATCTGGATATTAAT
  CTGAAGTTGGAAACAACAAGCAATCAGGATAATTTGGCTCCAATTACAGCGAAGGCAAAAGTGGTTATTG
  AATTGCTTTTATCGGTCTCCGGAGTCGCTAAGCCTTCGCAGGTGTATTTTGGAGGTACAGTTGTTGGTGA
  GCAAGCTATGAAATCTGAAGATGAAGTAGGAAGTTTAATAGAGTATGAATTTAGGGTGATTAACTTAGGC
  AAGCCTCTTAAAAACCTCGGCACAGCAACCTTGAATATACAGTGGCCCAAGGAGATTAGCAATGGCAAAT
  GGTTGCTTTATTTGATGAAAGTTGAATCCAAAGGTTTGGAGCAGATTGTTTGTGAGCCACACAATGAAAT
  AAACTACCTGAAGCTGAAGGAGTCTCACAACTCAAGAAAGAAACGGGAACTTCCTGAAAAACAGATAGAT
  GACAGCAGGAAATTTTCTTTATTTCCTGAAAGAAAATACCAGACTCTCAACTGCAGCGTCAACGTGAGGT
  GTGTGAACATCAGGTGCCCACTGCGAGGGCTGGACAGCAAGGCCTCTCTCGTTCTTCGTTCCAGGTTGTG
  GAACAGCACATTTCTAGAGGAATATTCCAAACTGAACTACTTGGACATTCTCCTGAGGGCTTCCATAGAT
  GTCACCGCTGCTGCTCAGAATATCAAGCTCCCTCACGCGGGCACTCAGGTTCGAGTGACGGTGTTTCCCT
  CAAAGACTGTAGCTCAGTATTCAGGAGTAGCTTGGTGGATCATCCTCCTGGCTGTTCTTGCCGGGATTCT
  GATGCTGGCTCTATTAGTGTTTTTACTGTGGAAGTGTGGATTCTTTAAGCGCTCTAGGTACGATGACAGC
  ATTCCCCGATACCATGCGGTGCGGATCCGGAAAGAAGAGCGAGAGATCAAAGATGAGAAACACATGGATA
  ACCTCGAAAAAAAACAGTGGATCACCAAGTGGAATGAAAACGAAAGTTACTCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231573 representing NM_001277970
  Red=Cloning site Green=Tags(s)
  
  MAVAGQLCLLYLSAGLLARLGTAFNLDTREDNVIRKSGDPGSLFGFSLAMHWQLQPEDKRLLLVGAPRAE
  ALPLQRANRTGGLYSCDITSRGPCTRIEFDNDADPMSESKEDQWMGVTVQSQGPGGKVVTCAHRYEKRQH
  VNTKQESRDIFGRCYVLSQNLRIEDDMDGGDWSFCDGRLRGHEKFGSCQQGVAATFTKDFHYIVFGAPGT
  YNWKGIVRVEQKNNTFFDMNIFEDGPYEVGGETDHDESLVPVPANSYLGFSLDSGKGIVSKDDITFVSGA
  PRANHSGAVVLLKRDMKSAHLLPEYIFDGEGLASSFGYDVAVVDLNADGWQDIVIGAPQYFDRDGEVGGA
  VYVYINQQGKWSNVKPIRLNGTKDSMFGISVKNIGDINQDGYPDIAVGAPYDDLGKVFIYHGSPTGIITK
  PTQVLEGTSPYFGYSIAGNMDLDRNSYPDLAVGSLSDSVTIFRSRPVINILKTITVTPNRIDLRQKSMCG
  SPSGICLKVKACFEYTAKPSGYNPPISILGILEAEKERRKSGLSSRVQFRNQGSEPKYTQELTLNRQKQR
  ACMEETLWLQENIRDKLRPIPITASVEIQEPSSRRRVNSLPEVLPILNSNEAKTVQTDVHFLKEGCGDDN
  VCNSNLKLEYKFGTREGNQDKFSYLPIQKGIPELVLKDQKDIALEITVTNSPSDPRNPRKDGDDAHEAKL
  IATFPDTLTYSAYRELRAFPEKQLSCVANQNGSQADCELGNPFKRNSSVTFYLILSTTEVTFDTTDLDIN
  LKLETTSNQDNLAPITAKAKVVIELLLSVSGVAKPSQVYFGGTVVGEQAMKSEDEVGSLIEYEFRVINLG
  KPLKNLGTATLNIQWPKEISNGKWLLYLMKVESKGLEQIVCEPHNEINYLKLKESHNSRKKRELPEKQID
  DSRKFSLFPERKYQTLNCSVNVRCVNIRCPLRGLDSKASLVLRSRLWNSTFLEEYSKLNYLDILLRASID
  VTAAAQNIKLPHAGTQVRVTVFPSKTVAQYSGVAWWIILLAVLAGILMLALLVFLLWKCGFFKRSRYDDS
  IPRYHAVRIRKEEREIKDEKHMDNLEKKQWITKWNENESYS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001277970
• ORF Size: 3273 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001277970.1, NP_001264899.1
• RefSeq Size: 5888
• RefSeq ORF: 3276
• Locus ID: 16403
• MW: 122.6 kDa
• Gene Summary: This gene encodes a protein that is a member of the integrin superfamily. Integrins are transmembrane receptors involved cell adhesion and signaling, and they are subdivided based on the heterodimer formation of alpha and beta chains. This protein has been shown to heterodimerize with beta 4 to bind laminin and to form the main component of hemidesmosomes, which mediate attachment of epithelia to basement membranes. In mouse, deficiency of this gene is associated with absence of hemidesmosomes, severe skin blistering, and early post-natal death. In humans mutations of this gene are associated with epidermolysis bullosa. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, May 2013]