Kcnma1 (NM_001253368) Mouse Tagged ORF Clone

CAT#: MR231689

Kcnma1 (myc-DDK-tagged) - Mouse potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (Kcnma1), transcript variant 12

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Kcnma1
• Synonyms: 5730414M22Rik; BKCa; MaxiK; mSlo; mSlo1; Slo; Slo1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231689 representing NM_001253368
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAAACGGTGGCGGCGGCGGCGGCGGCAGCAGCGGCGGCGGCGGCGGCGGCGGCGGAGGCAGCGGTC
  TTAGAATGAGCAGCAATATCCACGCGAACCATCTCAGCCTAGACGCGTCCTCCTCCTCCTCTTCCTCCTC
  CTCTTCTTCTTCTTCCTCCTCCTCTTCCTCCTCGTCCTCGGTCCACGAGCCCAAGATGGATGCGCTCATC
  ATACCGGTGACCATGGAGGTGCCGTGCGACAGCCGGGGCCAACGCATGTGGTGGGCTTTCTTGGCCTCCT
  CCATGGTGACTTTCTTCGGGGGCCTCTTCATCATCTTGCTCTGGCGGACGCTCAAGTACCTGTGGACCGT
  TTGCTGCCACTGCGGGGGCAAGACGAAGGAGGCCCAGAAGATAAACAATGGCTCCAGCCAGGCAGATGGT
  ACTCTCAAGCCAGTGGACGAAAAAGAGGAGGTGGTGGCAGCCGAGGTCGGCTGGATGACATCTGTGAAGG
  ACTGGGCAGGGGTGATGATATCCGCCCAGACACTGACTGGCAGAGTCCTGGTTGTGTTAGTCTTTGCTCT
  CAGCATTGGTGCCCTCGTAATATACTTCATAGACTCGTCAAACCCAATAGAATCCTGCCAGAATTTCTAC
  AAAGATTTCACATTACAGATCGACATGGCTTTCAACGTGTTCTTCCTCCTCTACTTTGGCTTGCGGTTTA
  TTGCAGCCAACGATAAGCTGTGGTTCTGGCTGGAAGTGAATTCAGTAGTAGATTTCTTCACAGTCCCTCC
  TGTGTTTGTGTCTGTGTACTTAAACAGAAGTTGGCTTGGCTTGAGATTTTTAAGAGCTCTCAGACTGATA
  CAGTTTTCAGAGATTTTGCAGTTTCTGAATATCCTTAAAACAAGTAACTCCATCAAGCTGGTGAATCTGC
  TCTCCATATTTATCAGCACGTGGCTGACTGCAGCTGGATTCATCCACTTGGTGGAGAATTCAGGGGACCC
  ATGGGAAAATTTCCAAAACAACCAGGCACTTACGTACTGGGAATGTGTCTACTTACTCATGGTCACAATG
  TCTACAGTGGGTTATGGGGACGTTTATGCAAAAACCACACTTGGACGCCTCTTCATGGTCTTCTTCATCC
  TCGGGGGACTGGCCATGTTTGCCAGCTACGTCCCTGAAATCATAGAGTTAATAGGAAACCGCAAGAAATA
  CGGGGGCTCCTATAGCGCGGTTAGTGGAAGAAAGCACATTGTAGTCTGTGGACACATTACTCTGGAGAGT
  GTCTCTAACTTCCTGAAGGACTTTCTGCACAAGGACCGGGATGATGTCAACGTGGAGATTGTCTTTCTTC
  ACAACATCTCCCCTAACCTTGAGCTTGAGGCTCTGTTCAAACGGCATTTCACTCAGGTGGAGTTTTATCA
  GGGCTCTGTCCTCAATCCACATGATCTTGCCAGAGTCAAGATAGAGTCAGCAGATGCATGCCTGATCCTT
  GCCAATAAGTATTGCGCTGACCCGGATGCAGAAGATGCCTCCAACATCATGAGAGTGATCTCCATCAAAA
  ACTACCACCCAAAGATCAGGATCATCACTCAGATGCTGCAGTATCACAACAAGGCCCATCTGCTCAACAT
  CCCCAGCTGGAACTGGAAAGAGGGTGATGACGCAATATGCCTTGCAGAGCTCAAGTTGGGTTTCATAGCC
  CAGAGCTGTCTGGCTCAAGGCCTCTCCACAATGCTTGCCAATCTCTTCTCTATGAGGTCATTCATAAAGA
  TTGAGGAAGACACATGGCAGAAATACTACTTGGAAGGAGTCTCCAATGAAATGTACACAGAATATCTCTC
  CAGTGCCTTCGTGGGTCTGTCCTTCCCTACTGTTTGTGAGCTGTGTTTTGTGAAGCTTAAGCTCCTGATG
  ATAGCCATTGAGTACAAGTCTGCCAACAGAGAGAGCCGAAGCCGAAAGCGAATATTAATTAACCCTGGGA
  ACCACCTTAAGATCCAAGAAGGTACTTTAGGATTTTTCATCGCAAGTGATGCCAAAGAAGTTAAAAGGGC
  ATTTTTTTACTGCAAGGCCTGTCATGATGACGTCACAGATCCCAAAAGAATTAAAAAATGTGGCTGCAGG
  CGGCTCAAGGTTGAAGCTAGAGCCCGCTATCACAAAGACCCATTTATGCACAAGAATGCGACTCCCAATT
  CTCCACACGTGCCCAAGCCAGTTGAAGATGAGCAGCCGCCAACCCTGTCACCAAAAAAAAAACAACGTAA
  TGGGGGCATGAGGAACTCGCCCAACACCTCCCCGAAGCTGATGAGGCATGACCCCTTGTTAATTCCTGGC
  AATGATCAGATTGACAACATGGACTCCAATGTGAAAAAGTACGACTCCACTGGAATGTTTCACTGGTGTG
  CACCCAAGGAGATTGAGAAAGTCATCTTGACTCGAAGTGAAGCTGCCATGACTGTCCTGAGTGGCCATGT
  CGTAGTCTGCATCTTTGGGGATGTCAGCTCAGCCCTGATTGGCCTCCGGAACCTGGTGATGCCACTTCGT
  GCTAGCAACTTTCACTATCATGAGCTCAAACACATTGTGTTTGTGGGCTCCATTGAGTACCTCAAGAGGG
  AGTGGGAAACACTGCACAACTTCCCGAAAGTGTCCATATTGCCTGGTACACCATTAAGTCGGGCTGATTT
  AAGGGCTGTCAACATCAACCTCTGTGACATGTGCGTTATCCTGTCAGCCAATCAGAATAATATTGATGAT
  ACTTCGCTTCAGGACAAGGAATGCATCTTGGCGTCACTCAACATCAAATCTATGCAGTTTGATGACAGCA
  TCGGGGTCTTGCAGGCTAATTCCCAAGGATTCACACCTCCTGGAATGGACAGATCATCACCCGACAACAG
  CCCAGTGCACGGGATGTTACGCCAGCCGTCCATCACAACTGGGGTCAACATTCCCATCATCACGGAACTC
  GTGAATGATACCAATGTTCAGTTTTTGGACCAAGACGATGACGATGACCCTGACACAGAGCTGTACCTCA
  CACAGCCCTTTGCTTGTGGGACAGCATTTGCCGTCAGCGTCCTGGACTCACTCATGAGCGCGACATACTT
  CAATGACAATATCCTCACCCTAATACGGACCCTGGTGACAGGAGGAGCCACACCAGAGCTCGAGGCTCTA
  ATAGCTGAGGAGAATGCACTTCGAGGAGGCTACAGCACTCCGCAGACATTGGCCAACAGGGACCGTTGCC
  GAGTGGCCCAGTTAGCCCTGTTAGATGGTCCCTTTGCAGACTTAGGGGATGGTGGTTGTTATGGTGATCT
  GTTCTGCAAAGCTCTGAAAACATATAATATGCTTTGTTTTGGAATTTACCGGCTGAGAGATGCCCACCTC
  AGCACCCCCAGCCAGTGTACAAAAAGGTACGTCATCACCAATCCTCCCTACGAGTTTGAGCTCGTACCAA
  CAGACCTGATCTTCTGCCTGATGCAGTTTGACCACAACGCTGGCCAATCCCGGGCCAGTCTGTCTCATTC
  CTCCCACTCCTCACAGTCGTCCAGTAAGAAGAGCTCCTCCGTCCACTCCATCCCGTCCACAGCAAATCGG
  CCGAACCGGCCCAAGTCCAGGGAGTCCCGCGACAAACAGAAGTACGTTCAGGAAGAGCGGCTT
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231689 representing NM_001253368
  Red=Cloning site Green=Tags(s)
  
  MANGGGGGGGSSGGGGGGGGGSGLRMSSNIHANHLSLDASSSSSSSSSSSSSSSSSSSSSVHEPKMDALI
  IPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGGKTKEAQKINNGSSQADG
  TLKPVDEKEEVVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFY
  KDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLI
  QFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWENFQNNQALTYWECVYLLMVTM
  STVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLES
  VSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLIL
  ANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIA
  QSCLAQGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLSFPTVCELCFVKLKLLM
  IAIEYKSANRESRSRKRILINPGNHLKIQEGTLGFFIASDAKEVKRAFFYCKACHDDVTDPKRIKKCGCR
  RLKVEARARYHKDPFMHKNATPNSPHVPKPVEDEQPPTLSPKKKQRNGGMRNSPNTSPKLMRHDPLLIPG
  NDQIDNMDSNVKKYDSTGMFHWCAPKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLR
  ASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDD
  TSLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGMLRQPSITTGVNIPIITEL
  VNDTNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEAL
  IAEENALRGGYSTPQTLANRDRCRVAQLALLDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHL
  STPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSQSSSKKSSSVHSIPSTANR
  PNRPKSRESRDKQKYVQEERL
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001253368
• ORF Size: 3633 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001253368.1, NP_001240297.1
• RefSeq Size: 6119
• RefSeq ORF: 3636
• Locus ID: 16531
• MW: 135.3 kDa