Plcb3 (NM_001290349) Mouse Tagged ORF Clone

CAT#: MR231706

Plcb3 (myc-DDK-tagged) - Mouse phospholipase C, beta 3 (Plcb3), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Plcb3
• Synonyms: mKIAA4098
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231706 representing NM_001290349
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGGCGCGAGGCCCGGCGTCCACGCGCTGCAGCTGGAGCCGCCCACCGTGGTGGAGACCCTGCGGC
  GCGGGAGTAAGTTCATCAAATGGGACGAGGAGGCCTCCAGTCGGAACCTGGTGACCCTGCGTGTGGACCC
  TAATGGCTTCTTCTTGTACTGGACAGGACCCAACATGGAGGTGGACACACTGGACATCAGCTCCATCAGG
  GACACAAGGACAGGTCGTTATGCCCGCCTGCCCAAGGACCCTAAGATTCGAGAAGTACTGGGCTTTGGAG
  GTCCTGACACCCGGCTGGAGGAGAAACTGATGACAGTGGTGGCCGGGCCAGATCCAGTAAATACCACATT
  CTTGAACTTCATGGCCGTGCAAGATGACACAGTCAAGGTCTGGTCAGAGGAGTTGTTTAAACTGGCCATG
  AACATATTGGCTCAGAACGCCTCCCGGAACACCTTCCTGCGGAAAGCATACACGAAGCTGAAGCTGCAGG
  TGAACCAGGATGGACGGATTCCGGTCAAGAACATTCTGAAGATGTTCTCGGCGGACAAGAAGCGGGTGGA
  GACGGCGCTGGAGTCCTGTGGCCTCAACTTCAACCGAAGTGAGTCCATTCGCCCTGATGAGTTTCCCTTG
  GAAATTTTTGAGCGGTTCTTGAATAAACTGTGTCTGCGCCCGGATATTGACAAGATCCTGCTGGAGATAG
  GTGCCAAGGGCAAGCCGTACCTCACTCTGGAGCAGCTCATGGACTTTATCAACCAGAAGCAGAGAGACCC
  GAGACTCAACGAAGTGCTGTACCCGCCACTTCGGTCCTCACAGGCTCGGCTGCTCATTGAGAAGTACGAG
  ACGAACAAACAGTTCCTGGAGCGCGACCAGATGTCTATGGAGGGCTTCAGCCGCTACCTGGGAGGGGAGG
  AGAATGGTATCCTGCCTCTGGAGGCCCTGGATCTGAGCATGGACATGACCCAGCCACTGAGCGCATATTT
  TATCAACTCCTCACACAACACCTATCTCACTGCGGGCCAGCTGGCTGGACCATCATCGGTGGAGATGTAC
  CGCCAGGCACTGCTGTGGGGCTGCCGCTGTGTGGAGCTGGATGTATGGAAGGGACGGCCACCGGAGGAAG
  AGCCTTTCATCACTCATGGTTTCACCATGACCACTGAGGTGCCATTGCGTGATGTGCTAGAGGCCATCGC
  TGAGGCAGCCTTCAAGACCTCGCCCTACCCTGTCATCCTCTCCTTTGAGAACCATGTCGACTCGGCAAAG
  CAGCAGGCCAAGATGGCTGAGTACTGCCGCTCTATCTTTGGGGATGCGCTGCTCATTGACCCTCTCGACA
  AATACCCGCTATCTGCGGGCATCCCCCTGCCTAGTCCACAGGACCTGATGGGCCGTATCCTGGTGAAGAA
  TAAGAAGCGACATCGGCCCAGCACAGGTGTCCCTGACAGCTCAGTGCGCAAGCGGCCTCTGGAACAGAGC
  AACTCGGCCTTGAGTGAGAGCTCGGCCGCCACAGAGCCCTCCTCACCTCAGCTTGGGTCCCCCAGCTCCG
  ACAGCTGCCCTGGCCTAAGCAATGGGGAGGAGGTGGGACTCGAGAAGACCAGCCTGGAGCCTCAGAAGTC
  TCTAGGTGAGGAGAGCCTCAGCCGGGAACCTAATGTGCCCATGCCTGACCGTGACCGAGAGGATGAGGAG
  GAAGATGAAGAAGAAGAGGAAACAACGGATCCAAAAAAGCCCACCACTGATGAGGGCACAGCCAGCAGTG
  AGGTCAATGCCACGGAGGAGATGTCAACGCTCGTCAACTACGTTGAGCCCGTCAAGTTCAAGTCCTTCGA
  GGCTGCTCGAAAAAGGAACAAGTGCTTCGAGATGTCATCCTTCGTGGAGACCAAGGCAATGGAGCAACTG
  ACCAAGAGTCCTATGGAGTTTGTGGAATACAACAAACAGCAGCTCAGCCGCATCTACCCTAAGGGCACAC
  GAGTGGACTCCTCCAACTACATGCCTCAGCTCTTCTGGAACGTGGGTTGCCAGCTTGTTGCCCTCAACTT
  CCAGACCTTGGATTTGCCAATGCAGCTCAACGCAGGCGTGTTTGAGTACAATGGGCGCAGCGGGTACTTG
  CTCAAGCCCGAGTTTATGCGGCGGCCGGACAAGTCTTTTGATCCCTTCACTGAGGTCATCGTAGATGGCA
  TAGTGGCCAACGCCTTGCGGGTCAAGGTGATTTCGGGGCAGTTCCTGTCTGACAAGAAGGTGGGCATCTA
  CGTCGAGGTGGACATGTTTGGCCTCCCAGTTGACACAAGACGCAAATATCGTACCCGGACATCCCAGGGG
  AACTCATTCAACCCTGTGTGGGACGAGGAACCCTTTGACTTCCCCAAGGTTGTGCTGCCCACGCTGGCCT
  CACTTCGCATCGCAGCCTTTGAGGAGGGTGGCAAATTTGTTGGGCACCGTATCCTGCCTGTCTCTGCTAT
  CCGCTCAGGATACCACTATGTCTGCCTGCGAAACGAGGCCAACCAACCTTTGTGCCTGCCTGCCCTGCTT
  ATCTACACTGAAGCTTCTGACTACATCCCAGATGACCACCAGGACTATGCGGAGGCCTTGATTAACCCCA
  TCAAGCACGTAAGCCTAATGGACCAGCGGGCCAAGCAACTAGCCGCTCTCATTGGGGAGAGTGAGGCTCA
  GGCCAGCACAGAGACATATCAGGAGACCCCGTGTCAACAGCCAGGGTCACAGCTCCCCTCCAACCCCACA
  CCTAACCCACTGGATGCCTCACCTCGCTGGCCCCCCGGTCCTACCACTTCCTCCACTAGCTCCTCCCTCA
  GTAGCCCAGGGCAGCGAGATGATTTGATTGCCAGCATCCTCTCTGAGGTGACCCCTACACCTCTAGAGGA
  GCTCAGAAGCCACAAGGCTATGGTGAAGCTACGGAGCCGGCAGGATCGAGACCTGCGGGAGTTGCATAAG
  AAGCACCAGCGCAAGGCTGTGGCCCTCACCCGCCGCCTTCTGGATGGCCTGGCCCAGGCCAGGGCTGAGG
  GGAAGTGCCGGCCCTCCCCCAGTGCCCTGGGCAAGGCCACCAACTCGGAGGACGTGAAGGAGGAAGAGGA
  GGCCAAACAGTATCGAGAGTTCCAGAACCGACAAGTACAGAGCCTGTTGGAGCTGAGGGAAGCCCAGGCA
  GATGTGGAGACCAAGCGGAAGCTGGAGCATCTACGACAGGCTCATCAGCGGCTCAAGGAGGTTGTCCTGG
  ATACACACACAACACAGTTCAAGAGGCTGAAGGAGCTGAATGAAAGGGAGAAGAAGGAACTCCAGAAGAT
  CCTGGACAGGAAGCGCAACAACAGCATCTCAGAGGCCAAGACAAGGGAGAAACACAAGAAGGAGGTGGAA
  CTGACAGAGATTAATCGGCGGCACATCACTGAGTCGGTAAACTCCATCAGACGGCTGGAAGAGGCCCAGA
  AGCAGCGCCATGAACGCCTGGTGGCAGGGCAACAGCAAGTCCTCCAGCAGCTAGAGGAAGAGGAACCCAA
  GCTGCTGGCCCAGCTGACCCAGGAGTGTCAGGAACAGCGAGAGAGGTTGCCCCAGGAGATCCGTCGGTGC
  CTGCTGGGCGAGACAGCAGAGGGACTGGGGGACGGCCCCCTGGTGGCCTGTGCCAGCAATGGTCATGCAC
  CTGGGAGTGGTGGGCACCTGTCCAGCGCTGACTCGGAGAGCCAGGAGGAGAACACCCAGCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231706 representing NM_001290349
  Red=Cloning site Green=Tags(s)
  
  MAGARPGVHALQLEPPTVVETLRRGSKFIKWDEEASSRNLVTLRVDPNGFFLYWTGPNMEVDTLDISSIR
  DTRTGRYARLPKDPKIREVLGFGGPDTRLEEKLMTVVAGPDPVNTTFLNFMAVQDDTVKVWSEELFKLAM
  NILAQNASRNTFLRKAYTKLKLQVNQDGRIPVKNILKMFSADKKRVETALESCGLNFNRSESIRPDEFPL
  EIFERFLNKLCLRPDIDKILLEIGAKGKPYLTLEQLMDFINQKQRDPRLNEVLYPPLRSSQARLLIEKYE
  TNKQFLERDQMSMEGFSRYLGGEENGILPLEALDLSMDMTQPLSAYFINSSHNTYLTAGQLAGPSSVEMY
  RQALLWGCRCVELDVWKGRPPEEEPFITHGFTMTTEVPLRDVLEAIAEAAFKTSPYPVILSFENHVDSAK
  QQAKMAEYCRSIFGDALLIDPLDKYPLSAGIPLPSPQDLMGRILVKNKKRHRPSTGVPDSSVRKRPLEQS
  NSALSESSAATEPSSPQLGSPSSDSCPGLSNGEEVGLEKTSLEPQKSLGEESLSREPNVPMPDRDREDEE
  EDEEEEETTDPKKPTTDEGTASSEVNATEEMSTLVNYVEPVKFKSFEAARKRNKCFEMSSFVETKAMEQL
  TKSPMEFVEYNKQQLSRIYPKGTRVDSSNYMPQLFWNVGCQLVALNFQTLDLPMQLNAGVFEYNGRSGYL
  LKPEFMRRPDKSFDPFTEVIVDGIVANALRVKVISGQFLSDKKVGIYVEVDMFGLPVDTRRKYRTRTSQG
  NSFNPVWDEEPFDFPKVVLPTLASLRIAAFEEGGKFVGHRILPVSAIRSGYHYVCLRNEANQPLCLPALL
  IYTEASDYIPDDHQDYAEALINPIKHVSLMDQRAKQLAALIGESEAQASTETYQETPCQQPGSQLPSNPT
  PNPLDASPRWPPGPTTSSTSSSLSSPGQRDDLIASILSEVTPTPLEELRSHKAMVKLRSRQDRDLRELHK
  KHQRKAVALTRRLLDGLAQARAEGKCRPSPSALGKATNSEDVKEEEEAKQYREFQNRQVQSLLELREAQA
  DVETKRKLEHLRQAHQRLKEVVLDTHTTQFKRLKELNEREKKELQKILDRKRNNSISEAKTREKHKKEVE
  LTEINRRHITESVNSIRRLEEAQKQRHERLVAGQQQVLQQLEEEEPKLLAQLTQECQEQRERLPQEIRRC
  LLGETAEGLGDGPLVACASNGHAPGSGGHLSSADSESQEENTQL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001290349
• ORF Size: 3702 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001290349.1, NP_001277278.1
• RefSeq Size: 4273
• RefSeq ORF: 3705
• Locus ID: 18797
• MW: 139.9 kDa
• Gene Summary: The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. [UniProtKB/Swiss-Prot Function]