Apaf1 (NM_001282947) Mouse Tagged ORF Clone

CAT#: MR231716

Apaf1 (myc-DDK-tagged) - Mouse apoptotic peptidase activating factor 1 (Apaf1), transcript variant 3

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Apaf1
• Synonyms: 6230400I06Rik; Apaf-1; Apaf1l; fog; mKIAA0413
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231716 representing NM_001282947
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGCAAAGGCCCGCAATTGTTTGCTTCAACATAGAGAAGCTTTGGAAAAGGACATCAAAACATCCT
  ACATCATGGATCACATGATCAGTAATGGCGTCTTGTCAGTGATAGAGGAGGAGAAGGTCAAAAGTCAGGC
  CACTCAATATCAACGAGCAGCCGCTTTAATTAAAATGATACTTAATAAAGACAACTGTGCCTACATTTCA
  TTCTACAACGCTCTGCTACACGAGGGCTATAAGGACCTTGCTGCGCTTCTGCAGAGTGGCCTTCCTCTTG
  TGTCGTCTTCCAGTGTAAGGACAGTGCTGTGTGAAGGTGGAGTACCCCAGAGGCCGGTTATTTTCGTTAC
  TAGAAAGAAGCTGGTTCATGCGATTCAGCAGAAGCTCTGGAAACTGAATGGAGAACCAGGGTGGGTCACC
  ATCTATGGGATGGCAGGCTGCGGCAAGTCTGTGTTAGCTGCGGAAGCCGTTCGAGATCACTCCCTCTTAG
  AAGGTTGCTTTTCAGGGGGTGTACACTGGGTTTCCATTGGAAAACAAGACAAATCTGGGCTTCTCATGAA
  ACTGCAGAATCTGTGCATGCGCTTGGACCAAGAAGAGAGTTTCTCTCAGAGGCTTCCACTTAATATTGAG
  GAGGCCAAAGACCGCCTCCGTGTTCTGATGCTGCGCAAACACCCAAGGTCTCTGTTGATCTTGGATGATG
  TTTGGGATCCTTGGGTGTTAAAAGCTTTTGACAATCAGTGTCAGATTCTTCTTACAACCAGAGATAAGAG
  TGTTACAGATTCAGTAATGGGTCCTAAGCATGTTGTCCCTGTGGAGAGTGGTCTAGGGAGAGAGAAAGGA
  CTTGAGATCTTGTCACTTTTTGTTAACATGAAGAAAGAAGATCTGCCAGCGGAGGCTCACAGTATTATAA
  AGGAATGCAAAGGTTCTCCTCTTGTAGTGTCTTTAATTGGTGCGCTTTTACGTGATTTTCCCAATCGCTG
  GGCATACTACCTCAGACAGCTTCAGAATAAGCAGTTTAAGAGAATAAGGAAGTCTTCATCTTATGATTAT
  GAGGCTCTAGATGAAGCCATGTCGATAAGTGTTGAAATGCTCAGAGAAGACATCAAAGACTATTACACAG
  ACCTTTCCATCCTTCAGAAGGACGTCAAGGTACCTACAAAGGTGTTGTGCGTTCTCTGGGACTTGGAAAC
  GGAAGAAGTTGAAGACATCCTGCAGGAGTTCGTTAATAAGTCTCTCTTATTCTGTAATCGGAATGGAAAG
  TCATTTTGTTATTATTTACATGATCTTCAAGTAGATTTTCTTACAGAGAAGAATCGCAGTCAGCTTCAGG
  ATCTGCACAGGAAGATGGTCACTCAGTTTCAGAGGTATTACCAGCCCCACACGCTGTCTCCAGACCAGGA
  GGACTGCATGTATTGGTACAACTTCCTAGCCTATCACATGGCTAGTGCCAATATGCACAAAGAACTTTGT
  GCTTTAATGTTTTCCCTGGACTGGATTAAAGCAAAAACAGAACTTGTCGGCCCTGCCCATCTGATTCACG
  AGTTCGTGGCATATAGGCATATATTGGATGAAAAGGATTGTGCAGTCTGTGAGAATTTTCAAGAGTTTTT
  ATCTTTAAATGGACACCTCCTTGGACGACAGCCATTTCCTAATATTGTACAGCTGGGCCTCTGTGAACCA
  GAAACTTCCGAAGTTTATCGACAAGCAAAGCTGCAGGCCAAGCAGGAGGGGGATACTGGGCGCCTTTACC
  TGGAATGGATAAACAAAAAAACTATCAAGAATCTGTCCCGCTTAGTCGTCCGCCCCCACACAGATGCTGT
  TTACCACGCGTGTTTTTCTCAGGATGGTCAGAGAATAGCTTCTTGTGGGGCTGATAAAACCTTACAGGTG
  TTCAAAGCCGAGACAGGAGAGAAACTTCTTGACATCAAAGCTCACGAAGATGAGGTGCTTTGCTGCGCGT
  TCTCCTCAGACGACAGTTACATAGCGACCTGCTCAGCGGATAAGAAGGTTAAGATTTGGGATTCTGCGAC
  TGGGAAGCTTGTGCACACCTACGACGAGCACTCGGAGCAAGTCAATTGCTGCCATTTCACCAACAAGAGT
  AACCACCTTCTCTTGGCCACTGGGTCAAATGATTTCTTCCTCAAGCTCTGGGATTTGAATCAAAAAGAAT
  GTCGAAATACCATGTTTGGTCACACGAACTCAGTCAACCACTGCAGGTTCTCACCAGACGATGAGCTCTT
  GGCTAGCTGCTCAGCTGACGGGACTTTAAGGCTTTGGGATGTGAGATCAGCAAACGAGAGGAAAAGCATT
  AATGTGAAGCGCTTCTTCCTGAGTTCAGAAGACCCTCCAGAGGATGTGGAGGTGATCGTGAAGTGTTGTT
  CCTGGTCTGCAGATGGTGACAAAATAATAGTGGCAGCAAAAAACAAAGTCCTCCTTTTTGATATTCATAC
  TAGTGGCCTATTGGCAGAGATCCACACAGGCCATCACAGCACCATCCAGTACTGTGACTTCTCCCCCTAT
  GACCATTTGGCTGTGATTGCCCTGTCTCAGTACTGTGTGGAGTTGTGGAACATAGACTCCCGCCTAAAGG
  TGGCCGACTGCAGAGGACATTTGAGTTGGGTTCACGGTGTGATGTTTTCTCCCGATGGCTCCTCATTTTT
  GACAGCTTCTGATGACCAAACAATAAGGGTCTGGGAGACAAAAAAGGTATGCAAGAACTCTGCCATCGTG
  CTAAAGCAGGAAATAGACGTCGTGTTTCAAGAGAACGAAACGATGGTCCTTGCAGTTGACAACATAAGAG
  GCCTGCAACTCATTGCTGGAAAAACAGGCCAGATTGATTACCTGCCTGAAGCCCAAGTGAGTTGCTGCTG
  CCTCAGTCCACACCTTGAGTACGTGGCATTCGGAGATGAAGATGGAGCCATTAAGATTATAGAACTTCCA
  AACAACAGAGTCTTCAGTTCTGGGGTTGGGCACAAGAAAGCTGTGCGGCACATCCAGTTCACAGCTGATG
  GGAAGACACTGATTTCAAGTTCTGAAGATTCTGTGATTCAGGTATGGAATTGGCAGACAGGGGACTATGT
  ATTTTTGCAAGCCCACCAGGAAACGGTAAAGGACTTCAGGCTCCTCCAAGATTCAAGATTGCTTTCTTGG
  TCATTTGATGGAACGGTGAAGGTGTGGAATGTCATTACCGGAAGAATAGAAAGAGACTTTACTTGTCATC
  AGGGCACAGTGCTTTCCTGTGCTATCTCTTCTGATGCGACCAAGTTTTCATCTACCTCTGCTGATAAGAC
  TGCCAAGATCTGGAGTTTTGACCTCCTTTCCCCTCTTCATGAGCTGAAGGGCCATAATGGCTGTGTCCGC
  TGCTCTGCCTTCTCGCTGGATGGCATCCTGTTGGCAACTGGAGATGACAATGGAGAAATCCGGATATGGA
  ATGTCTCAGATGGCCAGCTTCTTCATTCGTGTGCTCCGATCTCGGTAGAGGAAGGAACTGCTACCCACGG
  CGGCTGGGTAACTGATGTGTGCTTCTCTCCCGACAGTAAAACGCTTGTCTCTGCTGGAGGATATCTCAAG
  TGGTGGAATGTTGCCACTGGGGACTCCTCACAGACCTTCTACACAAATGGAACAAACCTCAAGAAAATCC
  ACGTGTCCCCTGACTTCAGAACCTATGTGACTGTCGATAATCTCGGTATTTTATATATTTTACAGGTTTT
  AGAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231716 representing NM_001282947
  Red=Cloning site Green=Tags(s)
  
  MDAKARNCLLQHREALEKDIKTSYIMDHMISNGVLSVIEEEKVKSQATQYQRAAALIKMILNKDNCAYIS
  FYNALLHEGYKDLAALLQSGLPLVSSSSVRTVLCEGGVPQRPVIFVTRKKLVHAIQQKLWKLNGEPGWVT
  IYGMAGCGKSVLAAEAVRDHSLLEGCFSGGVHWVSIGKQDKSGLLMKLQNLCMRLDQEESFSQRLPLNIE
  EAKDRLRVLMLRKHPRSLLILDDVWDPWVLKAFDNQCQILLTTRDKSVTDSVMGPKHVVPVESGLGREKG
  LEILSLFVNMKKEDLPAEAHSIIKECKGSPLVVSLIGALLRDFPNRWAYYLRQLQNKQFKRIRKSSSYDY
  EALDEAMSISVEMLREDIKDYYTDLSILQKDVKVPTKVLCVLWDLETEEVEDILQEFVNKSLLFCNRNGK
  SFCYYLHDLQVDFLTEKNRSQLQDLHRKMVTQFQRYYQPHTLSPDQEDCMYWYNFLAYHMASANMHKELC
  ALMFSLDWIKAKTELVGPAHLIHEFVAYRHILDEKDCAVCENFQEFLSLNGHLLGRQPFPNIVQLGLCEP
  ETSEVYRQAKLQAKQEGDTGRLYLEWINKKTIKNLSRLVVRPHTDAVYHACFSQDGQRIASCGADKTLQV
  FKAETGEKLLDIKAHEDEVLCCAFSSDDSYIATCSADKKVKIWDSATGKLVHTYDEHSEQVNCCHFTNKS
  NHLLLATGSNDFFLKLWDLNQKECRNTMFGHTNSVNHCRFSPDDELLASCSADGTLRLWDVRSANERKSI
  NVKRFFLSSEDPPEDVEVIVKCCSWSADGDKIIVAAKNKVLLFDIHTSGLLAEIHTGHHSTIQYCDFSPY
  DHLAVIALSQYCVELWNIDSRLKVADCRGHLSWVHGVMFSPDGSSFLTASDDQTIRVWETKKVCKNSAIV
  LKQEIDVVFQENETMVLAVDNIRGLQLIAGKTGQIDYLPEAQVSCCCLSPHLEYVAFGDEDGAIKIIELP
  NNRVFSSGVGHKKAVRHIQFTADGKTLISSSEDSVIQVWNWQTGDYVFLQAHQETVKDFRLLQDSRLLSW
  SFDGTVKVWNVITGRIERDFTCHQGTVLSCAISSDATKFSSTSADKTAKIWSFDLLSPLHELKGHNGCVR
  CSAFSLDGILLATGDDNGEIRIWNVSDGQLLHSCAPISVEEGTATHGGWVTDVCFSPDSKTLVSAGGYLK
  WWNVATGDSSQTFYTNGTNLKKIHVSPDFRTYVTVDNLGILYILQVLE
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001282947
• ORF Size: 3714 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001282947.1, NP_001269876.1
• RefSeq Size: 6497
• RefSeq ORF: 3717
• Locus ID: 11783
• MW: 140.4 kDa