Flii (NM_001302207) Mouse Tagged ORF Clone

CAT#: MR231745

Flii (myc-DDK-tagged) - Mouse flightless I homolog (Drosophila) (Flii), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Flii
• Synonyms: 3632430F08Rik; Fliih
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231745 representing NM_001302207
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCCACCGGGGTGCTGCCGTTCGTGCGCGGCGTGGACCTCAGCGGCAACGACTTCAAGGGTGGCT
  ACTTCCCTGAGAATGTTAAGGCTATGACCAGCCTGCGATGGCTAAAGCTGAATCGCACAGGCCTCTGCTA
  CCTGCCTGAAGAACTTGCGGCTCTGCAGAAGCTGGAGCACCTGTCTGTGAGCCACAACCATCTGACCACG
  CTTCACGGCGAACTGTCTAGCCTGCCTTCGCTTCGGGCCATTGTAGCTCGGGCCAACAGCCTGAAGAATT
  CCGGAGTCCCTGATGACATCTTCAAGCTGGATGATCTCTCTGTTCTGGACTTGAGCCATAACCAGTTGAC
  AGAATGCCCACGGGAGCTGGAGAATGCCAAGAACATGCTGGTGCTCAACCTCAGCCACAACGGCATCGAC
  TCCATCCCCAACCAGCTGTTCATCAACCTTACGGACTTGCTGTACCTGGACCTCAGTGAAAACCGCCTAG
  AGAGCCTTCCCCCTCAAATGCGCCGCCTCGTGCACCTTCAGACACTGGTGCTGAATGGAAACCCGCTGCT
  ACATGCACAGCTCCGGCAGCTCCCAGCCATGATGGCCCTACAGACCCTGCATCTGAGGAACACTCAACGC
  ACCCAGAGCAACCTCCCCACCAGCCTGGAGGGCCTGAGCAACCTTTCAGATGTGGACCTGTCGTGCAATG
  ACTTGACAAGGGTGCCGGAGTGCCTGTACACCCTCCCCAGCCTGCGCCGCCTCAACCTCAGTAGCAACCA
  GATTGCAGAGCTGTCCCTGTGCATCGACCAGTGGGTGCACCTGGAGACCTTGAATCTGTCCCGGAACCAG
  CTCACCTCCCTGCCTTCAGCCATCTGCAAGCTCACCAAGCTGAAGAAGCTGTACCTCAACTCCAACAAGC
  TGGACTTTGATGGGCTGCCTTCTGGCATCGGCAAGCTCACCAGCCTGGAGGAATTCATGGCTGCCAACAA
  CAACCTAGAACTGATTCCTGAAAGTCTCTGCAGGTGCCCAAAACTGAAGAAACTCGTCCTGAATAAGAAC
  CGCCTGGTGACCCTTCCTGAGGCCATCCACTTCCTGACAGAGATCCAGGTCCTGGATGTTCGAGAAAACC
  CCAGCTTGGTCATGCCGCCCAAGCCTGCTGACCGCACCGCCGAGTGGTACAACATTGACTTCTCGCTACA
  GAACCAGCTGAGGCTGGCGGGCGCCTCCCCGGCCACAGTGGCTGCTGCTGCAGCTGGGAGCGGGTCCAAG
  GACCCCTTGGCTCGCAAGATGCGGCTACGGAGGCGCAAGGACTCAGCACAGGATGTCCAGGCCAAGCAAG
  TGCTAAAGGGCATGTCAGATGTTGCCCAGGAGAAAAACAAAAACCAAGAGGAAAGCATAGATGCCCGGGC
  ACCAGGGGGAAAGGTACGACGCTGGGACCAGGGCCTGGAGAAGCCACGCCTTGACTACTCGGAGTTCTTT
  ACGGAGGATGTGGGCCAGCTGCCTGGTTTGACCATCTGGCAAATTGAGAACTTTGTGCCTGTTCTGGTGG
  AAGAAGCCTTCCATGGCAAGTTCTACGAGGCTGACTGCTACATTGTGCTCAAGACCTTCCTGGATGACAG
  CGGCTCCCTGAACTGGGAGATCTACTACTGGATTGGTGGGGAGGCCACACTTGACAAGAAAGCCTGTTCC
  GCCATTCATGCAGTCAATCTGCGCAACTACCTGGGTGCTGAGTGCCGTACTGTTCGAGAGGAGATGGGTG
  ACGAGAGTGAGGAGTTCCTGCAGGTGTTTGACAATGACATCTCCTACATTGAAGGCGGAACAGCCAGTGG
  CTTCTATACTGTGGAAGATACACACTATGTTACCAGGATGTACCGTGTATATGGGAAAAAGAACATCAAA
  TTGGAGCCTGTGCCCCTCAAAGGGTCCTCACTAGATCCAAGGTTTGTGTTTCTCCTGGACCAAGGGCTGG
  ACATTTATGTGTGGCGGGGGGCCCAGGCCACACTGAGTAACACGACTAAGGCCAGGCTCTTTGCAGAGAA
  AATAAACAAGAATGAGCGGAAAGGGAAGGCGGAAATCACACTCCTGGTGCAGGGCCAAGAGCCCCCAGGG
  TTCTGGGATGTGCTGGGTGGAGAGCCTTCTGAGATCAAGAATCATGTGCCTGATGACTTTTGGCCACCCC
  AGCCCAAGCTATACAAGGTGGGCCTGGGTCTGGGCTACTTGGAGCTTCCGCAGATCAACTACAAGCTCTC
  AGTGGAACACAAAAAACGGCCCAAGGTGGAGCTGATGCCAGGAATGAGGCTGCTGCAGAGCCTACTGGAC
  ACACGATGCGTGTACATCCTGGACTGCTGGTCTGATGTATTCATCTGGCTGGGCCGCAAGTCCCCACGCC
  TAGTGCGTGCAGCAGCACTCAAACTTGGCCAGGAGCTATGTGGGATGCTACACCGGCCACGACATACAGT
  GGTCAGCCGCAGCCTGGAGGGCACCGAGGCCCAGGTGTTCAAGGCCAAGTTCAAGAACTGGGACGATGTG
  TTGACAGTGGACTACACACGGAATGCAGAGGCTGTGCTTCAGGGCCAGGGCCTCTCTGGGAAGGTGAAGC
  GTGACACTGAGAAGACAGACCAGATGAAGGCTGACCTCACTGCACTCTTCCTACCTCGGCAGCCCCCCAT
  GCCCCTGGCTGAGGCAGAGCAGCTGATGGAGGAGTGGAATGAGGACCTGGATGGCATGGAGGGCTTCGTG
  CTGGAGGGCCGCAAGTTCACTCGACTGCCAGAGGAGGAGTTTGGCCACTTCTACACACAGGACTGCTATG
  TCTTCCTCTGCAGGTACTGGGTACCTGTGGAGTACGAGGAGGAAGAGAAGACAGAAGACAAGGAGGGGAA
  GGCCTCGGCAGAGGCCAGAGAAGGGGAGGAAGCAGCAGCGGAGGCAGAGGAGAAGCAGCCAGAGGAGGAC
  TTTCAGTGCATCGTTTACTTCTGGCAGGGCCGGGAGGCCTCCAACATGGGCTGGCTGACCTTCACATTCA
  GTCTTCAGAAGAAGTTTGAGAGCCTCTTCCCTGGCAAGCTGGAGGTAGTACGTATGACACAGCAGCAGGA
  GAACCCCAAGTTCCTATCCCATTTCAAAAGAAAGTTCATCATTCACCGAGGCAAGAGGAAGGTGACCCAG
  GGTACTCTTCAGCCGACCCTCTATCAGATCCGCACCAACGGCAGTGCCCTCTGCACCCGGTGCATCCAGA
  TTAACACTGACTCCAGCCTTCTCAACTCTGAGTTCTGCTTCATCCTCAAGGTCCCCTTTGAAAGCGAGGA
  CAACCAAGGCATTGTGTATGCCTGGGTAGGTCGGGCATCAGACCCCGACGAAGCCAAGCTGGCAGAGGAC
  ATCCTGAACACTATGTTTGATGCATCCTACAGCAAACAGGTCATCAATGAAGGCGAGGAGCCAGAGAACT
  TCTTCTGGGTAGGCATTGGTGCACAGAAACCCTATGATGACGATGCGGAGTACATGAAGCACACGAGGCT
  CTTCAGGTGCTCCAATGAGAAGGGTTACTTCGCAGTGACTGAGAAATGCTCTGACTTTTGCCAAGATGAC
  CTGGCAGACGATGACATCATGCTGCTAGACAATGGCCAAGAAGTCTACATGTGGGTTGGGACCCAGACAA
  GCCAAGTGGAGATCAAACTGAGTCTGAAGGCTTGCCAGGTATACATCCAGCACACACGCTCTAAAGAACA
  TGAGCGGCCACGTCGCCTGCGCCTGGTCCGCAAAGGTAATGAGCAGCGCGCCTTCACCCGCTGCTTCCAC
  GCCTGGAGCACGTTCCGCCAAGCCCCAGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231745 representing NM_001302207
  Red=Cloning site Green=Tags(s)
  
  MEATGVLPFVRGVDLSGNDFKGGYFPENVKAMTSLRWLKLNRTGLCYLPEELAALQKLEHLSVSHNHLTT
  LHGELSSLPSLRAIVARANSLKNSGVPDDIFKLDDLSVLDLSHNQLTECPRELENAKNMLVLNLSHNGID
  SIPNQLFINLTDLLYLDLSENRLESLPPQMRRLVHLQTLVLNGNPLLHAQLRQLPAMMALQTLHLRNTQR
  TQSNLPTSLEGLSNLSDVDLSCNDLTRVPECLYTLPSLRRLNLSSNQIAELSLCIDQWVHLETLNLSRNQ
  LTSLPSAICKLTKLKKLYLNSNKLDFDGLPSGIGKLTSLEEFMAANNNLELIPESLCRCPKLKKLVLNKN
  RLVTLPEAIHFLTEIQVLDVRENPSLVMPPKPADRTAEWYNIDFSLQNQLRLAGASPATVAAAAAGSGSK
  DPLARKMRLRRRKDSAQDVQAKQVLKGMSDVAQEKNKNQEESIDARAPGGKVRRWDQGLEKPRLDYSEFF
  TEDVGQLPGLTIWQIENFVPVLVEEAFHGKFYEADCYIVLKTFLDDSGSLNWEIYYWIGGEATLDKKACS
  AIHAVNLRNYLGAECRTVREEMGDESEEFLQVFDNDISYIEGGTASGFYTVEDTHYVTRMYRVYGKKNIK
  LEPVPLKGSSLDPRFVFLLDQGLDIYVWRGAQATLSNTTKARLFAEKINKNERKGKAEITLLVQGQEPPG
  FWDVLGGEPSEIKNHVPDDFWPPQPKLYKVGLGLGYLELPQINYKLSVEHKKRPKVELMPGMRLLQSLLD
  TRCVYILDCWSDVFIWLGRKSPRLVRAAALKLGQELCGMLHRPRHTVVSRSLEGTEAQVFKAKFKNWDDV
  LTVDYTRNAEAVLQGQGLSGKVKRDTEKTDQMKADLTALFLPRQPPMPLAEAEQLMEEWNEDLDGMEGFV
  LEGRKFTRLPEEEFGHFYTQDCYVFLCRYWVPVEYEEEEKTEDKEGKASAEAREGEEAAAEAEEKQPEED
  FQCIVYFWQGREASNMGWLTFTFSLQKKFESLFPGKLEVVRMTQQQENPKFLSHFKRKFIIHRGKRKVTQ
  GTLQPTLYQIRTNGSALCTRCIQINTDSSLLNSEFCFILKVPFESEDNQGIVYAWVGRASDPDEAKLAED
  ILNTMFDASYSKQVINEGEEPENFFWVGIGAQKPYDDDAEYMKHTRLFRCSNEKGYFAVTEKCSDFCQDD
  LADDDIMLLDNGQEVYMWVGTQTSQVEIKLSLKACQVYIQHTRSKEHERPRRLRLVRKGNEQRAFTRCFH
  AWSTFRQAPA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001302207
• ORF Size: 3810 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001302207.1, NP_001289136.1
• RefSeq Size: 4068
• RefSeq ORF: 3813
• Locus ID: 14248
• MW: 145.2 kDa
• Gene Summary: This gene encodes a protein with gelsolin-like repeats and an N-terminal leucine-rich repeat domain. The protein is similar to a Drosophila protein involved in early embryogenesis and the structural organization of indirect flight muscle. This protein may act as an actin-remodelling protein as well as a transcriptional coactivator. Homozygous knockout mice show embryonic lethality. This protein may act to regulate wound repair. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014]