Edc4 (NM_001301100) Mouse Tagged ORF Clone

CAT#: MR231792

Edc4 (myc-DDK-tagged) - Mouse enhancer of mRNA decapping 4 (Edc4), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Edc4
• Synonyms: BC022641
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231792 representing NM_001301100
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTCCTGCGCGAGCATCGACATTGAGGACGCCACGCAGCATCTGCGGGACATACTCAAACTGGACC
  GGCCCGCCGGAGGTTCCAATGCCGAAAGCCAAAGGCCATCCAGTGCCTACAATGGAGACCTCAATGGGCT
  CCTGGTCCCCGACCCGCTCAGTTCAGGTGATGGTAACTCAACGAACAAACCTGGTATACGGACCATGCCA
  CCCATTAACCTGCAGGAAAAGCAGGTCATTTGCCTCTCTGGAGATGACAGCTCCACCTGCATTGGGATTT
  TAGCCAAGGAAGTGGAAATTGTGGCCAGCAGTGATTCTAGCATTTCCAGCAAGGCTCGGGGGAGCAACAA
  GGTGAAAATCCAGCCTGTCGCCAAGTATGACTGGGAGCAGAAATACTACTATGGCAACCTGATTGCAGTG
  TCTAACTCCTTCTTGGCATACGCCATTAGGGCTGCCAACAATGGCTCAGCAATGGTCCGTGTGATCAGTG
  TCAGCACTTCGGAGCGGACCCTGCTCAAAGGTTTCACAGGCAGTGTGGCTGATCTTGCTTTTGCACACCT
  CAACTCTCCACAGCTCGCCTGCCTGGATGAGGCTGGCAATCTTTTTGTGTGGCGCTTGGCTCTTGTTAAG
  GGTAAAATTCAAGAAGAGATTTTAGTCCATATCCGGCAGCCAGAGGGTACAGCGCTGAACCACTTCCGAA
  GGATCATCTGGTGCCCTTTCATCCCCGAGGAGAGTGAGGACTGTTGTGAGGAGAGCAGCCCGACAGTGGC
  CCTGCTGCATGAGGATCGGGCTGAGGTGTGGGACTTGGATATGCTTCGCTCCAGCCACAACACCTGGCCT
  GTGGATGTCAGCCAAATCAAGCAAGGCTTTATTGTGGTGAAAGGCCATAGCACGTGCCTAAGTGAAGGAG
  CCCTCTCCCCTGATGGGACTGTCCTGGCTACCGCAAGCCATGATGGCTTTGTCAAGTTCTGGCAGATCTA
  TATTGAAGGTCAGGATGAGCCAAGGTGTCTGCATGAATGGAAGCCTCATGATGGGAGGCCTCTTTCTTGC
  CTCCTGTTCTGTGATAACCATAAGAAACAGGATCCTGAGGTCCCGTTCTGGAGGTTCCTCATAACCGGCG
  CCGACCAGAATCGAGAGCTGAAGATGTGGTGCACAGTGTCCTGGACCTGCCTGCAAACCATTCGCTTCTC
  CCCAGATATCTTCAGCTCAGTGAGTGTGCCTCCCAGCCTCAAAGTTTGTTTGGACCTCTCAGCAGAGTAT
  TTGATTCTTAGTGATGTGCAACGAAAGGTCCTCTATGTGATGGAGCTGCTGCAGAACCAGGACGAGGGCC
  GCGCGTGCTTTAGTTCTATCTCTGAGTTCCTGCTCACCCACCCTGTGCTGAGCTTTGGCATCCAGGTTGT
  CAGTCGCTGCCGGCTACGGCACACTGAGGTCCTGCCTGCTGAGGAGGAGAATGACAGTCTGGGGACCGAG
  AGTTCCCATGGAGCTGGAGCCTTGGAATCCGCAGCTGGTGTGCTTATCAAGCTCTTTTGTGTGCACACTA
  AGGCACTACAAGATGTGCAGATCCGCTTCCAGCCACAGCTGAACCCTGATGTGGTGGCCCCACTCTCTAC
  CCACACTGCCCACGAGGACTTCACATTTGGAGAGTCTCGACCTGAACTAGGCTCCGAGGGGCTAGCTTCA
  GCTGCTCATGGTTCCCAGCCTGACCTGCGGCGCATTGTGGAACTGCCTGCTCCTGCAGACTTCCTCAGTC
  TGAGCAGTGAGACCAAGCCCAAGTTGATGACACCTGATGCTTTCATGACACCTACTGCCTCCCTGCAGCA
  GATCTCTGCATCCCCTAGTAGTAGCAGCAGCAGTAGCAGCAGCAGCAGTAGCAGCAGCAGCAGCAGCAGC
  AGCTCTCTAACAGCTGTGTCTGCTGTGAGCAGCTCCTCAGCCATGGACCCTTCCTTGCCCAGACCACCTG
  AGGAGCTGACCTTGAGCCCCAAGCTGCAGCTAGATGGCAGCCTGACGCTAAACAGCAGCAGCAGCAGCCT
  GCAGGCAAGCCCTCGAAGCCTCCTTCCGGGCCTGCTCCCAGGCCCAGCTGACAAGTTGATTTCCAAGGGA
  CCTGGTCAGGTATCCACTGCTGCCTCTGCACTGTCCTTGGATTTGCAGGAAGTGGAGCCATTAGGGCTAC
  CCCAGGCCTCCCCAAGTCGAACCCGTTCTCCTGATGTGATCTCCTCAGCATCCACTGCCCTGTCCCAGGA
  CATTCCTGAAATCGCATCTGAGGCCCTGTCCCGTGGCTTTGGCTCCTCTGTTCCTGAGGGCCTCATTGAG
  CCAGACAGCATGGCCTCAGCTGCCTCAGCACTACACCTACTCTCTCCTCGGCCCAGGCAAGGCCCTGAGC
  TTGGCTCTCAGCTGGGTCTGGATGGAGGCCCTGGAGATGGGGATAGGCATAGTACCCCTTCCCTGCTGGA
  AGCAGCCCTGACCCAGGAAGTTGCAACCCCTGACAGTCAAGTCTGGCCTACGGCACCTGACATTACTCGT
  GAGACCTGTAGCACACTGACAGAAAGCCCCAGGAATGGCCTCCAGGAAAAGCACAAAAGTCTGGCTTTCC
  ACAGGCCACCTTATCACTTGCTGCAGCAACGTGACAGTCAGGACACAAGTGCTGAGCAAAGTGACCATGA
  TGATGAGGTTGCCAGCCTTGCCTCTGCCTCAGGAGGTTTTGGCAGCAAAATTCCTACTCCACGGCTGCCT
  TCCAAGGATTGGAAGACGAAGGGATCCCCTCGGACGTCACCTAAGCTCAAGAGAAAAAGCAAGAAGGATG
  ATGGGGATTCAGCTGTGGGATCCCGGCTCACAGAGCACCAGGTGGCAGAGCCCCCTGAAGACTGGCCAGC
  ACTAATTTGGCAGCAGCAAAGAGAGCTGGCAGAGCTATGGCACAACCAAGAAGAACTGCTACAGCGTCTC
  TGTGCCCAGCTTGAAGGTCTTCAGAGTACTGTCACAGACCATGTAGAGCGTGCCCTGGAGACGCGGCATG
  AGCAGGAGCAACGGCGGCTGGAACGGGCACTGGCTGAGGGGCAGCAACGGGGTGGGCAGCTGCAGGAGCA
  GCTGACACAGCAGCTATCCCAGGCATTGTCTTCAGCTGTGGCTGGGCGGCTAGAGCGCAGCGTAAGGGAT
  GAAATCAAGAAAACGGTTCCTCCATGTGTCTCCAGAAGTCTGGAGCCTGTTGCAGGTCAACTAAGCAACT
  CAGTGGCTACCAAGCTTACAGCTGTGGAAGGCAGCATGAAAGAGAATATCTCTAAGCTACTCAAGTCCAA
  GAACTTAACAGATGCCATTGCCCGAGCAGCTGCAGACACGTTACAGGGACCAATGCAGGCTGCCTACCGG
  GAAGCCTTCCAGAGTGTGGTACTGCCAGCTTTTGAGAAGAGTTGCCAGGCTATGTTCCAGCAAATCAATG
  ACAGCTTTAGGCTGGGCACACAGGAATATTTGCAGCAGCTGGAGAGTCACATGAAGAGCCGGAAGGCCCG
  TGAACAAGAAGCTAGGGAGCCTGTGCTGGCCCAGCTTCGGGGCCTGGTCAGCACACTGCAGAGTGCCACC
  GAGCAGATGGCGGCCACTGTATCTAGCAGTGTTCGGGCCGAGGTGCAACACCAGCTGCATGTGGCTGTGG
  GCAGCTTGCAGGAGTCAATCTTAGCACAAGTACAGCGCATTGTCAAGGGTGAAGTGAGTGTGGCACTTAA
  GGAGCAGCAGGCCACCGTCACTTCCAGCATCATGCAGGCTATGCGTTCAGCTGCTGGCACACCTGTCCCC
  TCTGCCCACCTTGACTGCCAGGCCCAACAAGCCCATATCTTGCAGTTACTGCAGCAGGGCCACCTCAATC
  AGGCCTTCCAGCAGGCACTGACTGCTGCTGATCTCAATCTGGTGCTGTATGTGTGTGAAACTGTCGACCC
  AGCCCAGGTTTTTGGGCAGCCGCCCTGTCCACTATCCCAGCCTGTTCTCCTTTCCCTAATCCAGCAGCTA
  GCATCTGACCTTGGAACTAGAAGTGACCTCAAGCTCAGCTACTTGGAAGAAGCTGTGATGCACCTGGACC
  ACAGCGATCCCATCACTCGAGACCACATGGGCTCTGTCATGGCTCAGGTGCGCCAGAAGCTCTTCCAGTT
  CCTGCAGGCTGATCCACACAACTCACTTAGCAAAGCTGCCCGCCGCCTCAGCCTAATGTTACACGGCCTT
  GTGACCCCTAGCCTCCCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231792 representing NM_001301100
  Red=Cloning site Green=Tags(s)
  
  MASCASIDIEDATQHLRDILKLDRPAGGSNAESQRPSSAYNGDLNGLLVPDPLSSGDGNSTNKPGIRTMP
  PINLQEKQVICLSGDDSSTCIGILAKEVEIVASSDSSISSKARGSNKVKIQPVAKYDWEQKYYYGNLIAV
  SNSFLAYAIRAANNGSAMVRVISVSTSERTLLKGFTGSVADLAFAHLNSPQLACLDEAGNLFVWRLALVK
  GKIQEEILVHIRQPEGTALNHFRRIIWCPFIPEESEDCCEESSPTVALLHEDRAEVWDLDMLRSSHNTWP
  VDVSQIKQGFIVVKGHSTCLSEGALSPDGTVLATASHDGFVKFWQIYIEGQDEPRCLHEWKPHDGRPLSC
  LLFCDNHKKQDPEVPFWRFLITGADQNRELKMWCTVSWTCLQTIRFSPDIFSSVSVPPSLKVCLDLSAEY
  LILSDVQRKVLYVMELLQNQDEGRACFSSISEFLLTHPVLSFGIQVVSRCRLRHTEVLPAEEENDSLGTE
  SSHGAGALESAAGVLIKLFCVHTKALQDVQIRFQPQLNPDVVAPLSTHTAHEDFTFGESRPELGSEGLAS
  AAHGSQPDLRRIVELPAPADFLSLSSETKPKLMTPDAFMTPTASLQQISASPSSSSSSSSSSSSSSSSSS
  SSLTAVSAVSSSSAMDPSLPRPPEELTLSPKLQLDGSLTLNSSSSSLQASPRSLLPGLLPGPADKLISKG
  PGQVSTAASALSLDLQEVEPLGLPQASPSRTRSPDVISSASTALSQDIPEIASEALSRGFGSSVPEGLIE
  PDSMASAASALHLLSPRPRQGPELGSQLGLDGGPGDGDRHSTPSLLEAALTQEVATPDSQVWPTAPDITR
  ETCSTLTESPRNGLQEKHKSLAFHRPPYHLLQQRDSQDTSAEQSDHDDEVASLASASGGFGSKIPTPRLP
  SKDWKTKGSPRTSPKLKRKSKKDDGDSAVGSRLTEHQVAEPPEDWPALIWQQQRELAELWHNQEELLQRL
  CAQLEGLQSTVTDHVERALETRHEQEQRRLERALAEGQQRGGQLQEQLTQQLSQALSSAVAGRLERSVRD
  EIKKTVPPCVSRSLEPVAGQLSNSVATKLTAVEGSMKENISKLLKSKNLTDAIARAAADTLQGPMQAAYR
  EAFQSVVLPAFEKSCQAMFQQINDSFRLGTQEYLQQLESHMKSRKAREQEAREPVLAQLRGLVSTLQSAT
  EQMAATVSSSVRAEVQHQLHVAVGSLQESILAQVQRIVKGEVSVALKEQQATVTSSIMQAMRSAAGTPVP
  SAHLDCQAQQAHILQLLQQGHLNQAFQQALTAADLNLVLYVCETVDPAQVFGQPPCPLSQPVLLSLIQQL
  ASDLGTRSDLKLSYLEEAVMHLDHSDPITRDHMGSVMAQVRQKLFQFLQADPHNSLSKAARRLSLMLHGL
  VTPSLP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001301100
• ORF Size: 4218 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001301100.1, NP_001288029.1
• RefSeq Size: 4803
• RefSeq ORF: 4221
• Locus ID: 234699
• MW: 152.9 kDa
• Gene Summary: The protein encoded by this gene is thought to promote mRNA decay, and is known to interact with several mRNA decapping proteins. In humans, decreased expression of this gene prevents the accumulation of mRNA decapping proteins to mRNA processing bodies (P-body). Alternative splicing results in multiple protein isoforms. [provided by RefSeq, Jul 2014]