Ank1 (NM_001277280) Mouse Tagged ORF Clone

CAT#: MR231871

Ank1 (myc-DDK-tagged) - Mouse ankyrin 1, erythroid (Ank1), transcript variant 3

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ank1
• Synonyms: Ank-1; nb; pale
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231871 representing NM_001277280
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCAGGCGGCCAAACAGCTGAAGAAAATCAAAGACATCGAGGCGCAGGCCCTCCAGGAGCAGAAGG
  AGAAGGAGGAATCCAACAGGAAGCGGCGGAACCGCTCCCGTGACCGCAAGAAGAAGGCCGATGCTGCTAC
  CAGCTTTCTGCGGGCGGCACGCTCGGGGAACCTGGACAAGGCTCTGGATCACCTGCGCAATGGAGTGGAC
  ATTAACACCTGTAACCAGAACGGGTTGAACGGCCTGCATCTGGCCTCCAAAGAAGGCCATGTGAAGATGG
  TGGTTGAACTTCTGCACAAAGAGATCATTCTAGAAACGACAACCAAGAAGGGGAACACTGCTCTGCACAT
  CGCTGCCCTTGCTGGTCAGGATGAGGTGGTCCGGGAGCTGGTCAACTATGGAGCCAATGTCAATGCCCAG
  TCTCAGAAAGGCTTTACTCCCCTGTACATGGCTGCTCAGGAGAACCACTTGGAAGTGGTGAAATTTCTAC
  TGGAGAATGGAGCCAATCAGAATGTAGCCACAGAAGATGGCTTCACCCCACTGGCCGTGGCTCTACAGCA
  GGGTCACGAGAATGTGGTGGCTCACCTCATCAACTATGGGACGAAAGGGAAAGTGCGCCTCCCTGCCCTG
  CACATCGCGGCCCGCAACGATGACACACGGACAGCCGCAGTCCTTCTGCAGAATGACCCCAACCCAGATG
  TGCTTTCCAAGACGGGATTCACACCCCTCCACATCGCAGCTCACTATGAGAACCTCAACGTGGCCCAGTT
  GCTCCTCAACAGGGGAGCCAGCGTCAACTTCACACCTCAGAATGGCATCACCCCACTACACATCGCCTCC
  CGCAGGGGGAACGTGATCATGGTGAGACTCCTGCTGGACCGAGGGGCTCAGATAGAAACGAGGACCAAGG
  ATGAATTGACACCGCTCCACTGTGCAGCTCGGAATGGACACGTGAGAATCTCAGAGATCCTGCTGGACCA
  CGGGGCACCCATCCAAGCCAAAACCAAGAATGGCTTGTCCCCAATCCACATGGCCGCTCAGGGAGACCAC
  CTCGACTGTGTCCGACTTCTATTGCAATACAATGCAGAGATAGACGACATCACCTTGGATCACCTGACTC
  CTCTCCATGTGGCAGCCCACTGTGGCCACCACCGGGTGGCTAAGGTTCTTTTGGATAAAGGGGCCAAGCC
  CAACTCCAGAGCCCTGAATGGTTTTACCCCGTTACACATCGCCTGCAAGAAGAACCACATCCGTGTAATG
  GAGTTGCTGCTGAAGACAGGAGCCTCCATCGACGCGGTCACTGAGTCTGGCCTGACACCTCTCCACGTAG
  CCTCCTTCATGGGACACCTTCCTATTGTGAAGAACTTACTGCAGCGGGGAGCGTCACCCAATGTCTCCAA
  TGTGAAAGTAGAAACCCCCTTGCACATGGCAGCCCGAGCAGGGCATACAGAAGTGGCCAAATATTTGCTC
  CAGAACAAAGCCAAAGCCAACGCCAAGGCCAAGGATGACCAGACACCGCTTCACTGTGCTGCTCGAATCG
  GCCACACAGGCATGGTGAAGCTCCTGCTGGAGAATGGTGCCAGCCCCAATCTGGCTACCACTGCTGGCCA
  CACACCCCTACACACCGCAGCCCGTGAGGGACACGTGGACACAGCCCTGGCCCTGCTGGAGAAGGAGGCA
  TCCCAAGCCTGCATGACCAAGAAAGGATTTACCCCTCTGCACGTGGCTGCTAAGTACGGGAAGGTACGGT
  TGGCGGAGCTGCTGCTGGAACACGATGCACACCCCAATGCAGCTGGGAAGAACGGCTTGACTCCTCTGCA
  TGTGGCCGTCCATCACAACAACCTGGACATTGTCAAACTTCTTCTTCCCCGAGGTGGCTCCCCCCACAGC
  CCTGCCTGGAATGGCTACACTCCTTTGCACATCGCTGCCAAGCAGAACCAGATAGAGGTGGCCCGCAGTC
  TACTGCAGTACGGAGGGTCAGCGAATGCAGAGTCGGTACAAGGCGTGACCCCACTTCACCTGGCTGCCCA
  AGAGGGCCACACAGAAATGGTTGCTCTTCTCCTGTCCAAGCAAGCCAACGGCAACCTGGGGAACAAGAGT
  GGACTCACTCCCCTCCACCTGGTGTCACAAGAAGGCCATGTTCCAGTGGCAGACGTGCTGATCAAACATG
  GCGTCACCGTGGATGCTACCACCCGGATGGGTTACACCCCGCTCCATGTGGCCAGTCATTATGGAAACAT
  CAAGCTGGTGAAGTTCTTGCTACAGCACCAGGCAGATGTCAATGCCAAGACCAAGCTAGGATACAGCCCC
  TTGCACCAGGCAGCTCAACAAGGACACACAGACATTGTGACATTGCTCCTGAAGAATGGTGCTTCTCCAA
  ATGAGGTCAGCTCGAACGGAACCACACCTCTGGCAATAGCCAAACGTTTGGGCTACATCTCTGTAACAGA
  CGTGCTCAAGGTGGTCACAGATGAAACCAGTGTGGTGTTAGTCAGTGACAAACACCGGATGAGCTACCCT
  GAGACGGTGGACGAGATCCTGGACGTGTCTGAAGATGAAGGTGACGAACTTGTCGGCTCGAAGGCTGAAA
  GGCGGGACTCCCGGGATGTGGGTGAGGAGAAAGAGCTGTTGGATTTTGTGCCGAAGCTAGACCAAGTGGT
  GGAGTCTCCAGCCATCCCAAGGATCCCTTGTGTCACACCTGAGACCGTTGTGATCCGATCTGAAGATCAG
  GAACAAGCATCTAAAGAATATGATGAGGATTCCCTCATTCCCAGCAGCCCGGCCACAGAGACCTCAGACA
  ACATTAGCCCTGTGGCTAGCCCGGTCCACACAGGGTTCCTGGTGAGCTTCATGGTGGATGCCCGAGGGGG
  ATCCATGAGAGGAAGCCGTCATAATGGCCTCAGGGTGGTGATCCCTCCCCGGACATGCGCAGCACCCACT
  CGCATCACCTGCCGCCTCGTTAAGCCGCAGAAGCTGAACACGCCACCCCCACTGGCTGAGGAAGAGGGCC
  TAGCCAGCAGGATCATTGCCCTGGGACCCACGGGGGCCCAGTTCCTTAGCCCTGTGATCGTGGAAATCCC
  CCATTTTGCCTCCCATGGCCGTGGGGACCGTGAGCTGGTGGTTCTGAGGAGTGAAAACGGCTCTGTGTGG
  AAGGAGCACAAGAGTCGTTATGGGGAAAGCTACCTGGACCAGATCCTTAATGGCATGGATGAAGAGCTGG
  GGAGCCTGGAGGAGCTGGAGAAGAAACGTGTGTGCCGCATCATCACCACCGACTTCCCTCTGTACTTTGT
  CATCATGTCCCGGCTCTGCCAGGACTACGACACCATCGGTCCTGAAGGGGGTTCCCTGAGAAGCAAGCTG
  GTACCGCTGGTACAGGCAACGTTCCCTGAAAACGCTGTGACCAAGAAAGTGAAGCTGGCTCTGCAGGCCC
  AGCCTGTCCCGGATGAGCTGGTGACCAAGCTCCTGGGTAACCAGGCCACATTCAGCCCCATTGTGACCGT
  GGAGCCGAGGCGTCGGAAGTTCCACCGTCCCATCGGGCTGCGAATCCCGCTCCCTCCCTCATGGACGGAC
  AACCCCAGGGACAGTGGGGAGGGAGACACCACCAGCCTGCGCCTACTGTGTAGCGTCATCGGTGGAACTG
  ACCAAGCCCAGTGGGAAGATATAACGGGAACAACCAAACTTATATACGCCAATGAGTGTGCCAACTTTAC
  CACCAATGTCTCAGCCAGGTTCTGGCTGTCAGACTGTCCCCGGACTGCGGAGGCTGTGCACTTTGCCACT
  CTCCTGTACAAAGAGCTCACAGCAGTCCCCTACATGGCTAAGTTTGTCATTTTTGCCAAGATGAACGACG
  CTCGGGAAGGACGGCTCCGTTGCTACTGCATGACGGATGACAAAGTGGACAAGACCCTGGAGCAGCATGA
  AAACTTCGTGGAGGTGGCCCGGAGCAGGGACATAGAGGTTCTGGAAGGGATGCCTCTGTTTGCAGAACTC
  TCTGGGAACTTGGTTCCTGTCAAGAAAGCGGCCCAACAGCGGAGCTTCCATTTCCAGTCGTTTCGAGAGA
  ATCGTCTGGCCATCCCCGTGAAGGTGAGGGACAGCAGCCGCGAGCCAGGAGGGTTCTTGTCGTTCCTACG
  AAAGACAATGAAGTACGAGGACACACAGCACATCCTCTGTCACCTGAATATCACCATGCCCCCCTGCACC
  AAGGGCAGTGGAGCAGAAGACAGGAGAAGGACCCTGACACCCCTGACCCTTCGATACAGCATTCTCAGCG
  AGTCGCGGCTGGGTTTTACCAGTGACACAGACCGTGTCGAAATGAGGATGGCTGTCATCAGAGAACACCT
  CGGCCTAAGCTGGGCAGAGCTGGCCCGGGAGCTGCAGTTCAGCGTGGAAGACATCAACCGGATCCGTGTA
  GAAAACCCTAACTCCTTGTTGGACCAGAGTACAGCCTTGCTGACCCTCTGGGTGGACCGTGAAGGCGAAA
  ATGCAAAGATGGAGAATTTGTACACAGCCCTGCGGAACATCGACAGGAGTGAGATTGTTAACATGTTGGA
  GGGCTCCGGCAGGCAGAGCAGAAACCTCAAACCAGAGCGGAGACATGGGGACCGGGAGTACTCATTGTCA
  CCCTCCCAGGTGAATGGCCAGCAGCGAGTTCACGCCCGAATCACAGACTCACCCTCAGTGAGGCAGGTGC
  TGGACAGAAGCCAGGCCAGAACACTGGACTGGGATAAACAGGGTTCCACAGCGGTACACCCGCAAGAAGC
  CACACAGAGCTCCTGGCAAGAGGAGGTCACGCAGGGCCCACACTCATTCCAGAGAAGGATCACCACCATC
  CAAGGGCCGGAGCCTGGTGCGCTTCAGGAATACGAGCAGGTGCTGGTGTCTACCAGGGAGCATGTGCAGA
  GGGGGCCACCTGAGACCGGCAGCCCCAAAGCTGGCAAGGAACCTAGCCTGTGGGCACCTGAGAGCGCCTT
  CTCTCAAGAGGTGCAGGGGGATGAGCTTCAGAATATTCCAGGAGAGCAGGTGACGGAGGAACAATTCACA
  GATGAACAGGGCAACATTGTTACCAAGAAGATCATTCGCAAAGTCGTCCGGCAGGTAGACTCGTCTGGTG
  CCATCGACACCCAGCAGCACGAGGAGGTGGAGCTAAGAGGGAGTGGACTCCAGCCGGACCTGATAGAGGG
  CAGGAAGGGGGCTCAGATAGTGAAGCGGGCCAGCCTGAAAAGGGGCAAGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231871 representing NM_001277280
  Red=Cloning site Green=Tags(s)
  
  MAQAAKQLKKIKDIEAQALQEQKEKEESNRKRRNRSRDRKKKADAATSFLRAARSGNLDKALDHLRNGVD
  INTCNQNGLNGLHLASKEGHVKMVVELLHKEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQ
  SQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPAL
  HIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIAS
  RRGNVIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDH
  LDCVRLLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHIRVM
  ELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLL
  QNKAKANAKAKDDQTPLHCAARIGHTGMVKLLLENGASPNLATTAGHTPLHTAAREGHVDTALALLEKEA
  SQACMTKKGFTPLHVAAKYGKVRLAELLLEHDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHS
  PAWNGYTPLHIAAKQNQIEVARSLLQYGGSANAESVQGVTPLHLAAQEGHTEMVALLLSKQANGNLGNKS
  GLTPLHLVSQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSP
  LHQAAQQGHTDIVTLLLKNGASPNEVSSNGTTPLAIAKRLGYISVTDVLKVVTDETSVVLVSDKHRMSYP
  ETVDEILDVSEDEGDELVGSKAERRDSRDVGEEKELLDFVPKLDQVVESPAIPRIPCVTPETVVIRSEDQ
  EQASKEYDEDSLIPSSPATETSDNISPVASPVHTGFLVSFMVDARGGSMRGSRHNGLRVVIPPRTCAAPT
  RITCRLVKPQKLNTPPPLAEEEGLASRIIALGPTGAQFLSPVIVEIPHFASHGRGDRELVVLRSENGSVW
  KEHKSRYGESYLDQILNGMDEELGSLEELEKKRVCRIITTDFPLYFVIMSRLCQDYDTIGPEGGSLRSKL
  VPLVQATFPENAVTKKVKLALQAQPVPDELVTKLLGNQATFSPIVTVEPRRRKFHRPIGLRIPLPPSWTD
  NPRDSGEGDTTSLRLLCSVIGGTDQAQWEDITGTTKLIYANECANFTTNVSARFWLSDCPRTAEAVHFAT
  LLYKELTAVPYMAKFVIFAKMNDAREGRLRCYCMTDDKVDKTLEQHENFVEVARSRDIEVLEGMPLFAEL
  SGNLVPVKKAAQQRSFHFQSFRENRLAIPVKVRDSSREPGGFLSFLRKTMKYEDTQHILCHLNITMPPCT
  KGSGAEDRRRTLTPLTLRYSILSESRLGFTSDTDRVEMRMAVIREHLGLSWAELARELQFSVEDINRIRV
  ENPNSLLDQSTALLTLWVDREGENAKMENLYTALRNIDRSEIVNMLEGSGRQSRNLKPERRHGDREYSLS
  PSQVNGQQRVHARITDSPSVRQVLDRSQARTLDWDKQGSTAVHPQEATQSSWQEEVTQGPHSFQRRITTI
  QGPEPGALQEYEQVLVSTREHVQRGPPETGSPKAGKEPSLWAPESAFSQEVQGDELQNIPGEQVTEEQFT
  DEQGNIVTKKIIRKVVRQVDSSGAIDTQQHEEVELRGSGLQPDLIEGRKGAQIVKRASLKRGKQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001277280
• ORF Size: 5232 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001277280.1, NM_001277280.2, NP_001264209.1
• RefSeq Size: 7794
• RefSeq ORF: 5235
• Locus ID: 11733
• MW: 192.5 kDa
• Gene Summary: Attaches integral membrane proteins to cytoskeletal elements; binds to the erythrocyte membrane protein band 4.2, to Na-K ATPase, to the lymphocyte membrane protein GP85, and to the cytoskeletal proteins fodrin, tubulin, vimentin and desmin. Erythrocyte ankyrins also link spectrin (beta chain) to the cytoplasmic domain of the erythrocytes anion exchange protein; they retain most or all of these binding functions. In skeletal muscle, isoform Mu7 together with obscurin may provide a molecular link between the sarcoplasmic reticulum and myofibrils. [UniProtKB/Swiss-Prot Function]