Wnk2 (NM_001290311) Mouse Tagged ORF Clone

CAT#: MR231932

Wnk2 (myc-DDK-tagged) - Mouse WNK lysine deficient protein kinase 2 (Wnk2), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Wnk2
• Synonyms: 1810073P09Rik; AW122246; ESTM15; mKIAA1760; X83337
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231932 representing NM_001290311
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGGCGATGGCGGCCGCCGAGACGCCCCCGGCGCGCTGATGGAAGCCGGGCGCGGCACGGGGTCGG
  CGGGCATGGCGGAGCCGCGGGCGAGGGCGGCGCGGCTCGGGCCCCAGCGCTTCCTGCGGCGCAGCGTGGT
  GGAGTCGGACCAGGAGGAGCCGCCGGGCCTGGAGGCGGCCGAGACGCCGAGCGCGCAGCCCCCACAGCCT
  CTGCAGCGCAGGGTGCTTCTACTCTGCAAGACGCGCCGGCTCATCGCGGAGCGCGCCCGGGGCCGCCCCG
  CCGCGCCCGCGCCCGCCGCGCCCGCTGCGCCTCCCGGCTCCCCGAGTGTCCCCTCGGACCCTGGCCCGGA
  GCGAGCGGGCACGCAGGAGCCCAGCCCAGATCCCACCACCGCCTCAGCCGCAGCCGCGCAGGTCCCCGAC
  GGCGGCCCGAGGCAGGAAGAGGCGCCGGCCCCAACACAGGAGGACGCTGGAACCACCGAGGCAAAGCCGG
  AGCCCGGGCGCGCACGCAAGGATGAACCTGAAGAGGAGGAAGACGATGAGGACGACCTCAAGGCCGTGGC
  CACCTCTTTGGACGGCCGCTTCCTCAAGTTCGACATCGAGCTGGGCCGGGGCTCCTTCAAGACGGTCTAC
  AAGGGGCTGGACACGGAGACCTGGGTGGAGGTAGCATGGTGTGAACTGCAGGACCGGAAGCTCACCAAGC
  TTGAACGGCAGAGATTCAAAGAGGAGGCGGAGATGCTGAAAGGTCTACAGCACCCCAACATTGTGCGCTT
  CTATGACTTCTGGGAGTCCAGTGCCAAGGGCAAGCGATGTATCGTGCTGGTGACAGAGCTGATGACCTCG
  GGTACACTGAAGACGTACCTGAAGCGGTTCAAGGTGATGAAGCCGAAGGTACTGAGGAGCTGGTGCCGGC
  AGATCCTGAAGGGCCTGCTGTTCCTGCATACAAGGACACCCCCCATCATCCACCGTGACCTCAAGTGTGA
  CAACATCTTCATCACTGGGCCCACCGGCTCTGTGAAGATTGGTGACTTGGGTCTGGCCACCCTTAAAAGA
  GCGTCTTTTGCCAAAAGTGTGATAGGTACCCCCGAGTTCATGGCACCTGAAATGTACGAGGAACATTACG
  ACGAGTCGGTTGATGTCTACGCCTTCGGGATGTGCATGCTGGAAATGGCTACCTCAGAGTACCCCTATTC
  GGAGTGCCAGAACGCCGCCCAGATCTATCGCAAGGTCACCTGTGGCATCAAACCGGCCAGCTTCGAGAAG
  GTGCATGATCCGGAGATTAAGGAGATCATCGGGGAGTGCATCTGTAAGAACAAAGAGGAGAGGTATGAGA
  TTAAGGACCTGCTGAGCCATGCCTTCTTCGCGGAAGACACGGGTGTCCGGGTGGAACTTGCCGAGGAGGA
  CCATGGCAGGAAGTCTACCATAGCCCTGCGGCTTTGGGTGGAAGACCCCAAGAAGCTGAAGGGCAAGCCC
  AAAGACAATGGAGCCATAGAGTTTACCTTTGACTTGGAGAAGGAGACTCCCGACGAGGTGGCCCAGGAAA
  TGATCGATTCTGGATTTTTCCATGAGAGTGATGTGAAGATTGTGGCCAAGTCCATCCGTGACCGGGTGGC
  ACTGATTCAGTGGCGGCGTGAGAGGATCTGGCCTGCACTGCAGTCTCAGGAGCCAAAGGACTCTGGCAGC
  CCTGACAAGGCCAGGGGTCTGCCAGCACCCCTGCAGGTCCAGGTGACCTACCACGCACAGAGTGGGCAGC
  CTGGGCAGCCAGAACCTGAGGAGCCTGAGGCCGACCAGCATCTCCTGCCCCCTACCTTGCCAGCTAGCGT
  TACCTCCTTAGCCTCGGACAGCACCTTTGACAGTGGCCAGGGCTCTACCGTGTACTCGGACTCCCAGAGC
  AGCCAGCAGAGCATGGTGCTCAGCTCGCTTGTGGACACGGCCCCAACCCCAGCCTCGTGCGTGTGCAGCC
  CCCCTGTGAGTGAGGGGCCTGGCCTGACCCACAGCCTGCCTACGCTAGGGGCCTTCCAGCAGCCGGCCAC
  TGTGCCTGGCCTGTCTGTGGGCCCTGTGCCACCCCCTGCCCGCCCTCCACTCCTCCAGCAGCATTTTCCA
  GAATCTTCCATGAGCTTCACCCCTGTGCTGCCACCACCCAGCACCCCTGTGCCCACGGGCCCAAGCCAGC
  CAGCACCCCCAGTCCAGCAGCCTCTTCCAATGGCCCAACCACCTACCCTGCCACAGGTCCTGGCCCCACA
  GCCCATGGGCACTGTCCAGCCAGTACCCTCTCATTTGCCTCCATACCTGGCTCCAACCTCCCAGGTCGTG
  GCCCCTGCTCAGCTGAAGCCTCTCCAGATGCCACAGCCACCTCTGCAACCTCTTGCTCAAGTCCCTCCGC
  AGATGCCTCAGATGCCTGTGGTCCCCCCCATCACCCCCTTAACAGGACTCGATGGCCTCCCTCAGACCCT
  CACTGACCTGCCGGCCGCAAACGTGGCCCCCGTGCCACCACCTCAGTATTTCTCACCTGCTGTGATCTTG
  CCAAGCCTCACAACACCCCTGCCCACATCACCGGCCCTGCCTATGCAGGCTGTCAAGTTGCCTCATCCCC
  CTGGAACACCCCTGGCTGTGCCCTGTCAGACCATCGTGCCAAATGCACCAGCTGCTATCCCCCTGTTGGC
  CGTGGCCCCGCAGGGTGTGGCTGCACTGTCTATCCATCCAGCGGTGGCCCAGATCCCAGCCCAGCCTGTG
  TACCCAGCAGCCTTTCCACAGATGGTACCTGGAGACATCCCACCCTCACCCCACCACACGGTGCAGAGCT
  TACGGGCCACCCCTCCACAGCTGGCCTCACCTGTGCCTCCTCAGCCTGTCCAGCCCAGTGTCATTCATCT
  ACCTGAGCAGGCTGCTCCAACTGCTGCCTCAGGGACCCAGGTCCTGCTGGGCCACCCTCCTTCATACACC
  GCGGACGTTGCTGCTCCAGTCTCTGCTGTGTCCCTGCCGCCTGCTGTCCTTTCCCCTCCTCTGCCAGATA
  CTCTGCTGCCTACTGTCCCTGACCTCCTGCCCAAGGTCCCCAGCTCTCTGGCCCCTACAGTGGTCGCAGC
  CTCTCAGAGTGCGCCCGCACAGACCTCTTCACTCCTCCTGCCAACAAACCCACCCCTGCCCACTGGGCCT
  GCAGTTGCTGGCCCCTGCCCTGCAGTCCAGCTGATGGTGGAAGTGGCTCAAGAGGAGCAGGTCTCACAAG
  ACAAACCCCCTGGCCCCCCACAGAGCTCCGAGAGCTTTGGAGGTTCTGATGTCACCTCTGGAAGAGATCT
  GAGTGACAGCTGTGAAGGCACCTTTGGAGGGGGCAGGCTGGAGGGAAGGACCGCCCGGAAACACCACCGT
  AGGTCCACTCGTGCACGCTCGCGGCAGGAGAGGGCCAGCCGGCCCCGGCTCACTATCCTAAATGTGTGTA
  ACACGGGGGACAAGATGGTGGAGTGTCAGCTGGAAACGCATAACCATAAGATGGTGACCTTCAAGTTTGA
  CCTCGATGGGGACGCTCCGGATGAGATTGCCACGTACATGGTAGAGCATGACTTTATCCTGCCCGCTGAG
  CGAGAGACCTTCATCGAGCAGATGAAGGATGTCATGGACAAGGCAGAAGACATGCTCAGTGAGGACACAG
  ATGCTGACCATGGCTCTGATACAGGGACCAGCCCTCCACACCTGGGTACCTGTGGTCTGGCCACGGGGGA
  GGAGAACAGACAGTCCCAAGCCAATGCCCCTGTCTACCAGCAGAATGTCTTACACACGGGAAAGAGGTGG
  TTTATCATCTGTCCAGTGGCTGAACACCCAGCAACAGACACTTCTGAATCATCACCCCCACTTCCTCTAA
  GCTCCTTGCAGCCAGAAGCCAGCCAAGACCCAGCGCCCTATCCAGACCAGCTGTCCTTGACGGACAAACC
  TAGTTTCCCAGCTGCTCAGCAGCTCCTGAGCCAGGCAGGCTCCAGCAACCCTCCTGGTGGAGCATCAGCC
  CCCTTGGCCCCGTCTTCCCCTCCTGTGACTACTGTAATCCCGGCAGCACCAGCCACCAGCACCGTGCCAG
  AGTCAGCAGCAGGGACTGCCATGCAGGCAGGAGGTCCAGGGACTCATCAAGGACCAGCCAGTGTCCATGA
  GACCCTCCAGCCACTGGCCGAGACACGCTCTGCCCAGTGTACTGCCCAGCCCCTTAGTACTGGCCAAGGA
  CCTTGCACCCCAGCTCTGGAAGCCTCTCGCTGCTCCACAGGTCTGGGGGAGCCCATCTCAACCAGGGAGG
  TCAGCACTCAAGGAGAGCCCCTGCCTGCTTCAGTGCCAGAACCTAGCCCTCCCACTGGGGCCACACAATC
  TGTACCGGGTCAGCCACCACCCCCACTGCCCATCACAGTAGGGGCTATCAGTCTGGCAGCTCCCCAGCTC
  CCCAGTCCCCCCCTGGGACCTACTGCTCCCCCACCACCCCCCTCAGCCTTGGAATCTGATGGGGAAGGGC
  CGCCCCCTAGGGTGGGCTTCGTGGACAACACCATCAAGAGTCTGGATGAGAAACTGCGGACTCTGCTCTA
  CCAGGAGCACGTGCCCACCTCCTCAGCCTCAGCTGGGACCCCCATGGAGGCAAGCGATAGAGACTTCACC
  CTGGAGCCCCTGCGAGGAGACCTTCCCTCAGCCTTGAGTGACAAGACCCCAAGCCTCACCCAGCAGACCC
  AGCCCTCATTGGAAAAGTCTGAAACGGCCCCTGCAGGATGGGCCCTGGCCCAGAGGGAACAGGGTGCATC
  TTCTCCAATGACAGCAGAGTCATCTTCCAGCAATACACTGGGCTGTGACAGTGACGCAGGGCAGGTGGCC
  TCGGACTCCTCCACAGCACCCAGTGTCCCCCAGGATGCCTCTGGTTCTTCCGTCCCTACACACATGGATC
  CCAAAGATCAGAACAGTAGTGTTCCCAGAGAAGCCTTGGCAGCACCCATGCAAAGTGGCCCAGGGTCCTT
  CACAGTGGGCAGCCCAGCCCAGCTGCGTGGCGCCCGGGACTCCGGGTCTCCTCATAAGCGGCCAGGGCAG
  CAGGACAACAGTTCTCCAGCCAAAACTGTAGGGCGCTTTTCCGTGGTCAGCACACAGGACGAGTGGACTC
  TGGCGTCACCTCACAGCCTGCGGTACTCTGCCCCACCCGATGTCTACCTGGATGAGATCCCCTCTAGCCC
  TGAGGTGAAGCTGGCAGTGCGGCGGGTACAGACAGCCTCCTCCATCGAGGTTGGCGTTGAGGAACCTGCC
  TCTAGTGACTCTGGGGATGAACGCCCAAGAAGGAGGTCCCAGGTGCAGAAGCAGTCCTCCCTGCCGGGTA
  CTGGTGGTGTGGCAAGTGACTTTGTGAAGAAGGCCACTGCCTTCCTGCACAGGTCTTCAAGGGCTGGCTC
  GCTGGGACCTGAGACACCCAGTAGGGCAGGTGTGAAGGTCCCTACCATCAGCATCACCTCCTTCCACTCC
  CAGTCATCCTACATCAGCAGTGACAATGACTCAGAGTTTGAGGATGCAGACATAAAGAAGGAACTGCGCA
  GTCTGCGAGAAAAGCACCTGAAGGAAATCTCGGAGCTACAGAGCCAGCAGAAGCAGGAGATCGAAGCCCT
  GTACCGACGCTTGGGCAAGCCGCTCCCTCCCAATGTGGGGTTCTTCCACACTGCACCCCCTATGGGCCGC
  AGGAGGAAAACCAGCAAGAGCAAATTGAAGGCTGGCAAGCTGCTGAACCCCTTGGTGCAACAGCTCAAGG
  TCGTGGCCTCCAGCACAGGTCATTTGTCTGACTCCAGCAGAGGCCCTCCCACTAAGGACCCTGCCCACGC
  CAGCACGCCCCCGGGCACCAAGGCAGTTCAGACCCAGCAGCCCTGCTCCGTTCGGGCCTCTCTGTCCACA
  GACATCTGCTCCGGTTTAGCCAGTGATGGAGGCGGAGCGCGTGGCCAAGGCTGGACGGTTTACCACCCAA
  CGTCTGAGAGAGGGGCCTATAAGTCTAGTAGCAAGCCTCGTGCTCGATTCCTCAGTGGACCCGTATCTGT
  GTCCATCTGGTCAGCCCTGAAGCGTCTCTGCCTAGGCAAAGAACACAGCAGTAGGTCTTCCACCAGCAGC
  CTGGCCCCAGGCCCTGAGCCAGGTCCCCAGCCCACCCTGCACGTCCAGGCGCAGGTGAACAACAGCAACA
  ACAAGAAAGGCACCTTCACGGACGACCTGCACAAGTTGGTGGACGAGTGGACAACGAAGACCGTGGGGGC
  AGCGCAAGTGAAGCCCACGCTCAACCAGCTGAAGCAGACGCAGAAGCTGCACGACATGGAGGCCTCTGGA
  GACGCTCGGGCTACATCTGTGCCTCGAGCAGCAGTGGGGGCATCGTGTCTGGCCCCGGCCCCTGGCCCTC
  TGTCCACCACAGCCACTCCTGGAGCCACTCCAGCCCTGCCTGTGCCCATACCAGGTACTGTCCACTCCAG
  CCTCTCAGGCCCTCCTTGTACACTGCCTCTATGTCAGTATGGAGGCCTGCTCCCTGATCCTGTGTCCTGG
  GGACCCTGGGTGGCCTCAGGAAACCCAGAAGGGTCTTGGGGCAGTCAGAGCCCCACTCAGGTCCCTGTGT
  TCCCAGTGTTTCTACGGCCACCTGTTATCAGCACACCAGGCCCCCGGCTGCACATCACG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231932 representing NM_001290311
  Red=Cloning site Green=Tags(s)
  
  MDGDGGRRDAPGALMEAGRGTGSAGMAEPRARAARLGPQRFLRRSVVESDQEEPPGLEAAETPSAQPPQP
  LQRRVLLLCKTRRLIAERARGRPAAPAPAAPAAPPGSPSVPSDPGPERAGTQEPSPDPTTASAAAAQVPD
  GGPRQEEAPAPTQEDAGTTEAKPEPGRARKDEPEEEEDDEDDLKAVATSLDGRFLKFDIELGRGSFKTVY
  KGLDTETWVEVAWCELQDRKLTKLERQRFKEEAEMLKGLQHPNIVRFYDFWESSAKGKRCIVLVTELMTS
  GTLKTYLKRFKVMKPKVLRSWCRQILKGLLFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKR
  ASFAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTCGIKPASFEK
  VHDPEIKEIIGECICKNKEERYEIKDLLSHAFFAEDTGVRVELAEEDHGRKSTIALRLWVEDPKKLKGKP
  KDNGAIEFTFDLEKETPDEVAQEMIDSGFFHESDVKIVAKSIRDRVALIQWRRERIWPALQSQEPKDSGS
  PDKARGLPAPLQVQVTYHAQSGQPGQPEPEEPEADQHLLPPTLPASVTSLASDSTFDSGQGSTVYSDSQS
  SQQSMVLSSLVDTAPTPASCVCSPPVSEGPGLTHSLPTLGAFQQPATVPGLSVGPVPPPARPPLLQQHFP
  ESSMSFTPVLPPPSTPVPTGPSQPAPPVQQPLPMAQPPTLPQVLAPQPMGTVQPVPSHLPPYLAPTSQVV
  APAQLKPLQMPQPPLQPLAQVPPQMPQMPVVPPITPLTGLDGLPQTLTDLPAANVAPVPPPQYFSPAVIL
  PSLTTPLPTSPALPMQAVKLPHPPGTPLAVPCQTIVPNAPAAIPLLAVAPQGVAALSIHPAVAQIPAQPV
  YPAAFPQMVPGDIPPSPHHTVQSLRATPPQLASPVPPQPVQPSVIHLPEQAAPTAASGTQVLLGHPPSYT
  ADVAAPVSAVSLPPAVLSPPLPDTLLPTVPDLLPKVPSSLAPTVVAASQSAPAQTSSLLLPTNPPLPTGP
  AVAGPCPAVQLMVEVAQEEQVSQDKPPGPPQSSESFGGSDVTSGRDLSDSCEGTFGGGRLEGRTARKHHR
  RSTRARSRQERASRPRLTILNVCNTGDKMVECQLETHNHKMVTFKFDLDGDAPDEIATYMVEHDFILPAE
  RETFIEQMKDVMDKAEDMLSEDTDADHGSDTGTSPPHLGTCGLATGEENRQSQANAPVYQQNVLHTGKRW
  FIICPVAEHPATDTSESSPPLPLSSLQPEASQDPAPYPDQLSLTDKPSFPAAQQLLSQAGSSNPPGGASA
  PLAPSSPPVTTVIPAAPATSTVPESAAGTAMQAGGPGTHQGPASVHETLQPLAETRSAQCTAQPLSTGQG
  PCTPALEASRCSTGLGEPISTREVSTQGEPLPASVPEPSPPTGATQSVPGQPPPPLPITVGAISLAAPQL
  PSPPLGPTAPPPPPSALESDGEGPPPRVGFVDNTIKSLDEKLRTLLYQEHVPTSSASAGTPMEASDRDFT
  LEPLRGDLPSALSDKTPSLTQQTQPSLEKSETAPAGWALAQREQGASSPMTAESSSSNTLGCDSDAGQVA
  SDSSTAPSVPQDASGSSVPTHMDPKDQNSSVPREALAAPMQSGPGSFTVGSPAQLRGARDSGSPHKRPGQ
  QDNSSPAKTVGRFSVVSTQDEWTLASPHSLRYSAPPDVYLDEIPSSPEVKLAVRRVQTASSIEVGVEEPA
  SSDSGDERPRRRSQVQKQSSLPGTGGVASDFVKKATAFLHRSSRAGSLGPETPSRAGVKVPTISITSFHS
  QSSYISSDNDSEFEDADIKKELRSLREKHLKEISELQSQQKQEIEALYRRLGKPLPPNVGFFHTAPPMGR
  RRKTSKSKLKAGKLLNPLVQQLKVVASSTGHLSDSSRGPPTKDPAHASTPPGTKAVQTQQPCSVRASLST
  DICSGLASDGGGARGQGWTVYHPTSERGAYKSSSKPRARFLSGPVSVSIWSALKRLCLGKEHSSRSSTSS
  LAPGPEPGPQPTLHVQAQVNNSNNKKGTFTDDLHKLVDEWTTKTVGAAQVKPTLNQLKQTQKLHDMEASG
  DARATSVPRAAVGASCLAPAPGPLSTTATPGATPALPVPIPGTVHSSLSGPPCTLPLCQYGGLLPDPVSW
  GPWVASGNPEGSWGSQSPTQVPVFPVFLRPPVISTPGPRLHIT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001290311
• ORF Size: 6639 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001290311.1, NP_001277240.1
• RefSeq Size: 8496
• RefSeq ORF: 6642
• Locus ID: 75607
• MW: 234.5 kDa