Cacna1a (NM_001252059) Mouse Tagged ORF Clone

CAT#: MR231941

Cacna1a (myc-DDK-tagged) - Mouse calcium channel, voltage-dependent, P/Q type, alpha 1A subunit (Cacna1a), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cacna1a
• Synonyms: alpha1A; APCA; BI; Caca1a; Cacnl1a4; Cav2.1; Ccha1a; EA2; FHM; HPCA; la; MHP; MHP1; nmf352; rkr; SCA6; smrl; tg
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231941 representing NM_001252059
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCGCTTTGGAGACGAGATGCCGGGCCGCTACGGCGCAGGCGGAGGAGGCTCAGGGCCGGCCGCCG
  GGGTGGTCGTGGGCGCCGCGGGCGGCCGAGGAGCCGGGGGCAGCCGGCAGGGCGGGCAGCCCGGAGCGCA
  GAGGATGTACAAGCAGTCGATGGCGCAGAGAGCGCGGACCATGGCCCTCTACAACCCCATCCCTGTCCGC
  CAGAACTGCCTCACGGTCAACCGCTCCCTGTTCCTCTTCAGTGAAGACAACGTGGATGACACGGAACCAT
  ACTTCATTGGAATCTTCTGTTTTGAGGCTGGAATTAAGATCGTGGCTCTTGGCTTTGCCTTCCACAAAGG
  CTCCTACCTGAGGAATGGCTGGAACGTCATGGACTTTGTCGTGGTGCTAACAGGCATCTTGGCCACTGTC
  GGGACGGAGTTTGACCTACGGACACTGAGGGCGGTTCGTGTGCTGCGGCCACTCAAGCTGGTGTCTGGAA
  TCCCAAGTTTACAAGTCGTCCTGAAGTCAATCATGAAGGCGATGATCCCTCTGCTGCAGATCGGCCTCCT
  CCTGTTTTTTGCAATCCTTATTTTTGCAATCATAGGGTTAGAATTTTATATGGGGAAATTTCATACCACC
  TGCTTTGAAGAGGGGACAGACGACATCCAGGGTGAGTCGCCAGCTCCGTGTGGGACAGAGGAGCCTGCCC
  GCACCTGCCCCAACGGGACCAAATGTCAGCCGTACTGGGAAGGGCCCAACAACGGCATCACTCAGTTCGA
  CAACATCCTGTTTGCTGTGCTCACTGTTTTCCAGTGCATCACCATGGAAGGCTGGACTGATCTCCTCTAC
  AATAGCAACGATGCCTCAGGGAACACTTGGAACTGGTTGTACTTCATCCCCCTCATCATCATCGGCTCCT
  TTTTTATGCTGAACCTTGTTCTGGGTGTGCTGTCTGGGGAGTTTGCCAAAGAAAGGGAACGTGTGGAGAA
  CCGGAGAGCTTTTCTGAAGCTCAGAAGACAACAGCAGATTGAACGTGAGCTCAATGGATACATGGAGTGG
  ATCTCAAAAGCAGAAGAGGTGATTCTTGCGGAGGACGAGACAGACGTGGAGCAGAGGCACCCTTTTGATG
  GAGCTCTTCGGAGAGCTACTCTGAAGAAAAGCAAGACAGACCTGCTCAACCCTGAGGAGGCCGAGGACCA
  GCTGGCTGACATCGCGTCTGTGGGGTCTCCCTTCGCCAGAGCCAGCATCAAAAGTGCCAAGCTGGAGAAC
  TCGACTTTTTTTCACAAGAAGGAGAGAAGAATGCGTTTCTACATCCGCCGCATGGTCAAAACTCAGGCCT
  TCTACTGGACCGTGCTCAGTCTGGTAGCCCTCAACACGCTGTGCGTCGCCATTGTCCACTACAACCAGCC
  CGAGTGGCTCTCCGACTTCCTCTACTATGCAGAATTCATTTTCTTAGGACTCTTTATGTCCGAAATGTTT
  ATAAAAATGTACGGGCTCGGGACGCGGCCTTACTTCCACTCTTCCTTCAACTGCTTTGACTGTGGGGTCA
  TCATTGGGAGCATCTTTGAAGTCATCTGGGCTGTCATCAAACCTGGTACATCCTTTGGAATCAGTGTGTT
  ACGAGCTCTCAGGTTATTGCGAATTTTCAAAGTCACAAAGTACTGGGCATCTCTCAGAAACCTGGTTGTC
  TCCCTCCTCAACTCCATGAAGTCCATCATAAGTCTGTTGTTCCTCCTTTTCCTGTTCATCGTTGTCTTTG
  CCCTCCTGGGGATGCAGCTGTTCGGTGGCCAATTTAATTTTGATGAGGGGACTCCGCCCACCAACTTCGA
  CACTTTTCCAGCAGCAATAATGACTGTGTTTCAGATCCTGACTGGCGAGGATTGGAATGAGGTCATGTAT
  GATGGGATCAAGTCTCAGGGGGGCGTGCAGGGCGGCATGGTGTTCTCCATCTACTTCATCGTCCTCACCC
  TCTTCGGGAACTACACCCTGCTGAACGTGTTCTTGGCCATCGCAGTGGACAACCTGGCCAATGCCCAGGA
  GCTCACCAAGGATGAGCAAGAAGAGGAAGAGGCAGCCAATCAGAAGCTGGCTCTACAGAAAGCCAAGGAG
  GTAGCAGAAGTGAGTCCCCTGTCTGCAGCCAACATGTCCATAGCCGTGAAGGAGCAGCAGAAGAACCAGA
  AGCCTACCAAGTCGGTGTGGGAGCAGCGCACCAGCGAGATGCGCAAGCAGAACCTGCTGGCCAGCCGCGA
  GGCGCTGTACGGGGACGCGGCTGAGCGCTGGCCCACCCCCTACGCGCGCCCGCTGCGGCCCGACGTGAAG
  ACTCACTTGGACCGGCCGCTTGTGGTGGACCCGCAGGAGAACCGTAACAACAACACCAACAAGAGCCGTG
  CGCCAGAAGCGCTGCGCCCGACCGCGCGGCCCCGCGAAAGCGCGCGCGACCCCGACGCGCGGCGCGCCTG
  GCCCGGCAGCCCTGAGCGCGCCCCTGGACGAGAGGGCCCGTATGGGCGCGAGAGCGAGCCTCAGCAGCGC
  GAGCACGCGCCGCCCCGCGAGCACGCACCCTGGGACGCGGACACCGAGCGCGCCAAAGCCGGGGACGCGC
  CCCGCCGCCACACGCACCGACCTGTGGCGGAGGGCGAGCCTCGCCGCCACCGCGCCCGCCGCCGGCCCGG
  GGACGAACCCGACGACAGGCCAGAGCGCAGGCCACGTCCCCGCGACGCCACTAGGCCGGCCCGCGCTGCA
  GACGGCGAAGGCGACGATGGGGAGCGCAAGCGGCGACACCGACACGGGCCGCCGGCCCACGACGACAGGG
  AGCGCAGACACCGACGCAGAAAAGAGAACCAGGGCTCTGGGGTCCCCGTGTCTGGTCCCAACTTGTCCAC
  CACCAGGCCAATCCAGCAGGACCTGGGCCGCCAGGACCTGCCGCTGGCTGAGGATCTTGACAACATGAAG
  AATAACAAGCTGGCCACCGGGGAACCTGCCAGTCCCCACGACAGCCTGGGCCACAGCGGCCTTCCCCCCA
  GCCCCGCCAAAATCGGGAACAGCACCAACCCTGGTCCCGCCTTGGCCACCAATCCCCAGAACGCTGCCAG
  CCGCAGGACGCCCAACAATCCGGGCAACCCGTCCAACCCCGGCCCCCCCAAGACTCCCGAGAACAGCCTT
  ATCGTCACCAACCCCAGCAGCACCCAGCCCAACTCAGCGAAGACTGCCAGGAAACCCGAGCACATGGCGG
  TGGAGATCCCCCCAGCCTGCCCGCCCCTCAACCACACCGTGGTCCAAGTAAACAAAAACGCCAACCCAGA
  CCCACTGCCAAAGAAAGAGGAAGAGAAGAAAGAGGAAGAGGAGGCAGACCCCGGGGAGGACGGCCCAAAG
  CCCATGCCGCCCTACAGCTCCATGTTCATCCTCTCCACCACCAACCCCCTTCGCCGGCTGTGCCATTACA
  TCCTGAACCTGCGCTACTTCGAGATGTGCATCCTCATGGTCATTGCCATGAGCAGCATCGCTCTGGCCGC
  CGAGGACCCGGTGCAGCCCAACGCACCCCGCAACAATGTGCTGCGATATTTTGACTATGTTTTTACAGGC
  GTGTTTACCTTTGAGATGGTGATCAAGATGATCGACCTGGGCCTCGTCTTGCATCAGGGGGCCTATTTCC
  GTGACCTATGGAACATTCTGGACTTCATAGTGGTCAGTGGGGCCCTGGTGGCCTTTGCCTTCACTGGCAA
  TAGCAAAGGAAAGGACATTAACACCATTAAGTCTCTCCGAGTCCTCCGGGTGCTACGACCTCTAAAGACC
  ATCAAGCGGCTGCCAAAGCTGAAGGCCGTGTTTGACTGCGTGGTGAACTCGCTCAAGAACGTCTTCAACA
  TCCTCATTGTGTACATGCTGTTCATGTTCATCTTCGCTGTGGTAGCCGTGCAGCTCTTCAAGGGCAAGTT
  CTTCCACTGCACGGACGAGTCCAAGGAGTTCGAGAGAGACTGTCGAGGCAAGTACCTCCTGTACGAGAAG
  AACGAAGTGAAGGCCCGGGACCGCGAGTGGAAGAAGTACGAGTTCCACTACGACAACGTGCTCTGGGCTC
  TGCTCACACTTTTCACAGTGTCCACAGGAGAGGGCTGGCCACAGGTCCTCAAGCACTCAGTGGATGCTAC
  TTTTGAGAACCAGGGCCCCAGCCCCGGGTACCGCATGGAGATGTCCATCTTCTACGTGGTGTACTTTGTA
  GTGTTTCCCTTCTTCTTTGTCAATATCTTCGTGGCCTTGATCATCATCACCTTTCAGGAGCAGGGAGACA
  AGATGATGGAAGAGTACAGCCTGGAGAAAAATGAGAGGGCCTGCATCGACTTTGCCATCAGTGCCAAGCC
  GCTGACCAGGCACATGCCCCAGAACAAGCAGAGCTTCCAGTATCGAATGTGGCAGTTCGTCGTGTCCCCA
  CCCTTTGAGTACACCATCATGGCCATGATCGCTCTCAACACCATCGTGCTAATGATGAAGTTCTACGGAG
  CCTCGGTGGCCTATGAAAACGCCCTTCGCGTGTTCAACATTGTCTTCACCTCCCTCTTCTCTCTCGAATG
  TGTGCTCAAAGTCATGGCTTTTGGGATTCTGAATTATTTCCGCGATGCCTGGAACATCTTCGACTTTGTG
  ACTGTTCTGGGCAGCATCACAGACATCCTCGTCACCGAGTTTGGGAATAACTTCATCAACCTGAGCTTTC
  TCCGCCTCTTCCGTGCTGCCAGACTCATCAAACTCCTCCGCCAGGGTTACACCATCCGCATTCTCCTCTG
  GACTTTCGTGCAGTCCTTCAAGGCCCTACCTTATGTCTGTCTGCTGATCGCCATGCTCTTCTTCATCTAC
  GCCATCATCGGGATGCAGGTGTTTGGCAACATCGGCATTGACGGGGAAGACGAGGACAGCGACGAGGATG
  AATTCCAAATCACGGAGCACAATAACTTCCGGACCTTCTTCCAAGCGCTCATGCTTCTCTTCCGGAGCGC
  CACAGGAGAAGCGTGGCACAACATCATGCTCTCCTGCCTCAGCGGGAAGCCGTGCGATAAGAACTCTGGG
  ATCCTCACGGCAGACTGCGGCAACGAGTTCGCGTACTTCTACTTTGTCTCGTTCATCTTCCTGTGCTCGT
  TTCTGATGCTGAATCTCTTTGTCGCTGTCATCATGGACAACTTCGAGTACCTCACCCGAGATTCTTCCAT
  CCTGGGCCCCCATCACTTGGATGAATACGTGCGTGTCTGGGCAGAGTATGACCCTGCTGCCTGCGGCCGC
  ATTCACTATAAGGACATGTACAGTTTATTGCGAGTAATATCGCCCCCTCTCGGCTTAGGCAAGAAATGTC
  CTCATAGGGTTGCTTGCAAGAGACTCCTGCGGATGGACCTGCCGGTAGCAGATGACAACACAGTTCACTT
  CAACTCCACCCTGATGGCTCTGATCCGAACCGCCCTTGATATCAAAATTGCCAAGGGTGGAGCTGACAAG
  CAGCAGATGGATGCAGAGCTTCGCAAGGAGATGATGGCCATTTGGCCCAACCTGTCTCAGAAGACCCTGG
  ATCTGCTGGTCACACCTCACAAGTCCACGGACCTGACAGTGGGTAAGATCTACGCAGCCATGATGATCAT
  GGAGTATTACCGGCAGAGCAAGGCCAAGAAACTGCAGGCCATGCGAGAGGAGCAGAACCGGACACCACTC
  ATGTTCCAGCGCATGGAGCCTCCATCACCAACACAGGAGGGAGGACCCAGCCAGAATGCCCTTCCCTCCA
  CTCAGCTGGACCCAGGAGGAGGCCTGATGGCTCACGAAGGCGGCATGAAGGAGAGCCCGTCCTGGGTGAC
  CCAGCGGGCCCAAGAGATGTTCCAGAAGACTGGCACCTGGAGCCCAGAGCGAGGGCCACCCATCGACATG
  CCTAACAGCCAGCCCAACTCCCAGTCTGTGGAGATGCGAGAAATGGGTACTGATGGCTACTCAGACAGCG
  AACACTACCTCCCCATGGAAGGCCAGACCAGGGCCGCCTCCATGCCCCGCCTCCCAGCAGAGAACCAGAG
  GAGAAGGGGCCGGCCACGTGGAAATGACCTCAGTACCATCTCTGATACCAGCCCCATGAAGCGCTCAGCC
  TCTGTGCTGGGACCCAAAGCCCGGCGACTGGATGACTACTCACTAGAGCGGGTACCGCCTGAGGAGAACC
  AACGGTACCACCAACGTCGCCGGGACCGTGGCCACCGCACCTCTGAGCGCTCTCTGGGCCGATACACTGA
  TGTGGACACAGGTTTGGGGACAGATCTGAGCATGACCACCCAATCGGGTGACCTGCCCTCCAAAGATCGG
  GACCAGGACCGGGGCCGACCCAAGGACCGGAAGCATCGGCCACACCACCACCACCACCACCATCATCATC
  ATCCCCCGGCCCCCGACCGGGATCGCTACGCACAGGAGCGGCCGGACACCGGCCGGGCGCGGGCCCGGGA
  GCAGCGCTGGTCCCGCTCGCCCAGCGAAGGTCGGGAGCACACGACACACAGACAGGGCAGTAGTTCCGTC
  AGTGGAAGCCCAGCCCCCTCTACGTCCGGCACCAGCACGCCGCGGCGCGGCCGCCGCCAGCTCCCCCAGA
  CCCCCTGCACCCCGCGGCCGCTTGTGTCCTACTCGCCCGCTCCGCGCAGGCCTGCGGCGCGCAGGATGGC
  GGGTCCCGCAGCGCCCCCTGGCGGCTCGCCGCGGGGTTGCCGCCGTGCGCCGCGCTGGCCTGCGCATGCG
  CCTGAGGGCCCGCGGCCGCGCGGTGCCGACTACACCGAGCCCGACAGCCCGCGCGAGCCGCCAGGGGGCG
  CGCACGATCCCGCGCCGCGGTCGCCCAGGACTCCGCGCGCCGCGGGCTGCGCGTCACCGCGCCACGGCCG
  CAGGCTGCCCAATGGTTACTACGCGGGGCACGGCGCGCCGCGGCCGCGCACAGCCCGCAGGGGCGCGCAC
  GACGCGTACAGCGAGAGCGAGGACGACTGGTGC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231941 representing NM_001252059
  Red=Cloning site Green=Tags(s)
  
  MARFGDEMPGRYGAGGGGSGPAAGVVVGAAGGRGAGGSRQGGQPGAQRMYKQSMAQRARTMALYNPIPVR
  QNCLTVNRSLFLFSEDNVDDTEPYFIGIFCFEAGIKIVALGFAFHKGSYLRNGWNVMDFVVVLTGILATV
  GTEFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILIFAIIGLEFYMGKFHTT
  CFEEGTDDIQGESPAPCGTEEPARTCPNGTKCQPYWEGPNNGITQFDNILFAVLTVFQCITMEGWTDLLY
  NSNDASGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYMEW
  ISKAEEVILAEDETDVEQRHPFDGALRRATLKKSKTDLLNPEEAEDQLADIASVGSPFARASIKSAKLEN
  STFFHKKERRMRFYIRRMVKTQAFYWTVLSLVALNTLCVAIVHYNQPEWLSDFLYYAEFIFLGLFMSEMF
  IKMYGLGTRPYFHSSFNCFDCGVIIGSIFEVIWAVIKPGTSFGISVLRALRLLRIFKVTKYWASLRNLVV
  SLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFDEGTPPTNFDTFPAAIMTVFQILTGEDWNEVMY
  DGIKSQGGVQGGMVFSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAANQKLALQKAKE
  VAEVSPLSAANMSIAVKEQQKNQKPTKSVWEQRTSEMRKQNLLASREALYGDAAERWPTPYARPLRPDVK
  THLDRPLVVDPQENRNNNTNKSRAPEALRPTARPRESARDPDARRAWPGSPERAPGREGPYGRESEPQQR
  EHAPPREHAPWDADTERAKAGDAPRRHTHRPVAEGEPRRHRARRRPGDEPDDRPERRPRPRDATRPARAA
  DGEGDDGERKRRHRHGPPAHDDRERRHRRRKENQGSGVPVSGPNLSTTRPIQQDLGRQDLPLAEDLDNMK
  NNKLATGEPASPHDSLGHSGLPPSPAKIGNSTNPGPALATNPQNAASRRTPNNPGNPSNPGPPKTPENSL
  IVTNPSSTQPNSAKTARKPEHMAVEIPPACPPLNHTVVQVNKNANPDPLPKKEEEKKEEEEADPGEDGPK
  PMPPYSSMFILSTTNPLRRLCHYILNLRYFEMCILMVIAMSSIALAAEDPVQPNAPRNNVLRYFDYVFTG
  VFTFEMVIKMIDLGLVLHQGAYFRDLWNILDFIVVSGALVAFAFTGNSKGKDINTIKSLRVLRVLRPLKT
  IKRLPKLKAVFDCVVNSLKNVFNILIVYMLFMFIFAVVAVQLFKGKFFHCTDESKEFERDCRGKYLLYEK
  NEVKARDREWKKYEFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATFENQGPSPGYRMEMSIFYVVYFV
  VFPFFFVNIFVALIIITFQEQGDKMMEEYSLEKNERACIDFAISAKPLTRHMPQNKQSFQYRMWQFVVSP
  PFEYTIMAMIALNTIVLMMKFYGASVAYENALRVFNIVFTSLFSLECVLKVMAFGILNYFRDAWNIFDFV
  TVLGSITDILVTEFGNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIY
  AIIGMQVFGNIGIDGEDEDSDEDEFQITEHNNFRTFFQALMLLFRSATGEAWHNIMLSCLSGKPCDKNSG
  ILTADCGNEFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRVWAEYDPAACGR
  IHYKDMYSLLRVISPPLGLGKKCPHRVACKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGADK
  QQMDAELRKEMMAIWPNLSQKTLDLLVTPHKSTDLTVGKIYAAMMIMEYYRQSKAKKLQAMREEQNRTPL
  MFQRMEPPSPTQEGGPSQNALPSTQLDPGGGLMAHEGGMKESPSWVTQRAQEMFQKTGTWSPERGPPIDM
  PNSQPNSQSVEMREMGTDGYSDSEHYLPMEGQTRAASMPRLPAENQRRRGRPRGNDLSTISDTSPMKRSA
  SVLGPKARRLDDYSLERVPPEENQRYHQRRRDRGHRTSERSLGRYTDVDTGLGTDLSMTTQSGDLPSKDR
  DQDRGRPKDRKHRPHHHHHHHHHHPPAPDRDRYAQERPDTGRARAREQRWSRSPSEGREHTTHRQGSSSV
  SGSPAPSTSGTSTPRRGRRQLPQTPCTPRPLVSYSPAPRRPAARRMAGPAAPPGGSPRGCRRAPRWPAHA
  PEGPRPRGADYTEPDSPREPPGGAHDPAPRSPRTPRAAGCASPRHGRRLPNGYYAGHGAPRPRTARRGAH
  DAYSESEDDWC
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001252059
• ORF Size: 6963 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001252059.1, NP_001238988.1
• RefSeq Size: 7788
• RefSeq ORF: 6966
• Locus ID: 12286
• MW: 262.6 kDa