Cnot1 (NM_001205226) Mouse Tagged ORF Clone

CAT#: MR231947

Cnot1 (myc-DDK-tagged) - Mouse CCR4-NOT transcription complex, subunit 1 (Cnot1), transcript variant 3

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cnot1
• Synonyms: 6030411K04Rik; AA815922; D830048B13
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231947 representing NM_001205226
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATCTTGACTCGCTCTCGCTGGCCTTGTCTCAAATCAGCTACCTGGTGGACAATTTAACCAAGAAAA
  ACTACCGAGCCAGCCAGCAGGAAATACAGCATATTGTGAATCGGCACGGTCCTGAGGCAGACAGGCATTT
  ATTACGCTGCCTATTTTCACATGTGGATTTCAGTGGCGATGGTAAAAGCAGTGGCAAAGATTTCCATCAG
  TTTCTGATTCAGGAGTGTGCCTCGCTGATTACAAAGCCAAATTTTATCTCAACATTGTCATATGCCATTG
  ATAATCCATTGCACTACCAGAAGAGTTTAAAGCCTGCACCCCACTTATTTGCCCAGCTGAGTAAAGTTCT
  CAAGCTAAGTAAAGTACAGGAGGTCATTTTTGGTCTTGCCCTTTTGAATTCTTCTAGCCCAGATCTCAGG
  GGTTTTGCTGCCCAGTTTATTAAACAGAAGCTTCCGGATCTTCTGCGTTCTTACATTGACGCAGACGTCA
  GTGGAAATCAAGAAGGTGGCTTCCAAGATATAGCAATAGAGGTCCTACACCTCCTACTCTCCCATCTCCT
  CTTTGGGCAGAAGGGAGCCTTTGGAGTTGGACAGGAACAGATAGACGCCTTTCTTAAGACACTACGCAGA
  GATTTTCCCCAAGAGCGCTGTCCTGTGGTGCTTGCACCACTTTTATACCCTGAAAAACGGGACATTCTAA
  TGGACAGGATTTTGCCTGATTCTGGAGGGGTAGCTAAAACCATGATGGAGAGCTCGCTAGCTGACTTCAT
  GCAGGAAGTGGGCTATGGCTTTTGTGCAAGTATTGAAGAATGTCGCAATATAATCATGCAGTTTGGTGTT
  CGGGAGGTCACAGCTGCCCAGGTTGCCAGGGTTTTGGGAATGATGGCTCGGACTCATTCAGGATTAACAG
  ACGGTATTCCATTACAGAGCATCTCTGCTCCTGGCAGTGGGATCTGGAGCGACGGAAAAGATAAAAGCGA
  GGGGGCACAGGCACACACGTGGAATGTCGAGGTTTTGATTGATGTTCTTAAAGAGCTGAACCCGAGTTTG
  AATTTCAAGGAAGTAACTTATGAACTGGACCATCCTGGATTTCAGATTCGTGACAGCAAAGGACTTCACA
  ATGTGGTTTATGGCATTCAAAGGGGTCTGGGCATGGAAGTGTTTCCAGTAGATTTTATATATAGGCCTTG
  GAAACATGCAGAAGGCCAGCTCTCCTTCATCCAGCATTCCCTGATAAACCCAGAGGTCTTCTGTTTTGCT
  GACTACCCCTGTCACACTGTTGCCACTGATATTTTGAAAGCACCACCAGAGGATGACAATCGAGAAATTG
  CCACATGGAAAAGCTTGGATTTGATTGAATCTCTGCTGAGGCTTGCAGAGGTTGGGCAGTATGAGCAAGT
  GAAACAACTCTTCAGCTTCCCTATCAAACACTGTCCAGACATGTTGGTATTGGCCTTACTTCAAATCAAC
  ACTTCCTGGCACACCCTACGCCATGAACTCATCTCTACTTTGATGCCAATTTTCCTTGGAAACCATCCTA
  ACTCTGCCATTATTTTGCACTATGCATGGCATGGGCAGGGGCAGTCTCCATCAATTCGCCAACTTATAAT
  GCATGCAATGGCAGAATGGTACATGAGAGGGGAGCAGTATGATCAGGCCAAATTGTCTCGAATACTTGAC
  GTGGCCCAGGACTTGAAGGCCCTGTCGATGCTGCTGAACGGCACTCCATTTGCTTTTGTCATTGACCTTG
  CTGCACTCGCTTCACGGCGGGAATACCTCAAACTTGACAAGTGGCTAACAGATAAGATAAGAGAGCATGG
  GGAGCCTTTTATACAAGCATGTATGACTTTTTTAAAGAGAAGGTGCCCTTCTATTTTGGGTGGACTTGCC
  CCAGAGAAAGATCAGCCCAAAAGTGCTCAACTTCCCGCTGAAACTCTGGCAACAATGTTGGCTTGTCTGC
  AGGCTTGTGCAGGGAGTGTTTCTCAAGAGTTATCAGAGACCATCCTCACCATGGTAGCCAATTGCAGTAA
  TGTAATGAATAAGGCCAGACAGCCACCACCTGGAGTTATGCCAAAAGGTCGCCCTCCTAGTGCTAGCAGC
  TTAGATGCCATTTCTCCTGTTCAGATTGACCCTCTTGCTGGAATGGCATCTCTTAGTATAGGTGGTTCGG
  CTGCCCCTCACACCCAGAGCATGCAGGGTTTTCCTCCAAATCTGGGTTCTGCGTTCAGTACCCCTCAGTC
  ACCAGCAAAAGCATTTCCACCACTTTCAACCCCAAATCAAACAACGGCCTTCAGTGGGATTGGAGGACTC
  TCCTCACAGCTTCCAGTAGGTGGTCTTGGCACAGGAAGCCTGACTGGCATAGGAACTGGGGCCCTTGGAC
  TTCCTGCAGTGAACAATGATCCTTTTGTACAGAGGAAACTGGGCACCTCTGGACTCAACCAGCCTACATT
  CCAGCAGACCGACTTGTCTCAGGTGTGGCCAGAGGCAAATCAGCACTTTAGTAAAGAAATTGATGATGAA
  GCAAACAGTTATTTCCAGCGAATATATAATCACCCACCACATCCAACCATGTCTGTTGATGAGGTATTAG
  AAATGCTACAGAGATTTAAAGACTCTACAATAAAAAGGGAACGAGAAGTATTTAACTGTATGCTGAGGAA
  CTTGTTTGAAGAATACCGGTTTTTCCCTCAGTACCCTGATAAAGAATTACATATAACAGCTTGCTTGTTT
  GGTGGCATAATTGAAAAAGGACTGGTCACTTACATGGCATTGGGTCTGGCCCTGCGATATGTTCTTGAAG
  CCTTACGGAAGCCTTTTGGATCCAAAATGTATTATTTTGGTATTGCTGCACTAGATAGATTTAAAAATAG
  ATTGAAGGACTATCCTCAGTATTGTCAGCACCTGGCTTCTATCAGCCACTTTATGCAGTTCCCACATCAT
  TTGCAGGAGTACATTGAGTACGGACAGCAGTCTCGAGATCCTCCTGTGAAAATGCAGGGCTCTATTACTA
  CCCCTGGAAGTATTGCACTGGCTCAGGCCCAGGCTCAGGCCCAGGTTCCAGCAAAAGCTCCTCTTGCCGG
  TCAGGTTAACACAATGGTAACCACCTCAACAACCACCACTGTTGCTAAAACGGTTACAGTCACCAAGCCA
  ACGGGAGTCAGCTTTAAGAAAGATGTGCCACCTTCTATAAATACAACAAATATCGATACATTGCTTGTGG
  CCACAGATCAAACTGAGAGGATTGTGGAGCCCCCAGAAAATATTCAAGAGAAAATTGCTTTTATTTTCAA
  CAATCTCTCCCAGTCAAATATGACACAGAAGGTTGAAGAGCTTAAAGAAACTGTGAAGGAAGAATTTATG
  CCTTGGGTTTCCCAGTATCTGGTTATGAAAAGAGTCAGTATTGAGCCAAACTTTCATAGCCTGTATTCAA
  ACTTTCTTGATACATTGAAGAATCCTGAATTTAACAAGATGGTTCTGAATGAAACCTACAGAAACATTAA
  AGTGCTGCTGACTTCTGATAAAGCTGCGGCCAATTTCTCAGATCGTTCTTTGCTGAAGAACTTGGGACAT
  TGGTTAGGAATGATCACATTAGCTAAAAATAAACCCATCTTACACACAGACTTGGATGTGAAATCTTTGC
  TGTTAGAGGCTTACGTTAAAGGACAGCAAGAGTTGCTCTATGTGGTGCCCTTTGTTGCCAAAGTTCTAGA
  GTCCAGCATTCGCAGCCTGGTTTTTAGACCACCAAACCCCTGGACAATGGCAATTATGAATGTATTGGCT
  GAGCTGCATCAGGAGCATGACTTAAAGTTAAACCTGAAGTTTGAGATTGAGGTGCTCTGTAAGAACCTTG
  CATTAGACATCAATGAGCTCAAACCTGGAAACCTGCTGAAAGATAAAGATCGTCTGAAGAACTTAGATGA
  GCAACTCTCTGCTCCAAAGAAAGATGTCAAGCAGCCCGAAGAGCTTCCTGCCATCACAACAACAACAACT
  TCTACAACACCAGCTACCAGTACTACGTGTACAGCCACAGTTCCACCACAGCCACAGTACAGCTACCACG
  ACATCAATGTCTACTCTCTAGCAGGCCTGGCACCTCATATTACTCTAAACCCTACAATCCCTTTATTTCA
  AGCTCATCCTCAGCTGAAGCAGTGTGTGCGGCAAGCAATTGAAAGAGCTGTCCAGGAGCTTGTCCATCCT
  GTGGTGGATCGCTCAATTAAGATTGCTATGACTACGTGCGAACAGATAGTCAGAAAGGACTTTGCCCTGG
  ATTCTGAGGAGTCTCGAATGAGAATAGCAGCTCATCACATGATGCGGAACTTGACAGCTGGAATGGCTAT
  GATTACTTGCAGGGAACCTTTACTGATGAGCATATCCACCAACCTCAAAAACAGCTTTGCCTCAGCCCTG
  CGTACTGCATCCCCACAGCAAAGGGAAATGATGGACCAGGCTGCTGCTCAGTTAGCTCAGGACAATTGTG
  AATTAGCTTGTTGTTTTATTCAGAAAACTGCAGTGGAGAAAGCAGGCCCTGAGATGGACAAGAGATTGGC
  AACTGAATTTGAGCTGAGGAAGCATGCCCGGCAAGAAGGACGTAGGTACTGTGATCCTGTGGTCTTAACC
  TACCAAGCCGAGCGGATGCCGGAGCAGATCAGGCTGAAAGTTGGTGGTGTGGACCCAAAGCAGCTAGCCG
  TCTATGAAGAGTTTGCACGAAATGTGCCTGGCTTCTTACCTACAAATGACTTAAGTCAGCCTACAGGGTT
  TTTAGCTCAGCCCATGAAGCAAGCTTGGGCAACTGATGATGTAGCGCAGATTTATGATAAGTGTATTACA
  GAACTGGAGCAGCACCTACACGCCATTCCACCCACTCTGGCCATGAATCCTCAAGCCCAGGCTCTCCGAA
  GTCTTTTAGAGGTTGTAGTGTTGTCTCGGAATTCTCGGGATGCGATAGCTGCTCTTGGATTACTACAGAA
  GGCTGTAGAAGGCTTATTAGATGCCACAAGTGGTGCTGACGCCGACTTACTGCTGCGATACAGGGAGTGC
  CACCTGTTGGTACTGAAAGCTCTGCAGGATGGCAGGGCATATGGATCTCCATGGTGCAACAAACAAATCA
  CAAGGTGCCTAATTGAATGTCGAGATGAATATAAATATAATGTGGAGGCTGTGGAATTGTTAATTCGAAA
  TCATTTGGTTAACATGCAGCAGTATGATCTTCATCTTGCTCAGTCGATGGAGAATGGCTTGAACTATATG
  GCCGTGGCGTTTGCTATGCAACTGGTAAAAATTCTGCTGGTGGATGAAAGGAGTGTTGCCCATATTACTG
  AGGCAGATCTGTTCCATACCATTGAAACCCTCATGAGAATTAATGCTCATTCCAGAGGCAATGCTCCAGA
  AGGGTTGCCCCAGCTGATGGAAGTGGTGAGATCCAACTATGAAGCAATGATTGATCGTGCTCATGGAGGC
  CCTAACTTTATGATGCATTCTGGGATCTCTCAAGCCTCAGAATATGATGACCCTCCAGGCCTGAGGGAGA
  AGGCAGAGTATCTTCTGAGGGAATGGGTGAATCTCTACCATTCAGCAGCAGCTGGCCGCGACAGTACCAA
  AGCATTCTCTGCGTTTGTTGGACAGATGCACCAACAAGGAATACTTAAAACTGATGACCTCATAACAAGG
  TTTTTTCGTCTTTGCACTGAAATGTGTGTTGAAATCAGTTACCGTGCTCAGGCGGAGCAGCAGCACAATC
  CTGCGGCTAACCCTACCATGATCCGGGCCAAGTGCTACCACAACCTGGATGCCTTTGTCCGCCTCATTGC
  ACTGTTAGTGAAGCACTCTGGGGAGGCCACCAACACTGTCACAAAGATCAACCTGCTCAATAAGGTTCTC
  GGTATAGTAGTGGGAGTTCTCCTACAGGATCATGATGTTCGGCAGAGTGAATTTCAGCAACTTCCGTACC
  ATAGGATTTTTATCATGCTGCTGTTGGAGCTCAATGCACCCGAGCATGTGTTGGAAACCATTAATTTCCA
  GACTCTTACTGCTTTCTGCAATACATTCCACATCTTGAGACCTACTAAAGCTCCTGGCTTTGTATATGCT
  TGGCTCGAATTGATTTCCCATCGGATCTTTATTGCAAGAATGCTGGCACATACGCCACAGCAGAAGGGGT
  GGCCTATGTATGCTCAGCTGTTGATTGATTTATTCAAATATTTAGCACCTTTCCTTAGAAATGTGGAACT
  CACTAAACCTATGCAAATCCTCTACAAGGGTACTCTGAGAGTGCTGCTGGTTCTTTTGCATGATTTCCCA
  GAGTTCCTTTGTGATTACCATTATGGGTTCTGTGATGTGATCCCTCCTAATTGTATCCAGTTAAGAAATT
  TGATCCTGAGTGCCTTCCCAAGAAACATGAGGCTCCCAGACCCATTCACTCCTAATCTGAAGGTGGACAT
  GTTAAGCGAAATTAACATCGCACCTCGGATCCTCACCAATTTCACTGGAGTGATGCCGCCTCAGTTCAAA
  AAGGATTTGGATTCTTATCTTAAAACTCGGTCGCCGGTCACTTTCCTGTCTGATCTGCGTAGTAACCTAC
  AGGTATCTAATGAGCCCGGGAATCGCTACAACCTCCAGCTTATCAATGCATTGGTGCTCTACGTAGGGAC
  TCAGGCTATCGCACACATCCACAACAAGGGCAGCACGCCCTCAATGAGCACCATCACCCACTCAGCACAC
  ATGGACATCTTCCAGAACCTGGCTGTGGACCTAGACACTGAGGGCCGATATCTCTTCTTAAATGCAATTG
  CGAACCAGCTACGGTACCCAAACAGCCACACTCACTACTTCAGCTGCACAATGTTGTACCTTTTTGCTGA
  GGCGAACACTGAGGCAATCCAGGAACAGATCACAAGGGTTCTCTTGGAACGGTTGATTGTAAATAGGCCA
  CATCCTTGGGGTCTTCTTATCACCTTCATTGAGCTGATTAAAAACCCAGCATTTAAGTTCTGGAACCATG
  AATTTGTACACTGTGCCCCAGAGATTGAAAAGTTATTTCAGTCTGTTGCACAGTGCTGCATGGGACAGAA
  GCAGGCTCAGCAAGTGATGGAGGGGACCGGTGCCAGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231947 representing NM_001205226
  Red=Cloning site Green=Tags(s)
  
  MNLDSLSLALSQISYLVDNLTKKNYRASQQEIQHIVNRHGPEADRHLLRCLFSHVDFSGDGKSSGKDFHQ
  FLIQECASLITKPNFISTLSYAIDNPLHYQKSLKPAPHLFAQLSKVLKLSKVQEVIFGLALLNSSSPDLR
  GFAAQFIKQKLPDLLRSYIDADVSGNQEGGFQDIAIEVLHLLLSHLLFGQKGAFGVGQEQIDAFLKTLRR
  DFPQERCPVVLAPLLYPEKRDILMDRILPDSGGVAKTMMESSLADFMQEVGYGFCASIEECRNIIMQFGV
  REVTAAQVARVLGMMARTHSGLTDGIPLQSISAPGSGIWSDGKDKSEGAQAHTWNVEVLIDVLKELNPSL
  NFKEVTYELDHPGFQIRDSKGLHNVVYGIQRGLGMEVFPVDFIYRPWKHAEGQLSFIQHSLINPEVFCFA
  DYPCHTVATDILKAPPEDDNREIATWKSLDLIESLLRLAEVGQYEQVKQLFSFPIKHCPDMLVLALLQIN
  TSWHTLRHELISTLMPIFLGNHPNSAIILHYAWHGQGQSPSIRQLIMHAMAEWYMRGEQYDQAKLSRILD
  VAQDLKALSMLLNGTPFAFVIDLAALASRREYLKLDKWLTDKIREHGEPFIQACMTFLKRRCPSILGGLA
  PEKDQPKSAQLPAETLATMLACLQACAGSVSQELSETILTMVANCSNVMNKARQPPPGVMPKGRPPSASS
  LDAISPVQIDPLAGMASLSIGGSAAPHTQSMQGFPPNLGSAFSTPQSPAKAFPPLSTPNQTTAFSGIGGL
  SSQLPVGGLGTGSLTGIGTGALGLPAVNNDPFVQRKLGTSGLNQPTFQQTDLSQVWPEANQHFSKEIDDE
  ANSYFQRIYNHPPHPTMSVDEVLEMLQRFKDSTIKREREVFNCMLRNLFEEYRFFPQYPDKELHITACLF
  GGIIEKGLVTYMALGLALRYVLEALRKPFGSKMYYFGIAALDRFKNRLKDYPQYCQHLASISHFMQFPHH
  LQEYIEYGQQSRDPPVKMQGSITTPGSIALAQAQAQAQVPAKAPLAGQVNTMVTTSTTTTVAKTVTVTKP
  TGVSFKKDVPPSINTTNIDTLLVATDQTERIVEPPENIQEKIAFIFNNLSQSNMTQKVEELKETVKEEFM
  PWVSQYLVMKRVSIEPNFHSLYSNFLDTLKNPEFNKMVLNETYRNIKVLLTSDKAAANFSDRSLLKNLGH
  WLGMITLAKNKPILHTDLDVKSLLLEAYVKGQQELLYVVPFVAKVLESSIRSLVFRPPNPWTMAIMNVLA
  ELHQEHDLKLNLKFEIEVLCKNLALDINELKPGNLLKDKDRLKNLDEQLSAPKKDVKQPEELPAITTTTT
  STTPATSTTCTATVPPQPQYSYHDINVYSLAGLAPHITLNPTIPLFQAHPQLKQCVRQAIERAVQELVHP
  VVDRSIKIAMTTCEQIVRKDFALDSEESRMRIAAHHMMRNLTAGMAMITCREPLLMSISTNLKNSFASAL
  RTASPQQREMMDQAAAQLAQDNCELACCFIQKTAVEKAGPEMDKRLATEFELRKHARQEGRRYCDPVVLT
  YQAERMPEQIRLKVGGVDPKQLAVYEEFARNVPGFLPTNDLSQPTGFLAQPMKQAWATDDVAQIYDKCIT
  ELEQHLHAIPPTLAMNPQAQALRSLLEVVVLSRNSRDAIAALGLLQKAVEGLLDATSGADADLLLRYREC
  HLLVLKALQDGRAYGSPWCNKQITRCLIECRDEYKYNVEAVELLIRNHLVNMQQYDLHLAQSMENGLNYM
  AVAFAMQLVKILLVDERSVAHITEADLFHTIETLMRINAHSRGNAPEGLPQLMEVVRSNYEAMIDRAHGG
  PNFMMHSGISQASEYDDPPGLREKAEYLLREWVNLYHSAAAGRDSTKAFSAFVGQMHQQGILKTDDLITR
  FFRLCTEMCVEISYRAQAEQQHNPAANPTMIRAKCYHNLDAFVRLIALLVKHSGEATNTVTKINLLNKVL
  GIVVGVLLQDHDVRQSEFQQLPYHRIFIMLLLELNAPEHVLETINFQTLTAFCNTFHILRPTKAPGFVYA
  WLELISHRIFIARMLAHTPQQKGWPMYAQLLIDLFKYLAPFLRNVELTKPMQILYKGTLRVLLVLLHDFP
  EFLCDYHYGFCDVIPPNCIQLRNLILSAFPRNMRLPDPFTPNLKVDMLSEINIAPRILTNFTGVMPPQFK
  KDLDSYLKTRSPVTFLSDLRSNLQVSNEPGNRYNLQLINALVLYVGTQAIAHIHNKGSTPSMSTITHSAH
  MDIFQNLAVDLDTEGRYLFLNAIANQLRYPNSHTHYFSCTMLYLFAEANTEAIQEQITRVLLERLIVNRP
  HPWGLLITFIELIKNPAFKFWNHEFVHCAPEIEKLFQSVAQCCMGQKQAQQVMEGTGAS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001205226
• ORF Size: 7107 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001205226.1, NP_001192155.1
• RefSeq Size: 8369
• RefSeq ORF: 7110
• Locus ID: 234594
• MW: 266.6 kDa