METTL9 (NM_016025) Human Tagged ORF Clone

CAT#: RC200028

METTL9 (Myc-DDK-tagged)-Human methyltransferase like 9 (METTL9), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY414244) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200028 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified METTL9 protein (Cat# TP300028). The protein was produced from HEK293T cells transfected with METTL9 cDNA clone (Cat# RC200028) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: METTL9
• Synonyms: CGI-81; DREV; DREV1; PAP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200028 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGACTGCTGGCGGGCTGGCTGTGCCTGAGCCTGGCGTCCGTGTGGCTGGCGCGGAGGATGTGGACGC
  TGCGGAGCCCGCTCACCCGCTCCCTGTACGTGAACATGACTAGCGGCCCGGGTGGGCCGGCGGCGGCCGC
  GGGCGGCAGGAAGGAGAACCACCAGTGGTATGTGTGCAACAGAGAGAAATTATGCGAATCACTCCAGGCT
  GTCTTTGTTCAGAGTTACCTTGATCAAGGAACACAGATCTTCTTAAACAACAGCATTGAGAAATCGGGCT
  GGCTATTTATCCAATTATATCATTCTTTTGTGTCATCTGTTTTTAGCCTGTTTATGTCTAGAACATCTAT
  CAATGGGTTGCTAGGAAGAGGCTCAATGTTTGTGTTTTCACCAGATCAGTTTCAGAGACTGCTTAAAATT
  AATCCAGACTGGAAAACCCACAGACTTCTTGATTTAGGTGCTGGAGATGGAGAAGTCACAAAAATCATGA
  GCCCTCATTTTGAAGAAATCTATGCCACTGAGCTTTCTGAAACTATGATATGGCAGCTTCAGAAAAAGAA
  ATACAGAGTCCTTGGTATAAATGAATGGCAGAATACGGGGTTCCAGTATGATGTCATCAGCTGCCTGAAC
  TTGCTGGACCGCTGTGATCAGCCCCTGACTTTGTTAAAAGATATCAGAAGTGTCTTGGAGCCAACTAGAG
  GCAGGGTCATCCTTGCCCTTGTCCTCCCCTTTCATCCCTATGTGGAAAACGTAGGTGGCAAGTGGGAGAA
  ACCATCAGAAATTTTGGAAATCAAAGGACAGAACTGGGAAGAACAAGTGAATAGTCTGCCTGAAGTTTTC
  AGAAAAGCTGGTTTTGTTATCGAAGCTTTCACCAGACTACCATACCTGTGTGAAGGCGACATGTATAATG
  ACTACTACGTTCTGGATGACGCTGTCTTTGTTCTCAAACCAGTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200028 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRLLAGWLCLSLASVWLARRMWTLRSPLTRSLYVNMTSGPGGPAAAAGGRKENHQWYVCNREKLCESLQA
  VFVQSYLDQGTQIFLNNSIEKSGWLFIQLYHSFVSSVFSLFMSRTSINGLLGRGSMFVFSPDQFQRLLKI
  NPDWKTHRLLDLGAGDGEVTKIMSPHFEEIYATELSETMIWQLQKKKYRVLGINEWQNTGFQYDVISCLN
  LLDRCDQPLTLLKDIRSVLEPTRGRVILALVLPFHPYVENVGGKWEKPSEILEIKGQNWEEQVNSLPEVF
  RKAGFVIEAFTRLPYLCEGDMYNDYYVLDDAVFVLKPV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016025
• ORF Size: 954 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016025.1, NM_016025.2, NP_057109.2
• RefSeq Size: 3267 bp
• RefSeq ORF: 957 bp
• Locus ID: 51108
• Domains: DREV
• MW: 36.5 kDa