MRPL37 (NM_016491) Human Tagged ORF Clone

CAT#: RC200096

MRPL37 (Myc-DDK-tagged)-Human mitochondrial ribosomal protein L37 (MRPL37), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MRPL37
• Synonyms: L2mt; L37mt; MRP-L2; MRP-L37; MRPL2; RPML2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200096 representing NM_016491
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCATTGGCGTCCGGGCCCGCAAGGCGGGCGCTAGCTGGCTCCGGGCAGCTCGGCCTTGGGGGCTTCG
  GGGCCCCGAGACGCGGGGCGTATGAGTGGGGCGTGCGCTCCACGCGGAAGTCGGAGCCTCCTCCCCTGGA
  TAGGGTGTACGAGATCCCTGGACTGGAGCCCATCACCTTTGCGGGGAAGATGCACTTCGTGCCCTGGCTG
  GCGCGGCCGATCTTTCCGCCCTGGGACCGCGGCTACAAGGACCCAAGGTTCTACCGCTCGCCCCCTCTTC
  ACGAGCATCCGCTGTACAAAGACCAGGCCTGCTATATCTTTCACCACCGTTGCCGCCTTCTCGAGGGTGT
  AAAGCAGGCCCTCTGGCTCACCAAGACCAAGTTAATAGAAGGCCTTCCCGAGAAAGTGCTTAGCCTTGTT
  GATGATCCAAGGAACCACATAGAGAACCAAGACGAGTGCGTTCTGAATGTGATCTCTCACGCCCGTCTCT
  GGCAGACCACTGAGGAAATCCCCAAGAGAGAGACCTACTGCCCGGTCATCGTGGACAACCTAATACAGCT
  GTGTAAATCTCAGATTCTCAAGCATCCTTCTCTGGCCAGGAGGATCTGTGTCCAAAACTCCACGTTTTCT
  GCTACCTGGAACCGAGAGTCTCTTCTCCTTCAAGTCCGTGGTTCTGGTGGAGCCCGACTGAGCACTAAGG
  ATCCTCTGCCCACCATCGCCTCCAGAGAGGAGATTGAAGCTACTAAGAATCATGTTCTAGAGACCTTCTA
  CCCCATATCACCCATCATCGATCTTCATGAATGCAATATTTATGATGTGAAAAATGACACAGGATTCCAG
  GAAGGCTATCCTTACCCCTATCCCCATACCCTGTACTTACTGGACAAAGCCAATTTACGACCACACCGCC
  TTCAACCAGATCAGCTGCGGGCCAAGATGATCCTGTTTGCTTTTGGCAGTGCCCTGGCTCAGGCCCGGCT
  CCTCTATGGGAATGATGCCAAGGTCTTGGAGCAGCCCGTGGTGGTGCAGAGCGTGGGCACGGATGGACGT
  GTCTTCCATTTCCTAGTGTTTCAACTGAATACCACAGACCTGGACTGTAACGAGGGTGTCAAGAATTTGG
  CCTGGGTGGACTCAGACCAGCTCCTCTATCAGCATTTTTGGTGTCTCCCAGTGATCAAAAAGAGAGTGGT
  TGTGGAACCTGTTGGCCCAGTTGGTTTCAAGCCAGAGACATTCAGAAAGTTTTTAGCTCTATATTTGCAT
  GGTGCTGCG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200096 representing NM_016491
  Red=Cloning site Green=Tags(s)
  
  MALASGPARRALAGSGQLGLGGFGAPRRGAYEWGVRSTRKSEPPPLDRVYEIPGLEPITFAGKMHFVPWL
  ARPIFPPWDRGYKDPRFYRSPPLHEHPLYKDQACYIFHHRCRLLEGVKQALWLTKTKLIEGLPEKVLSLV
  DDPRNHIENQDECVLNVISHARLWQTTEEIPKRETYCPVIVDNLIQLCKSQILKHPSLARRICVQNSTFS
  ATWNRESLLLQVRGSGGARLSTKDPLPTIASREEIEATKNHVLETFYPISPIIDLHECNIYDVKNDTGFQ
  EGYPYPYPHTLYLLDKANLRPHRLQPDQLRAKMILFAFGSALAQARLLYGNDAKVLEQPVVVQSVGTDGR
  VFHFLVFQLNTTDLDCNEGVKNLAWVDSDQLLYQHFWCLPVIKKRVVVEPVGPVGFKPETFRKFLALYLH
  GAA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016491
• ORF Size: 1269 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_016491.2, NM_016491.3, NP_057575.2
• RefSeq Size: 1487
• RefSeq ORF: 1272
• Locus ID: 51253
• MW: 48.1 kDa
• Gene Summary: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. [provided by RefSeq, Jul 2008]