N acetylglucosamine kinase (NAGK) (NM_017567) Human Tagged ORF Clone

CAT#: RC200113

NAGK (Myc-DDK-tagged)-Human N-acetylglucosamine kinase (NAGK)

Product Images

Western blot validation of overexpression lysate (Cat# LY413709) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200113 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified NAGK protein (Cat# TP300113). The protein was produced from HEK293T cells transfected with NAGK cDNA clone (Cat# RC200113) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NAGK
• Synonyms: GNK; HSA242910
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200113 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGCGATCTATGGGGGTGTAGAGGGGGGAGGCACACGATCCGAGGTCCTTTTAGTCTCAGAGGATG
  GGAAGATCCTGGCAGAAGCAGATGGACTGAGCACAAACCACTGGCTGATCGGGACAGACAAGTGTGTGGA
  GAGGATCAATGAGATGGTGAACAGGGCCAAACGGAAAGCAGGGGTGGATCCTCTGGTACCGCTGCGAAGC
  TTGGGCCTATCTCTGAGCGGTGGGGACCAGGAGGACGCGGGGAGGATCCTGATCGAGGAGCTCAGGGACC
  GATTTCCCTACCTGAGTGAAAGCTACTTAATCACCACCGATGCCGCCGGCTCCATCGCCACAGCTACACC
  GGATGGTGGAGTTGTGCTCATATCTGGAACAGGCTCCAACTGCAGGCTCATCAACCCTGATGGCTCCGAG
  AGTGGCTGCGGCGGCTGGGGCCATATGATGGGTGATGAGGGTTCAGCCTACTGGATCGCACACCAAGCAG
  TGAAAATAGTGTTTGACTCCATTGACAACCTAGAGGCGGCTCCTCATGATATCGGCTACGTCAAACAGGC
  CATGTTCCACTATTTCCAGGTGCCAGATCGGCTAGGGATACTCACTCACCTGTATAGGGACTTTGATAAA
  TGCAGGTTTGCTGGGTTTTGCCGGAAAATTGCAGAAGGTGCTCAGCAGGGAGACCCCCTTTCCCGCTATA
  TCTTCAGGAAGGCTGGGGAGATGCTGGGCAGACACATCGTAGCAGTGTTGCCCGAGATTGACCCGGTCTT
  GTTCCAGGGCAAGATTGGACTCCCCATCCTGTGCGTGGGCTCTGTGTGGAAGAGCTGGGAGCTGCTGAAG
  GAAGGTTTTCTTCTGGCGCTGACCCAGGGCAGAGAGATCCAGGCTCAGAACTTCTTCTCCAGCTTCACCC
  TGATGAAGCTGAGGCACTCCTCCGCTCTGGGTGGGGCCAGCCTAGGGGCCAGGCACATCGGGCACCTCCT
  CCCCATGGACTATAGCGCCAATGCCATTGCCTTCTATTCCTACACCTTTTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200113 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAIYGGVEGGGTRSEVLLVSEDGKILAEADGLSTNHWLIGTDKCVERINEMVNRAKRKAGVDPLVPLRS
  LGLSLSGGDQEDAGRILIEELRDRFPYLSESYLITTDAAGSIATATPDGGVVLISGTGSNCRLINPDGSE
  SGCGGWGHMMGDEGSAYWIAHQAVKIVFDSIDNLEAAPHDIGYVKQAMFHYFQVPDRLGILTHLYRDFDK
  CRFAGFCRKIAEGAQQGDPLSRYIFRKAGEMLGRHIVAVLPEIDPVLFQGKIGLPILCVGSVWKSWELLK
  EGFLLALTQGREIQAQNFFSSFTLMKLRHSSALGGASLGARHIGHLLPMDYSANAIAFYSYTFS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017567
• ORF Size: 1032 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017567.1, NM_017567.2, NM_017567.3, NP_060037.2
• RefSeq Size: 1801 bp
• RefSeq ORF: 1035 bp
• Locus ID: 55577
• Domains: BcrAD_BadFG
• Protein Families: Druggable Genome
• Protein Pathways: Amino sugar and nucleotide sugar metabolism
• MW: 37.4 kDa
• Gene Summary: This gene encodes a member of the N-acetylhexosamine kinase family. The encoded protein catalyzes the conversion of N-acetyl-D-glucosamine to N-acetyl-D-glucosamine 6-phosphate, and is the major mammalian enzyme which recovers amino sugars. [provided by RefSeq, Nov 2011]