NAD Synthetase (NADSYN1) (NM_018161) Human Tagged ORF Clone

CAT#: RC200131

NADSYN1 (Myc-DDK-tagged)-Human NAD synthetase 1 (NADSYN1)

Product Images

Western blot validation of overexpression lysate (Cat# LY413259) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200131 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified NADSYN1 protein (Cat# TP300131). The protein was produced from HEK293T cells transfected with NADSYN1 cDNA clone (Cat# RC200131) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NADSYN1
• Synonyms: FLJ10631; FLJ36703; FLJ40627
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200131 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCCGGAAGGTGACCGTGGCCACCTGCGCACTCAACCAGTGGGCCCTGGACTTCGAGGGCAATTTGC
  AAAGAATTTTAAAGAGTATTGAAATTGCCAAAAACAGAGGAGCAAGATACAGGCTTGGACCAGAGCTGGA
  AATATGCGGCTACGGATGTTGGGATCATTATTACGAGTCGGACACCCTCTTGCACTCGTTTCAAGTCCTA
  GCGGCCCTTCTGGAGTCTCCCGTCACTCAGGACATCATCTGCGACGTGGGGATGCCTGTAATGCACCGAA
  ACGTCCGCTACAACTGCAGAGTGATATTCCTCAACAGGAAGATCCTGCTCATCAGACCCAAGATGGCCTT
  GGCCAATGAAGGCAACTACCGCGAGCTGCGCTGGTTCACCCCGTGGTCGAGGAGTCGGCACACAGAGGAG
  TACTTTCTGCCTCGGATGATACAGGACCTGACAAAGCAGGAAACCGTACCCTTCGGAGATGCGGTGCTGG
  TGACATGGGACACCTGCATTGGAAGTGAGATCTGTGAGGAGCTCTGGACACCCCACAGCCCGCACATCGA
  CATGGGCCTGGATGGCGTGGAGATCATCACCAACGCCTCGGGCAGCCACCACGTGCTGCGCAAAGCCAAC
  ACCAGGGTGGATCTCGTGACTATGGTCACCAGCAAGAACGGTGGGATTTACTTGCTGGCCAACCAGAAGG
  GTTGCGACGGGGACCGCCTGTACTACGACGGCTGTGCCATGATCGCCATGAACGGAAGCGTCTTTGCTCA
  AGGATCCCAGTTTTCTCTGGATGACGTGGAAGTCCTGACGGCCACGCTGGATCTGGAGGACGTCCGGAGC
  TACAGGGCGGAGATTTCATCTCGAAACCTGGCGGCCAGCAGGGCGAGCCCCTACCCCAGAGTGAAGGTGG
  ACTTTGCCCTCTCGTGCCACGAGGACTTGCTGGCACCCATCTCTGAGCCCATCGAGTGGAAATACCACAG
  CCCTGAGGAGGAGATAAGCCTTGGACCTGCCTGCTGGCTCTGGGATTTTTTAAGACGAAGTCAACAGGCA
  GGGTTTTTGCTGCCCTTGAGTGGCGGGGTGGACAGCGCAGCCACCGCCTGCCTCATCTACTCCATGTGCT
  GCCAGGTCTGCGAGGCCGTGAGGAGTGGAAATGAGGAAGTGCTGGCTGATGTCCGCACCATCGTGAACCA
  GATCAGCTACACCCCCCAGGATCCCCGAGACCTCTGTGGACGCATACTGACCACCTGCTACATGGCCAGC
  AAGAACTCCTCCCAGGAGACGTGCACCCGGGCCAGAGAGTTGGCCCAGCAGATTGGAAGCCACCACATCA
  GTCTCAACATCGATCCAGCCGTGAAGGCCGTCATGGGCATCTTCAGCCTGGTGACGGGGAAGAGCCCTCT
  GTTTGCAGCTCATGGAGGAAGCAGCAGGGAAAACCTGGCGCTGCAAAATGTGCAGGCTCGAATACGGATG
  GTCCTCGCCTATCTGTTTGCTCAGTTGAGCCTCTGGTCTCGGGGTGTCCACGGTGGGCTCCTCGTGCTGG
  GATCCGCCAACGTGGATGAGAGTCTCCTGGGCTACCTGACCAAGTACGACTGCTCCAGTGCGGACATCAA
  CCCCATAGGCGGGATCAGCAAGACGGACCTCAGGGCCTTCGTCCAGTTCTGCATCCAGCGCTTCCAGCTT
  CCTGCCCTGCAGAGCATCCTGTTGGCGCCGGCCACCGCAGAGCTGGAGCCCTTGGCTGATGGACAGGTGT
  CCCAGACCGACGAGGAAGATATGGGGATGACATATGCGGAGCTCTCGGTCTATGGGAAACTCAGGAAGGT
  GGCCAAGATGGGGCCCTACAGCATGTTCTGCAAACTCCTCGGCATGTGGAGACACATCTGCACCCCGAGA
  CAGGTCGCTGACAAAGTGAAGCGGTTTTTCTCCAAGTACTCCATGAACAGACACAAGATGACCACGCTCA
  CACCCGCGTACCACGCCGAGAACTACAGCCCTGAGGACAACAGGTTTGATCTGCGACCATTTCTGTACAA
  CACAAGCTGGCCTTGGCAGTTTCGGTGCATAGAAAATCAGGTGCTACAGCTCGAGAGGGCAGAGCCACAG
  TCCCTGGACGGCGTGGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200131 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGRKVTVATCALNQWALDFEGNLQRILKSIEIAKNRGARYRLGPELEICGYGCWDHYYESDTLLHSFQVL
  AALLESPVTQDIICDVGMPVMHRNVRYNCRVIFLNRKILLIRPKMALANEGNYRELRWFTPWSRSRHTEE
  YFLPRMIQDLTKQETVPFGDAVLVTWDTCIGSEICEELWTPHSPHIDMGLDGVEIITNASGSHHVLRKAN
  TRVDLVTMVTSKNGGIYLLANQKGCDGDRLYYDGCAMIAMNGSVFAQGSQFSLDDVEVLTATLDLEDVRS
  YRAEISSRNLAASRASPYPRVKVDFALSCHEDLLAPISEPIEWKYHSPEEEISLGPACWLWDFLRRSQQA
  GFLLPLSGGVDSAATACLIYSMCCQVCEAVRSGNEEVLADVRTIVNQISYTPQDPRDLCGRILTTCYMAS
  KNSSQETCTRARELAQQIGSHHISLNIDPAVKAVMGIFSLVTGKSPLFAAHGGSSRENLALQNVQARIRM
  VLAYLFAQLSLWSRGVHGGLLVLGSANVDESLLGYLTKYDCSSADINPIGGISKTDLRAFVQFCIQRFQL
  PALQSILLAPATAELEPLADGQVSQTDEEDMGMTYAELSVYGKLRKVAKMGPYSMFCKLLGMWRHICTPR
  QVADKVKRFFSKYSMNRHKMTTLTPAYHAENYSPEDNRFDLRPFLYNTSWPWQFRCIENQVLQLERAEPQ
  SLDGVD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018161
• ORF Size: 2118 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018161.1, NM_018161.2, NM_018161.3, NM_018161.4, NP_060631.2
• RefSeq Size: 2453 bp
• RefSeq ORF: 2121 bp
• Locus ID: 55191
• Domains: CN_hydrolase, NAD_synthase
• Protein Pathways: Metabolic pathways, Nicotinate and nicotinamide metabolism
• MW: 79.3 kDa
• Gene Summary: Nicotinamide adenine dinucleotide (NAD) is a coenzyme in metabolic redox reactions, a precursor for several cell signaling molecules, and a substrate for protein posttranslational modifications. NAD synthetase (EC 6.3.5.1) catalyzes the final step in the biosynthesis of NAD from nicotinic acid adenine dinucleotide (NaAD). [supplied by OMIM, Apr 2004]