NDC1 (NM_018087) Human Tagged ORF Clone

CAT#: RC200141

TMEM48 (Myc-DDK-tagged)-Human transmembrane protein 48 (TMEM48), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY413329) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200141 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NDC1
• Synonyms: NET3; TMEM48
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200141 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCACGGCCGTGAGCCGGCCCTGCGCCGGCAGGTCGCGGGACATACTGTGGCGCGTTTTGGGCTGGA
  GGATAGTTGCAAGTATTGTTTGGTCAGTGCTATTTCTACCCATCTGCACCACAGTATTTATAATTTTCAG
  CAGGATTGATTTGTTTCATCCTATACAGTGGCTGTCTGATTCTTTCAGTGACCTGTATAGTTCCTATGTA
  ATCTTTTACTTCCTGCTGCTGTCAGTGGTAATAATAATAATAAGTATTTTCAATGTGGAGTTCTATGCAG
  TTGTGCCTTCTATTCCTTGCTCCAGACTAGCTCTGATAGGGAAGATCATTCATCCTCAGCAACTCATGCA
  CTCATTTATTCATGCTGCAATGGGAATGGTGATGGCCTGGTGTGCTGCAGTGATAACCCAGGGCCAGTAC
  AGCTTTCTTGTGGTTCCCTGCACTGGTACTAACAGCTTTGATAGCCCTGCTGCGCAAACCTGCTTAAATG
  AATATCATCTTTTTTTCCTACTGACTGGAGCATTTATGGGCTATAGCTATAGCCTCCTGTATTTTGTTAA
  CAACATGAACTATCTTCCATTTCCCATCATACAGCAATACAAGTTCTTGCGTTTTAGGAGATCTCTGCTC
  TTATTAGTTAAACACAGTTGTGTGGAATCACTGTTCCTGGTTAGAAATTTCTGCATTTTATATTATTTTC
  TTGGCTATATTCCCAAAGCTTGGATTAGCACTGCTATGAACCTTCACATAGATGAGCAGGTTCATAGGCC
  ACTTGACACAGTGAGTGGCCTCTTAAATCTCTCGTTACTCTACCATGTCTGGCTGTGTGGTGTCTTTCTC
  CTGACGACTTGGTATGTCTCATGGATACTCTTCAAAATCTATGCCACAGAGGCTCATGTGTTTCCTGTTC
  AACCACCATTTGCAGAAGGGTCAGATGAGTGCCTTCCAAAAGTGTTAAATAGCAATCCTCCCCCCATCAT
  AAAGTATTTAGCCTTGCAGGACCTGATGTTGCTTTCTCAATATTCTCCTTCACGAAGACAAGAAGTTTTC
  AGCCTCAGCCAACCAGGTGGACATCCCCACAATTGGACAGCCATTTCAAGGGAGTGTTTGAATCTTTTAA
  ATGGTATGACTCAGAAACTGATTCTCTATCAAGAAGCTGCTGCTACGAATGGGAGAGTGTCTTCATCTTA
  CCCAGTGGAACCTAAGAAATTAAATTCTCCAGAAGAAACTGCTTTTCAGACACCAAAATCTAGCCAGATG
  CCTCGGCCTTCAGTGCCACCATTAGTTAAAACATCACTGTTTTCTTCAAAATTATCTACACCTGATGTTG
  TGAGCCCATTTGGGACCCCATTTGGCTCTAGTGTAATGAATCGGATGGCTGGAATTTTTGATGTAAACAC
  CTGCTATGGGTCACCGCAAAGTCCTCAGCTAATAAGAAGGGGGCCAAGATTGTGGACATCAGCTTCTGAT
  CAGCAAATGACTGAATTTTCTAATCCTTCTCCATCTACCTCTATTAGTGCTGAGGGTAAGACAATGAGAC
  AACCCAGTGTGATTTATTCATGGATTCAGAATAAACGTGAACAGATTAAGAATTTCTTGTCAAAACGGGT
  GCTGATAATGTATTTTTTCAGTAAGCACCCAGAGGCCTCCATTCAGGCTGTTTTTTCAGATGCCCAAATG
  CATATTTGGGCATTAGAAGGTCTGTCGCACTTAGTAGCAGCATCATTTACAGAGGATAGATTTGGAGTTG
  TCCAGACGACACTACCAGCTATCCTTAATACTTTGTTGACACTGCAAGAGGCAGTCGACAAGTACTTTAA
  GCTTCCTCATGCTTCCAGTAAACCACCCCGGATTTCAGGAAGCCTTGTGGACACTTCATATAAAACATTA
  AGATTTGCATTCAGAGCATCACTGAAAACTGCCATCTATCGAATAACTACTACATTTGGTGAACATCTGA
  ATGCTGTGCAAGCATCTGCAGAACATCAGAAAAGACTTCAACAGTTCTTGGAGTTCAAAGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200141 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MATAVSRPCAGRSRDILWRVLGWRIVASIVWSVLFLPICTTVFIIFSRIDLFHPIQWLSDSFSDLYSSYV
  IFYFLLLSVVIIIISIFNVEFYAVVPSIPCSRLALIGKIIHPQQLMHSFIHAAMGMVMAWCAAVITQGQY
  SFLVVPCTGTNSFDSPAAQTCLNEYHLFFLLTGAFMGYSYSLLYFVNNMNYLPFPIIQQYKFLRFRRSLL
  LLVKHSCVESLFLVRNFCILYYFLGYIPKAWISTAMNLHIDEQVHRPLDTVSGLLNLSLLYHVWLCGVFL
  LTTWYVSWILFKIYATEAHVFPVQPPFAEGSDECLPKVLNSNPPPIIKYLALQDLMLLSQYSPSRRQEVF
  SLSQPGGHPHNWTAISRECLNLLNGMTQKLILYQEAAATNGRVSSSYPVEPKKLNSPEETAFQTPKSSQM
  PRPSVPPLVKTSLFSSKLSTPDVVSPFGTPFGSSVMNRMAGIFDVNTCYGSPQSPQLIRRGPRLWTSASD
  QQMTEFSNPSPSTSISAEGKTMRQPSVIYSWIQNKREQIKNFLSKRVLIMYFFSKHPEASIQAVFSDAQM
  HIWALEGLSHLVAASFTEDRFGVVQTTLPAILNTLLTLQEAVDKYFKLPHASSKPPRISGSLVDTSYKTL
  RFAFRASLKTAIYRITTTFGEHLNAVQASAEHQKRLQQFLEFKE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018087
• ORF Size: 2022 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018087.1, NM_018087.2, NM_018087.3, NM_018087.4, NP_060557.2
• RefSeq Size: 4840 bp
• RefSeq ORF: 2025 bp
• Locus ID: 55706
• Protein Families: Transmembrane
• MW: 76.4 kDa