DUSP26 (NM_024025) Human Tagged ORF Clone

CAT#: RC200202

DUSP26 (Myc-DDK-tagged)-Human dual specificity phosphatase 26 (putative) (DUSP26)

Product Images

Western blot validation of overexpression lysate (Cat# LY411403) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200202 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DUSP26
• Synonyms: DSP-4; DUSP24; LDP-4; LDP4; MKP-8; MKP8; NATA1; NEAP; SKRP3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200202 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGCCCTGGTAACTGGCTTTGGGCTTCTATGACTTTTATGGCCCGCTTCTCCCGGAGTAGCTCAAGGT
  CTCCTGTTCGAACTCGAGGGACCCTGGAGGAGATGCCAACCGTTCAACATCCTTTCCTCAATGTCTTCGA
  GTTGGAGCGGCTCCTCTACACAGGCAAGACAGCCTGTAACCATGCCGACGAGGTCTGGCCAGGCCTCTAT
  CTCGGAGACCAGGACATGGCTAACAACCGCCGGGAGCTTCGCCGCCTGGGCATCACGCACGTCCTCAATG
  CCTCACACAGCCGGTGGCGAGGCACGCCCGAGGCCTATGAGGGGCTGGGCATCCGCTACCTGGGTGTTGA
  GGCCCACGACTCGCCAGCCTTTGACATGAGCATCCACTTCCAGACGGCTGCCGACTTCATCCACCGGGCG
  CTGAGCCAGCCAGGAGGGAAGATCCTGGTGCATTGTGCTGTGGGCGTGAGCCGATCCGCCACCCTGGTAC
  TGGCCTACCTCATGCTGTACCACCACCTTACCCTCGTGGAGGCCATCAAGAAAGTCAAAGACCACCGAGG
  CATCATCCCCAACCGGGGCTTCCTGAGGCAGCTCCTGGCCCTGGACCGCAGGCTGCGGCAGGGTCTGGAA
  GCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200202 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MCPGNWLWASMTFMARFSRSSSRSPVRTRGTLEEMPTVQHPFLNVFELERLLYTGKTACNHADEVWPGLY
  LGDQDMANNRRELRRLGITHVLNASHSRWRGTPEAYEGLGIRYLGVEAHDSPAFDMSIHFQTAADFIHRA
  LSQPGGKILVHCAVGVSRSATLVLAYLMLYHHLTLVEAIKKVKDHRGIIPNRGFLRQLLALDRRLRQGLE
  A
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024025
• ORF Size: 633 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024025.1, NM_024025.2, NP_076930.1
• RefSeq Size: 1665 bp
• RefSeq ORF: 636 bp
• Locus ID: 78986
• Domains: DSPc
• Protein Families: Druggable Genome, Phosphatase
• MW: 23.9 kDa
• Gene Summary: This gene encodes a member of the tyrosine phosphatase family of proteins and exhibits dual specificity by dephosphorylating tyrosine as well as serine and threonine residues. This gene has been described as both a tumor suppressor and an oncogene depending on the cellular context. This protein may regulate neuronal proliferation and has been implicated in the progression of glioblastoma through its ability to dephosphorylate the p53 tumor suppressor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2015]