CNOT8 (NM_004779) Human Tagged ORF Clone
CAT#: RC200225
CNOT8 (Myc-DDK-tagged)-Human CCR4-NOT transcription complex, subunit 8 (CNOT8)
"NM_004779" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | CNOT8 |
Synonyms | CAF1; Caf1b; CALIF; hCAF1; POP2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC200225 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTGCAGCACTTGTGGAGAATAGCCAGGTTATCTGTGAAGTGTGGGCCAGTAATCTAGAAGAAGAGA TGAGGAAGATCCGAGAAATCGTGCTCAGTTACAGTTATATTGCCATGGACACAGAATTTCCAGGTGTTGT GGTGCGACCAATTGGTGAATTTCGTAGTTCCATAGATTACCAATATCAGCTTCTGCGGTGCAATGTTGAC CTTTTAAAAATTATCCAGCTGGGCCTTACATTCACAAATGAGAAGGGAGAGTATCCTTCTGGAATCAATA CTTGGCAGTTCAATTTCAAATTTAACCTTACAGAGGACATGTACTCCCAGGATTCCATAGATCTCCTTGC TAACTCAGGACTACAGTTTCAGAAGCATGAAGAGGAAGGGATTGACACACTGCACTTTGCAGAGCTGCTT ATGACATCAGGAGTGGTTCTCTGTGACAATGTCAAATGGCTTTCATTTCATAGTGGCTATGATTTTGGCT ATATGGTAAAGTTGCTTACAGATTCTCGTTTGCCAGAAGAGGAACATGAATTCTTTCATATTCTGAACCT TTTCTTCCCATCCATTTATGATGTGAAATACCTGATGAAGAGCTGCAAAAATCTTAAGGGAGGTCTTCAG GAAGTTGCTGATCAGTTGGATTTGCAGAGGATTGGAAGGCAGCACCAGGCAGGCTCAGACTCACTGCTGA CAGGAATGGCTTTCTTTAGGATGAAAGAGTTGTTTTTTGAGGACAGCATTGATGATGCCAAGTACTGTGG GCGGCTCTATGGCTTAGGCACAGGAGTGGCCCAGAAGCAGAATGAGGATGTGGACTCTGCCCAGGAGAAG ATGAGCATCCTGGCGATTATCAACAACATGCAGCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200225 protein sequence
Red=Cloning site Green=Tags(s) MPAALVENSQVICEVWASNLEEEMRKIREIVLSYSYIAMDTEFPGVVVRPIGEFRSSIDYQYQLLRCNVD LLKIIQLGLTFTNEKGEYPSGINTWQFNFKFNLTEDMYSQDSIDLLANSGLQFQKHEEEGIDTLHFAELL MTSGVVLCDNVKWLSFHSGYDFGYMVKLLTDSRLPEEEHEFFHILNLFFPSIYDVKYLMKSCKNLKGGLQ EVADQLDLQRIGRQHQAGSDSLLTGMAFFRMKELFFEDSIDDAKYCGRLYGLGTGVAQKQNEDVDSAQEK MSILAIINNMQQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_004779 |
ORF Size | 876 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_004779.1, NM_004779.2, NM_004779.3, NM_004779.4, NM_004779.5, NP_004770.4 |
RefSeq Size | 2620 bp |
RefSeq ORF | 879 bp |
Locus ID | 9337 |
Domains | CAF1 |
Protein Families | Transcription Factors |
Protein Pathways | RNA degradation |
MW | 33.5 kDa |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC117172 | CNOT8 (untagged)-Human CCR4-NOT transcription complex, subunit 8 (CNOT8) |
USD 310.00 |
|
RG200225 | CNOT8 (GFP-tagged) - Human CCR4-NOT transcription complex, subunit 8 (CNOT8) |
USD 460.00 |
|
RC200225L3 | Lenti ORF clone of Human CCR4-NOT transcription complex, subunit 8 (CNOT8), Myc-DDK-tagged |
USD 768.00 |
|
RC200225L4 | Lenti ORF clone of Human CCR4-NOT transcription complex, subunit 8 (CNOT8), mGFP tagged |
USD 768.00 |
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