TMED1 (NM_006858) Human Tagged ORF Clone

CAT#: RC200255

TMED1 (Myc-DDK-tagged)-Human transmembrane emp24 protein transport domain containing 1 (TMED1)

Product Images

Western blot validation of overexpression lysate (Cat# LY402049) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200255 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TMED1
• Synonyms: Il1rl1l; IL1RL1LG; p24g1; Tp24
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200255 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGGCGGCCGGCGCGGCCCTAGCCCTGGCCTTGTGGCTACTAATGCCACCAGTGGAGGTGGGAGGGG
  CGGGGCCCCCGCCAATCCAGGACGGTGAGTTCACGTTCCTGTTGCCGGCGGGGAGGAAGCAGTGTTTCTA
  CCAGTCCGCGCCGGCCAACGCAAGCCTCGAGACCGAATACCAGGTGATCGGAGGTGCTGGACTGGACGTG
  GACTTCACGCTGGAGAGCCCTCAGGGCGTGCTGTTGGTCAGCGAGTCCCGCAAGGCTGATGGGGTACACA
  CGGTGGAGCCAACGGAGGCCGGGGACTACAAGCTGTGCTTTGACAACTCCTTCAGCACCATCTCCGAGAA
  GCTGGTGTTCTTTGAACTGATCTTTGACAGCCTCCAGGATGACGAGGAGGTCGAAGGATGGGCAGAGGCT
  GTGGAGCCCGAGGAGATGCTGGATGTTAAAATGGAGGACATCAAGGAGTCCATTGAGACCATGCGGACCC
  GGCTGGAGCGCAGCATCCAGATGCTCACGCTACTGCGGGCCTTCGAGGCACGTGACCGCAACCTGCAAGA
  GGGCAACTTGGAGCGGGTCAACTTCTGGTCAGCTGTCAACGTGGCGGTGCTGCTGCTGGTGGCTGTGCTG
  CAGGTCTGCACGCTCAAGCGCTTCTTCCAGGACAAGCGCCCGGTGCCCACG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200255 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MMAAGAALALALWLLMPPVEVGGAGPPPIQDGEFTFLLPAGRKQCFYQSAPANASLETEYQVIGGAGLDV
  DFTLESPQGVLLVSESRKADGVHTVEPTEAGDYKLCFDNSFSTISEKLVFFELIFDSLQDDEEVEGWAEA
  VEPEEMLDVKMEDIKESIETMRTRLERSIQMLTLLRAFEARDRNLQEGNLERVNFWSAVNVAVLLLVAVL
  QVCTLKRFFQDKRPVPT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006858
• ORF Size: 681 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006858.1, NM_006858.2, NM_006858.3, NP_006849.1
• RefSeq Size: 1739 bp
• RefSeq ORF: 684 bp
• Locus ID: 11018
• Protein Families: Druggable Genome, Transmembrane
• MW: 25.2 kDa
• Gene Summary: This gene belongs to the TMED (transmembrane emp24 domain-containing) protein family, which is involved in the vesicular trafficking of proteins. The protein encoded by this gene was identified by its interaction with interleukin 1 receptor-like 1 (IL1RL1) and may play a role in innate immunity. This protein lacks any similarity to other interleukin 1 ligands. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]