MTHFD1 (NM_005956) Human Tagged ORF Clone

CAT#: RC200297

MTHFD1 (Myc-DDK-tagged)-Human methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase (MTHFD1)

Product Images

Western blot validation of overexpression lysate (Cat# LY416959) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200297 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified MTHFD1 protein (Cat# TP300297). The protein was produced from HEK293T cells transfected with MTHFD1 cDNA clone (Cat# RC200297) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MTHFD1
• Synonyms: CIMAH; MTHFC; MTHFD
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200297 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCCAGCAGAAATCCTGAACGGGAAGGAGATCTCCGCGCAAATAAGGGCGAGACTGAAAAATCAAG
  TCACTCAGTTGAAGGAGCAAGTACCTGGTTTCACACCACGCCTGGCAATATTACAGGTTGGCAACAGAGA
  TGATTCCAATCTTTATATAAATGTGAAGCTGAAGGCTGCTGAAGAGATTGGGATCAAAGCCACTCACATT
  AAGTTACCAAGAACAACCACAGAATCTGAGGTGATGAAGTACATTACATCTTTGAATGAAGACTCTACTG
  TACATGGGTTCTTAGTGCAGCTACCTTTAGATTCAGAGAATTCCATTAACACTGAAGAAGTGATCAATGC
  TATTGCACCCGAGAAGGATGTGGATGGATTGACTAGCATCAATGCTGGGAGACTTGCTAGAGGTGACCTC
  AATGACTGTTTCATTCCTTGTACGCCTAAGGGATGCTTGGAACTCATCAAAGAGACAGGGGTGCCGATTG
  CCGGAAGGCATGCTGTGGTGGTTGGGCGCAGTAAAATAGTTGGGGCCCCGATGCATGACTTGCTTCTGTG
  GAACAATGCCACAGTGACCACCTGCCACTCCAAGACTGCCCATCTGGATGAGGAGGTAAATAAAGGTGAC
  ATCCTGGTGGTTGCAACTGGTCAGCCTGAAATGGTTAAAGGGGAGTGGATCAAACCTGGGGCAATAGTCA
  TCGACTGTGGAATCAATTATGTCCCAGATGATAAAAAACCAAATGGGAGAAAAGTTGTGGGTGATGTGGC
  ATACGACGAGGCCAAAGAGAGGGCGAGCTTCATCACTCCTGTTCCTGGCGGCGTAGGGCCCATGACAGTT
  GCAATGCTCATGCAGAGCACAGTAGAGAGTGCCAAGCGTTTCCTGGAGAAATTTAAGCCAGGAAAGTGGA
  TGATTCAGTATAACAACCTTAACCTCAAGACACCTGTTCCAAGTGACATTGATATATCACGATCTTGTAA
  ACCGAAGCCCATTGGTAAGCTGGCTCGAGAAATTGGTCTGCTGTCTGAAGAGGTAGAATTATATGGTGAA
  ACAAAGGCCAAAGTTCTGCTGTCAGCACTAGAACGCCTGAAGCACCGGCCTGATGGGAAATACGTGGTGG
  TGACTGGAATAACTCCAACACCCCTGGGAGAAGGGAAAAGCACAACTACAATCGGGCTAGTGCAAGCCCT
  TGGTGCCCATCTCTACCAGAATGTCTTTGCGTGTGTGCGACAGCCTTCTCAGGGCCCCACCTTTGGAATA
  AAAGGTGGCGCTGCAGGAGGCGGCTACTCCCAGGTCATTCCTATGGAAGAGTTTAATCTCCACCTCACAG
  GTGACATCCATGCCATCACTGCAGCTAATAACCTCGTTGCTGCGGCCATTGATGCTCGGATATTTCATGA
  ACTGACCCAGACAGACAAGGCTCTCTTTAATCGTTTGGTGCCATCAGTAAATGGAGTGAGAAGGTTCTCT
  GACATCCAAATCCGAAGGTTAAAGAGACTAGGCATTGAAAAGACTGACCCTACCACACTGACAGATGAAG
  AGATAAACAGATTTGCAAGATTGGACATTGATCCAGAAACCATAACTTGGCAAAGAGTGTTGGATACCAA
  TGATAGATTCCTGAGGAAGATCACGATTGGACAGGCTCCAACGGAGAAGGGTCACACACGGACGGCCCAG
  TTTGATATCTCTGTGGCCAGTGAAATTATGGCTGTCCTGGCTCTCACCACTTCTCTAGAAGACATGAGAG
  AGAGACTGGGCAAAATGGTGGTGGCATCCAGTAAGAAAGGAGAGCCCGTCAGTGCCGAAGATCTGGGGGT
  GAGTGGTGCACTGACAGTGCTTATGAAGGACGCAATCAAGCCCAATCTCATGCAGACACTGGAGGGCACT
  CCAGTGTTTGTCCATGCTGGCCCGTTTGCCAACATCGCACATGGCAATTCCTCCATCATTGCAGACCAGA
  TCGCACTCAAGCTTGTTGGCCCAGAAGGGTTTGTAGTGACGGAAGCAGGATTTGGAGCAGACATTGGAAT
  GGAAAAGTTTTTTAACATCAAATGCCGGTATTCCGGCCTCTGCCCCCACGTGGTGGTGCTTGTTGCCACT
  GTCAGGGCTCTCAAGATGCACGGGGGCGGCCCCACGGTCACTGCTGGACTGCCTCTTCCCAAGGCTTACA
  TACAGGAGAACCTGGAGCTGGTTGAAAAAGGCTTCAGTAACTTGAAGAAACAAATTGAAAATGCCAGAAT
  GTTTGGAATTCCAGTAGTAGTGGCCGTGAATGCATTCAAGACGGATACAGAGTCTGAGCTGGACCTCATC
  AGCCGCCTTTCCAGAGAACATGGGGCTTTTGATGCCGTGAAGTGCACTCACTGGGCAGAAGGGGGCAAGG
  GTGCCTTAGCCCTGGCTCAGGCCGTCCAGAGAGCAGCACAAGCACCCAGCAGCTTCCAGCTCCTTTATGA
  CCTCAAGCTCCCAGTTGAGGATAAAATCAGGATCATTGCACAGAAGATCTATGGAGCAGATGACATTGAA
  TTACTTCCCGAAGCTCAACACAAAGCTGAAGTCTACACGAAGCAGGGCTTTGGGAATCTCCCCATCTGCA
  TGGCTAAAACACACTTGTCTTTGTCTCACAACCCAGAGCAAAAAGGTGTCCCTACAGGCTTCATTCTGCC
  CATTCGCGACATCCGCGCCAGCGTTGGGGCTGGTTTTCTGTACCCCTTAGTAGGAACGATGAGCACAATG
  CCTGGACTCCCCACCCGGCCCTGTTTTTATGATATTGATTTGGACCCTGAAACAGAACAGGTGAATGGAT
  TATTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200297 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAPAEILNGKEISAQIRARLKNQVTQLKEQVPGFTPRLAILQVGNRDDSNLYINVKLKAAEEIGIKATHI
  KLPRTTTESEVMKYITSLNEDSTVHGFLVQLPLDSENSINTEEVINAIAPEKDVDGLTSINAGRLARGDL
  NDCFIPCTPKGCLELIKETGVPIAGRHAVVVGRSKIVGAPMHDLLLWNNATVTTCHSKTAHLDEEVNKGD
  ILVVATGQPEMVKGEWIKPGAIVIDCGINYVPDDKKPNGRKVVGDVAYDEAKERASFITPVPGGVGPMTV
  AMLMQSTVESAKRFLEKFKPGKWMIQYNNLNLKTPVPSDIDISRSCKPKPIGKLAREIGLLSEEVELYGE
  TKAKVLLSALERLKHRPDGKYVVVTGITPTPLGEGKSTTTIGLVQALGAHLYQNVFACVRQPSQGPTFGI
  KGGAAGGGYSQVIPMEEFNLHLTGDIHAITAANNLVAAAIDARIFHELTQTDKALFNRLVPSVNGVRRFS
  DIQIRRLKRLGIEKTDPTTLTDEEINRFARLDIDPETITWQRVLDTNDRFLRKITIGQAPTEKGHTRTAQ
  FDISVASEIMAVLALTTSLEDMRERLGKMVVASSKKGEPVSAEDLGVSGALTVLMKDAIKPNLMQTLEGT
  PVFVHAGPFANIAHGNSSIIADQIALKLVGPEGFVVTEAGFGADIGMEKFFNIKCRYSGLCPHVVVLVAT
  VRALKMHGGGPTVTAGLPLPKAYIQENLELVEKGFSNLKKQIENARMFGIPVVVAVNAFKTDTESELDLI
  SRLSREHGAFDAVKCTHWAEGGKGALALAQAVQRAAQAPSSFQLLYDLKLPVEDKIRIIAQKIYGADDIE
  LLPEAQHKAEVYTKQGFGNLPICMAKTHLSLSHNPEQKGVPTGFILPIRDIRASVGAGFLYPLVGTMSTM
  PGLPTRPCFYDIDLDPETEQVNGLF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005956
• ORF Size: 2805 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005956.1, NM_005956.2, NM_005956.3, NP_005947.2
• RefSeq Size: 3466 bp
• RefSeq ORF: 2808 bp
• Locus ID: 4522
• Cytogenetics: 14q23.3
• Domains: FTHFS, THF_DHG_CYH
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Glyoxylate and dicarboxylate metabolism, Metabolic pathways, One carbon pool by folate
• MW: 101.5 kDa
• Gene Summary: 'This gene encodes a protein that possesses three distinct enzymatic activities, 5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methenyltetrahydrofolate cyclohydrolase and 10-formyltetrahydrofolate synthetase. Each of these activities catalyzes one of three sequential reactions in the interconversion of 1-carbon derivatives of tetrahydrofolate, which are substrates for methionine, thymidylate, and de novo purine syntheses. The trifunctional enzymatic activities are conferred by two major domains, an aminoterminal portion containing the dehydrogenase and cyclohydrolase activities and a larger synthetase domain. [provided by RefSeq, Jul 2008]'