Angiopoietin like 7 (ANGPTL7) (NM_021146) Human Tagged ORF Clone

CAT#: RC200321

ANGPTL7 (Myc-DDK-tagged)-Human angiopoietin-like 7 (ANGPTL7)

Product Images

Western blot validation of overexpression lysate (Cat# LY402846) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200321 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ANGPTL7 protein (Cat# TP300321). The protein was produced from HEK293T cells transfected with ANGPTL7 cDNA clone (Cat# RC200321) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ANGPTL7
• Synonyms: AngX; CDT6; dJ647M16.1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200321 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGAAAAAGCCTCTCTCAGCTGTGACCTGGCTCTGCATTTTCATCGTGGCCTTTGTCAGCCACCCAG
  CGTGGCTGCAGAAGCTCTCTAAGCACAAGACACCAGCACAGCCACAGCTCAAAGCGGCCAACTGCTGTGA
  GGAGGTGAAGGAGCTCAAGGCCCAAGTTGCCAACCTTAGCAGCCTGCTGAGTGAACTGAACAAGAAGCAG
  GAGAGGGACTGGGTCAGCGTGGTCATGCAGGTGATGGAGCTGGAGAGCAACAGCAAGCGCATGGAGTCGC
  GGCTCACAGATGCTGAGAGCAAGTACTCCGAGATGAACAACCAAATTGACATCATGCAGCTGCAGGCAGC
  ACAGACGGTCACTCAGACCTCCGCAGATGCCATCTACGACTGCTCTTCCCTCTACCAGAAGAACTACCGC
  ATCTCTGGAGTGTATAAGCTTCCTCCTGATGACTTCCTGGGCAGCCCTGAACTGGAGGTGTTCTGTGACA
  TGGAGACTTCAGGCGGAGGCTGGACCATCATCCAGAGACGAAAAAGTGGCCTTGTCTCCTTCTACCGGGA
  CTGGAAGCAGTACAAGCAGGGCTTTGGCAGCATCCGTGGGGACTTCTGGCTGGGGAACGAACACATCCAC
  CGGCTCTCCAGACAGCCAACCCGGCTGCGTGTAGAGATGGAGGACTGGGAGGGCAACCTGCGCTACGCTG
  AGTATAGCCACTTTGTTTTGGGCAATGAACTCAACAGCTATCGCCTCTTCCTGGGGAACTACACTGGCAA
  TGTGGGGAACGACGCCCTCCAGTATCATAACAACACAGCCTTCAGCACCAAGGACAAGGACAATGACAAC
  TGCTTGGACAAGTGTGCACAGCTCCGCAAAGGTGGCTACTGGTACAACTGCTGCACAGACTCCAACCTCA
  ATGGAGTGTACTACCGCCTGGGTGAGCACAATAAGCACCTGGATGGCATCACCTGGTATGGCTGGCATGG
  ATCTACCTACTCCCTCAAACGGGTGGAGATGAAAATCCGCCCAGAAGACTTCAAGCCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200321 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLKKPLSAVTWLCIFIVAFVSHPAWLQKLSKHKTPAQPQLKAANCCEEVKELKAQVANLSSLLSELNKKQ
  ERDWVSVVMQVMELESNSKRMESRLTDAESKYSEMNNQIDIMQLQAAQTVTQTSADAIYDCSSLYQKNYR
  ISGVYKLPPDDFLGSPELEVFCDMETSGGGWTIIQRRKSGLVSFYRDWKQYKQGFGSIRGDFWLGNEHIH
  RLSRQPTRLRVEMEDWEGNLRYAEYSHFVLGNELNSYRLFLGNYTGNVGNDALQYHNNTAFSTKDKDNDN
  CLDKCAQLRKGGYWYNCCTDSNLNGVYYRLGEHNKHLDGITWYGWHGSTYSLKRVEMKIRPEDFKP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021146
• ORF Size: 1038 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021146.1, NM_021146.2, NM_021146.3, NP_066969.1
• RefSeq Size: 2307 bp
• RefSeq ORF: 1041 bp
• Locus ID: 10218
• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• MW: 40 kDa