XRCC3 (NM_005432) Human Tagged ORF Clone
CAT#: RC200326
XRCC3 (Myc-DDK-tagged)-Human X-ray repair complementing defective repair in Chinese hamster cells 3 (XRCC3), transcript variant 2
"NM_005432" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | XRCC3 |
Synonyms | CMM6 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC200326 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATTTGGATCTACTGGACCTGAATCCCAGAATTATTGCTGCAATTAAGAAAGCCAAACTGAAATCGG TAAAGGAGGTTTTACACTTTTCTGGACCAGACTTGAAGAGACTGACCAACCTCTCCAGCCCCGAGGTCTG GCACTTGCTGAGAACGGCCTCCTTACACTTGCGGGGAAGCAGCATCCTTACAGCACTGCAGCTGCACCAG CAGAAGGAGCGGTTCCCCACGCAGCACCAGCGCCTGAGCCTGGGCTGCCCGGTGCTGGACGCGCTGCTCC GCGGTGGCCTGCCCCTGGACGGCATCACTGAGCTGGCCGGACGCAGCTCGGCAGGGAAGACCCAGCTGGC GCTGCAGCTCTGCCTGGCTGTGCAGTTCCCGCGGCAGCACGGAGGCCTGGAGGCTGGAGCCGTCTACATC TGCACGGAAGACGCCTTCCCGCACAAGCGCCTGCAGCAGCTCATGGCCCAGCAGCCGCGGCTGCGCACTG ACGTTCCAGGAGAGCTGCTTCAGAAGCTCCGATTTGGCAGCCAGATCTTCATCGAGCACGTGGCCGATGT GGACACCTTGTTGGAGTGTGTGAATAAGAAGGTCCCCGTACTGCTGTCTCGGGGCATGGCTCGCCTGGTG GTCATCGACTCGGTGGCAGCCCCATTCCGCTGTGAATTTGACAGCCAGGCCTCCGCCCCCAGGGCCAGGC ATCTGCAGTCCCTGGGGGCCACGCTGCGTGAGCTGAGCAGTGCCTTCCAGAGCCCTGTGCTGTGCATCAA CCAGGTGACAGAGGCCATGGAGGAGCAGGGCGCAGCACACGGGCCGCTGGGGTTCTGGGACGAACGTGTT TCCCCAGCCCTTGGCATAACCTGGGCTAACCAGCTCCTGGTGAGACTGCTGGCTGACCGGCTCCGCGAGG AAGAGGCTGCCCTCGGCTGCCCAGCCCGGACCCTGCGGGTGCTCTCTGCCCCCCACCTGCCCCCCTCCTC CTGTTCCTACACGATCAGTGCCGAAGGGGTGCGAGGGACACCTGGGACCCAGTCCCAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200326 protein sequence
Red=Cloning site Green=Tags(s) MDLDLLDLNPRIIAAIKKAKLKSVKEVLHFSGPDLKRLTNLSSPEVWHLLRTASLHLRGSSILTALQLHQ QKERFPTQHQRLSLGCPVLDALLRGGLPLDGITELAGRSSAGKTQLALQLCLAVQFPRQHGGLEAGAVYI CTEDAFPHKRLQQLMAQQPRLRTDVPGELLQKLRFGSQIFIEHVADVDTLLECVNKKVPVLLSRGMARLV VIDSVAAPFRCEFDSQASAPRARHLQSLGATLRELSSAFQSPVLCINQVTEAMEEQGAAHGPLGFWDERV SPALGITWANQLLVRLLADRLREEEAALGCPARTLRVLSAPHLPPSSCSYTISAEGVRGTPGTQSH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_005432 |
ORF Size | 1038 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_005432.1, NM_005432.2, NM_005432.3, NP_005423.1 |
RefSeq Size | 2620 bp |
RefSeq ORF | 1041 bp |
Locus ID | 7517 |
Cytogenetics | 14q32.33 |
Protein Families | Druggable Genome |
Protein Pathways | Homologous recombination |
MW | 37.8 kDa |
Gene Summary | 'This gene encodes a member of the RecA/Rad51-related protein family that participates in homologous recombination to maintain chromosome stability and repair DNA damage. This gene functionally complements Chinese hamster irs1SF, a repair-deficient mutant that exhibits hypersensitivity to a number of different DNA-damaging agents and is chromosomally unstable. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. Alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq, Jul 2008]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC116733 | XRCC3 (untagged)-Human X-ray repair complementing defective repair in Chinese hamster cells 3 (XRCC3), transcript variant 2 |
USD 310.00 |
|
RG200326 | XRCC3 (GFP-tagged) - Human X-ray repair complementing defective repair in Chinese hamster cells 3 (XRCC3), transcript variant 2 |
USD 460.00 |
|
RC200326L1 | Lenti ORF clone of Human X-ray repair complementing defective repair in Chinese hamster cells 3 (XRCC3), transcript variant 2, Myc-DDK-tagged |
USD 768.00 |
|
RC200326L2 | Lenti ORF clone of Human X-ray repair complementing defective repair in Chinese hamster cells 3 (XRCC3), transcript variant 2, mGFP tagged |
USD 768.00 |
|
RC200326L3 | Lenti ORF clone of Human X-ray repair complementing defective repair in Chinese hamster cells 3 (XRCC3), transcript variant 2, Myc-DDK-tagged |
USD 768.00 |
|
RC200326L4 | Lenti ORF clone of Human X-ray repair complementing defective repair in Chinese hamster cells 3 (XRCC3), transcript variant 2, mGFP tagged |
USD 768.00 |
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