VAMP8 (NM_003761) Human Tagged ORF Clone
CAT#: RC200405
VAMP8 (Myc-DDK-tagged)-Human vesicle-associated membrane protein 8 (endobrevin) (VAMP8)
"NM_003761" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | VAMP8 |
Synonyms | EDB; VAMP-8 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC200405 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGAAGCCAGTGAAGGTGGAGGAAATGATCGTGTGCGGAACCTGCAAAGTGAGGTGGAGGGAGTTA AGAATATTATGACCCAGAATGTGGAGCGGATCCTGGCCCGGGGGGAAAACTTGGAACATCTCCGCAACAA GACAGAGGATCTGGAAGCCACATCTGAGCACTTCAAGACGACATCGCAGAAGGTGGCTCGGAAATTCTGG TGGAAGAACGTGAAGATGATTGTCCTTATCTGCGTGATTGTTTTTATCATCATCCTCTTCATTGTGCTCT TTGCCACTGGTGCCTTCTCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200405 protein sequence
Red=Cloning site Green=Tags(s) MEEASEGGGNDRVRNLQSEVEGVKNIMTQNVERILARGENLEHLRNKTEDLEATSEHFKTTSQKVARKFW WKNVKMIVLICVIVFIIILFIVLFATGAFS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_003761 |
ORF Size | 300 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_003761.1, NM_003761.2, NM_003761.3, NM_003761.4, NP_003752.2 |
RefSeq Size | 790 bp |
RefSeq ORF | 303 bp |
Locus ID | 8673 |
Domains | synaptobrevin |
Protein Families | Stem cell - Pluripotency, Transmembrane |
Protein Pathways | SNARE interactions in vesicular transport |
MW | 11.4 kDa |
Gene Summary | This gene encodes an integral membrane protein that belongs to the synaptobrevin/vesicle-associated membrane protein subfamily of soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs). The encoded protein is involved in the fusion of synaptic vesicles with the presynaptic membrane. [provided by RefSeq, Jun 2010] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC117746 | VAMP8 (untagged)-Human vesicle-associated membrane protein 8 (endobrevin) (VAMP8) |
USD 310.00 |
|
RG200405 | VAMP8 (GFP-tagged) - Human vesicle-associated membrane protein 8 (endobrevin) (VAMP8) |
USD 460.00 |
|
RC200405L1 | Lenti ORF clone of Human vesicle-associated membrane protein 8 (endobrevin) (VAMP8), Myc-DDK-tagged |
USD 620.00 |
|
RC200405L2 | Lenti ORF clone of Human vesicle-associated membrane protein 8 (endobrevin) (VAMP8), mGFP tagged |
USD 620.00 |
|
RC200405L3 | Lenti ORF clone of Human vesicle-associated membrane protein 8 (endobrevin) (VAMP8), Myc-DDK-tagged |
USD 620.00 |
|
RC200405L4 | Lenti ORF clone of Human vesicle-associated membrane protein 8 (endobrevin) (VAMP8), mGFP tagged |
USD 620.00 |
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