PSMB10 (NM_002801) Human Tagged ORF Clone

CAT#: RC200437

PSMB10 (Myc-DDK-tagged)-Human proteasome (prosome, macropain) subunit, beta type, 10 (PSMB10)

Product Images

Western blot validation of overexpression lysate (Cat# LY419099) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200437 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PSMB10
• Synonyms: beta2i; LMP10; MECL1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200437 representing NM_002801
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGAAGCCAGCCCTGGAGCCCCGAGGGGGCTTCTCCTTCGAGAACTGCCAAAGAAATGCATCATTGG
  AACGCGTCCTCCCGGGGCTCAAGGTCCCTCACGCACGCAAGACCGGGACCACCATCGCGGGCCTGGTGTT
  CCAAGACGGGGTCATTCTGGGCGCCGATACGCGAGCCACTAACGATTCGGTCGTGGCGGACAAGAGCTGC
  GAGAAGATCCACTTCATCGCCCCCAAAATCTACTGCTGTGGGGCTGGAGTAGCCGCGGACGCCGAGATGA
  CCACACGGATGGTGGCGTCCAAGATGGAGCTACACGCGCTATCTACGGGCCGCGAGCCCCGCGTGGCCAC
  GGTCACTCGCATCCTGCGCCAGACGCTCTTCAGGTACCAGGGCCACGTGGGTGCATCGCTGATCGTGGGC
  GGCGTAGACCTGACTGGACCGCAGCTCTACGGTGTGCATCCCCATGGCTCCTACAGCCGTCTGCCCTTCA
  CAGCCCTGGGCTCTGGTCAGGACGCGGCCCTGGCGGTGCTAGAAGACCGGTTCCAGCCGAACATGACGCT
  GGAGGCTGCTCAGGGGCTGCTGGTGGAAGCCGTCACCGCCGGGATCTTGGGTGACCTGGGCTCCGGGGGC
  AATGTGGACGCATGTGTGATCACAAAGACTGGCGCCAAGCTGCTGCGGACACTGAGCTCACCCACAGAGC
  CCGTGAAGAGGTCTGGCCGCTACCACTTTGTGCCTGGAACCACAGCTGTCCTGACCCAGACAGTGAAGCC
  ACTAACCCTGGAGCTAGTGGAGGAAACTGTGCAGGCTATGGAGGTGGAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200437 representing NM_002801
  Red=Cloning site Green=Tags(s)
  
  MLKPALEPRGGFSFENCQRNASLERVLPGLKVPHARKTGTTIAGLVFQDGVILGADTRATNDSVVADKSC
  EKIHFIAPKIYCCGAGVAADAEMTTRMVASKMELHALSTGREPRVATVTRILRQTLFRYQGHVGASLIVG
  GVDLTGPQLYGVHPHGSYSRLPFTALGSGQDAALAVLEDRFQPNMTLEAAQGLLVEAVTAGILGDLGSGG
  NVDACVITKTGAKLLRTLSSPTEPVKRSGRYHFVPGTTAVLTQTVKPLTLELVEETVQAMEVE
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002801
• ORF Size: 819 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002801.1, NM_002801.2, NM_002801.3, NP_002792.1
• RefSeq Size: 1009 bp
• RefSeq ORF: 822 bp
• Locus ID: 5699
• Cytogenetics: 16q22.1
• Domains: proteasome
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Proteasome
• MW: 28.8 kDa
• Gene Summary: 'The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. Proteolytic processing is required to generate a mature subunit. Expression of this gene is induced by gamma interferon, and this gene product replaces catalytic subunit 2 (proteasome beta 7 subunit) in the immunoproteasome. [provided by RefSeq, Jul 2008]'