GALK2 (NM_002044) Human Tagged ORF Clone

CAT#: RC200475

GALK2 (Myc-DDK-tagged)-Human galactokinase 2 (GALK2), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY400749) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200475 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified GALK2 protein (Cat# TP300475). The protein was produced from HEK293T cells transfected with GALK2 cDNA clone (Cat# RC200475) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GALK2
• Synonyms: GK2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200475 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTACAGAGAGCCCTGCTACGCGTCGGGTCCAGGTGGCAGAACATCCTAGGTTACTGAAGCTAAAGG
  AGATGTTTAACTCCAAGTTTGGATCTATTCCCAAGTTTTATGTTCGAGCACCAGGAAGAGTCAACATAAT
  AGGAGAGCATATAGATTATTGTGGATATTCTGTTCTTCCTATGGCTGTAGAACAAGATGTGCTAATAGCT
  GTAGAACCTGTGAAAACGTACGCTCTCCAACTGGCCAATACAAATCCCTTGTATCCGGACTTCAGTACTA
  GTGCTAATAACATCCAGATTGATAAAACCAAGCCTTTGTGGCACAACTATTTCTTATGTGGACTTAAAGG
  AATTCAGGAACACTTTGGTCTTAGTAACCTGACTGGAATGAACTGCCTGGTAGATGGAAATATCCCACCA
  AGTTCTGGCCTCTCCAGCTCCAGTGCTTTGGTCTGTTGTGCTGGCTTGGTGACGCTCACAGTGCTGGGAA
  GGAATCTATCCAAGGTGGAACTTGCAGAAATCTGTGCCAAGAGTGAGCGTTACATTGGCACTGAAGGAGG
  AGGCATGGACCAGTCTATATCATTTCTTGCAGAAGAAGGAACTGCCAAGTTGATAGAATTTAGTCCTCTG
  AGGGCAACCGATGTAAAACTCCCAAGTGGAGCAGTGTTTGTGATTGCCAACAGTTGTGTGGAGATGAATA
  AGGCAGCAACTTCCCATTTCAATATCAGGGTGATGGAGTGTCGGCTGGCTGCGAAGCTCCTGGCTAAATA
  CAAAAGCTTGCAATGGGACAAAGTACTGAGGCTGGAGGAGGTGCAGGCTAAACTAGGGATTAGTCTAGAA
  GAAATGCTGTTGGTCACAGAAGATGCCCTTCATCCTGAACCCTATAACCCTGAGGAGATCTGCAGGTGTC
  TGGGAATTAGCCTGGAGGAACTCCGAACCCAAATCCTGAGTCCAAACACTCAAGATGTGCTCATCTTCAA
  ACTCTATCAGCGGGCAAAGCATGTGTACAGCGAGGCTGCGCGAGTGCTCCAGTTTAAGAAGATATGTGAA
  GAAGCACCTGAAAACATGGTCCAGCTGCTGGGAGAGTTGATGAACCAGAGCCACATGAGCTGCCGGGACA
  TGTATGAGTGCAGCTGCCCCGAGCTGGATCAGCTGGTGGACATCTGTCGGAAGTTTGGGGCTCAAGGGTC
  ACGACTTACTGGAGCAGGATGGGGAGGCTGTACAGTATCAATGGTACCTGCGGACAAGCTGCCCAGCTTT
  CTAGCAAATGTGCACAAAGCTTATTACCAGAGGAGTGATGGAAGCTTAGCACCGGAGAAGCAAAGTTTGT
  TTGCTACCAAACCTGGAGGTGGGGCTTTGGTTTTGCTTGAGGCC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200475 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MATESPATRRVQVAEHPRLLKLKEMFNSKFGSIPKFYVRAPGRVNIIGEHIDYCGYSVLPMAVEQDVLIA
  VEPVKTYALQLANTNPLYPDFSTSANNIQIDKTKPLWHNYFLCGLKGIQEHFGLSNLTGMNCLVDGNIPP
  SSGLSSSSALVCCAGLVTLTVLGRNLSKVELAEICAKSERYIGTEGGGMDQSISFLAEEGTAKLIEFSPL
  RATDVKLPSGAVFVIANSCVEMNKAATSHFNIRVMECRLAAKLLAKYKSLQWDKVLRLEEVQAKLGISLE
  EMLLVTEDALHPEPYNPEEICRCLGISLEELRTQILSPNTQDVLIFKLYQRAKHVYSEAARVLQFKKICE
  EAPENMVQLLGELMNQSHMSCRDMYECSCPELDQLVDICRKFGAQGSRLTGAGWGGCTVSMVPADKLPSF
  LANVHKAYYQRSDGSLAPEKQSLFATKPGGGALVLLEA
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002044
• ORF Size: 1374 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002044.1, NM_002044.2, NM_002044.3, NP_002035.1
• RefSeq Size: 3330 bp
• RefSeq ORF: 1377 bp
• Locus ID: 2585
• Cytogenetics: 15q21.1-q21.2
• Domains: GHMP_kinases
• Protein Families: Druggable Genome
• Protein Pathways: Amino sugar and nucleotide sugar metabolism, Galactose metabolism, Metabolic pathways
• MW: 50.4 kDa
• Gene Summary: 'This gene encodes a highly efficient N-acetylgalactosamine (GalNAc) kinase, which has galactokinase activity when galactose is present at high concentrations. The encoded protein is a member of the GHMP kinase family. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2017]'