VAPB (NM_004738) Human Tagged ORF Clone
CAT#: RC200517
VAPB (Myc-DDK-tagged)-Human VAMP (vesicle-associated membrane protein)-associated protein B and C (VAPB), transcript variant 1
"NM_004738" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | VAPB |
Synonyms | ALS8; VAMP-B; VAP-B |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC200517 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGAAGGTGGAGCAGGTCCTGAGCCTCGAGCCGCAGCACGAGCTCAAATTCCGAGGTCCCTTCACCG ATGTTGTCACCACCAACCTAAAGCTTGGCAACCCGACAGACCGAAATGTGTGTTTTAAGGTGAAGACTAC AGCACCACGTAGGTACTGTGTGAGGCCCAACAGCGGAATCATCGATGCAGGGGCCTCAATTAATGTATCT GTGATGTTACAGCCTTTCGATTATGATCCCAATGAGAAAAGTAAACACAAGTTTATGGTTCAGTCTATGT TTGCTCCAACTGACACTTCAGATATGGAAGCAGTATGGAAGGAGGCAAAACCGGAAGACCTTATGGATTC AAAACTTAGATGTGTGTTTGAATTGCCAGCAGAGAATGATAAACCACATGATGTAGAAATAAATAAAATT ATATCCACAACTGCATCAAAGACAGAAACACCAATAGTGTCTAAGTCTCTGAGTTCTTCTTTGGATGACA CCGAAGTTAAGAAGGTTATGGAAGAATGTAAGAGGCTGCAAGGTGAAGTTCAGAGGCTACGGGAGGAGAA CAAGCAGTTCAAGGAAGAAGATGGACTGCGGATGAGGAAGACAGTGCAGAGCAACAGCCCCATTTCAGCA TTAGCCCCAACTGGGAAGGAAGAAGGCCTTAGCACCCGGCTCTTGGCTCTGGTGGTTTTGTTCTTTATCG TTGGTGTAATTATTGGGAAGATTGCCTTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200517 protein sequence
Red=Cloning site Green=Tags(s) MAKVEQVLSLEPQHELKFRGPFTDVVTTNLKLGNPTDRNVCFKVKTTAPRRYCVRPNSGIIDAGASINVS VMLQPFDYDPNEKSKHKFMVQSMFAPTDTSDMEAVWKEAKPEDLMDSKLRCVFELPAENDKPHDVEINKI ISTTASKTETPIVSKSLSSSLDDTEVKKVMEECKRLQGEVQRLREENKQFKEEDGLRMRKTVQSNSPISA LAPTGKEEGLSTRLLALVVLFFIVGVIIGKIAL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_004738 |
ORF Size | 729 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_004738.1, NM_004738.2, NM_004738.3, NM_004738.4, NP_004729.1 |
RefSeq Size | 7949 bp |
RefSeq ORF | 732 bp |
Locus ID | 9217 |
Cytogenetics | 20q13.32 |
Domains | MSP_domain |
Protein Families | Transmembrane |
MW | 27.2 kDa |
Gene Summary | The protein encoded by this gene is a type IV membrane protein found in plasma and intracellular vesicle membranes. The encoded protein is found as a homodimer and as a heterodimer with VAPA. This protein also can interact with VAMP1 and VAMP2 and may be involved in vesicle trafficking. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC117187 | VAPB (untagged)-Human VAMP (vesicle-associated membrane protein)-associated protein B and C (VAPB), transcript variant 1 |
USD 310.00 |
|
RG200517 | VAPB (GFP-tagged) - Human VAMP (vesicle-associated membrane protein)-associated protein B and C (VAPB), transcript variant 1 |
USD 460.00 |
|
RC200517L1 | Lenti ORF clone of Human VAMP (vesicle-associated membrane protein)-associated protein B and C (VAPB), transcript variant 1, Myc-DDK-tagged |
USD 620.00 |
|
RC200517L2 | Lenti ORF clone of Human VAMP (vesicle-associated membrane protein)-associated protein B and C (VAPB), transcript variant 1, mGFP tagged |
USD 768.00 |
|
RC200517L3 | Lenti ORF clone of Human VAMP (vesicle-associated membrane protein)-associated protein B and C (VAPB), transcript variant 1, Myc-DDK-tagged |
USD 620.00 |
|
RC200517L4 | Lenti ORF clone of Human VAMP (vesicle-associated membrane protein)-associated protein B and C (VAPB), transcript variant 1, mGFP tagged |
USD 768.00 |
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