ANT 1 (PRPF6) (NM_012469) Human Tagged ORF Clone

CAT#: RC200527

PRPF6 (Myc-DDK-tagged)-Human PRP6 pre-mRNA processing factor 6 homolog (S. cerevisiae) (PRPF6)

Product Images

Western blot validation of overexpression lysate (Cat# LY415739) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200527 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified PRPF6 protein (Cat# TP300527). The protein was produced from HEK293T cells transfected with PRPF6 cDNA clone (Cat# RC200527) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PRPF6
• Synonyms: ANT-1; ANT1; C20orf14; hPrp6; Prp6; RP60; SNRNP102; TOM; U5-102K
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200527 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACAAGAAGAAGAAACCGTTCCTAGGGATGCCCGCGCCCCTCGGCTACGTGCCGGGGCTGGGCCGGG
  GCGCCACTGGCTTCACCACGCGGTCAGACATTGGGCCCGCCCGTGATGCAAATGACCCTGTGGATGATCG
  CCATGCACCCCCAGGCAAGAGAACCGTTGGGGACCAGATGAAGAAAAATCAGGCTGCTGACGATGACGAC
  GAGGATCTAAATGACACCAATTACGATGAGTTTAATGGCTATGCTGGGAGCCTCTTCTCAAGTGGACCCT
  ACGAGAAAGATGATGAGGAAGCAGATGCTATCTATGCAGCCCTGGATAAAAGGATGGATGAAAGAAGAAA
  AGAAAGACGGGAGCAAAGGGAGAAAGAAGAAATAGAGAAATATCGTATGGAACGCCCCAAAATCCAACAG
  CAGTTCTCAGACCTCAAGAGGAAGTTGGCAGAAGTCACAGAAGAAGAGTGGCTGAGCATCCCCGAGGTTG
  GCGATGCCAGAAATAAACGTCAGCGGAACCCACGCTATGAGAAGCTGACCCCTGTTCCTGACAGTTTCTT
  TGCCAAACATTTACAGACCGGAGAGAACCATACCTCAGTGGATCCCCGACAAACTCAATTTGGAGGTCTT
  AACACACCCTATCCAGGTGGACTAAACACTCCATACCCAGGTGGAATGACGCCAGGACTGATGACACCTG
  GCACAGGTGAGCTGGACATGAGGAAGATTGGCCAAGCGAGGAACACTCTGATGGACATGAGGCTGAGCCA
  GGTGTCTGACTCCGTGAGTGGACAGACCGTCGTTGACCCCAAAGGCTACCTGACGGATTTAAATTCCATG
  ATCCCGACACACGGAGGAGACATCAATGATATCAAGAAGGCGCGACTGCTCCTCAAGTCTGTTCGGGAGA
  CGAACCCTCATCACCCGCCAGCCTGGATTGCATCAGCCCGCCTGGAAGAAGTCACTGGGAAGCTACAAGT
  AGCTCGGAACCTTATCATGAAGGGGACGGAGATGTGCCCCAAGAGTGAAGATGTCTGGCTGGAAGCAGCC
  AGGTTGCAGCCTGGGGACACAGCCAAGGCCGTGGTAGCCCAAGCTGTCCGTCATCTCCCACAGTCTGTCA
  GGATTTACATCAGAGCCGCAGAGCTGGAAACGGACATTCGTGCAAAGAAGCGGGTTCTTCGGAAAGCCCT
  CGAGCATGTTCCAAACTCGGTTCGCTTGTGGAAAGCAGCCGTTGAGCTGGAAGAACCTGAAGATGCTAGA
  ATCATGCTGAGCCGAGCTGTGGAGTGCTGCCCCACCAGCGTGGAGCTCTGGCTTGCTCTGGCAAGGCTGG
  AGACCTATGAAAATGCCCGCAAGGTCTTGAACAAGGCGCGGGAGAACATTCCTACAGACCGACATATCTG
  GATCACGGCTGCTAAGCTGGAGGAAGCCAATGGGAACACGCAGATGGTGGAGAAGATCATCGACCGAGCC
  ATCACCTCGCTGCGGGCCAACGGTGTGGAGATCAACCGTGAGCAGTGGATCCAGGATGCCGAGGAATGTG
  ACAGGGCTGGGAGTGTGGCCACCTGCCAGGCCGTCATGCGTGCCGTGATTGGGATTGGGATTGAGGAGGA
  AGATCGGAAGCATACCTGGATGGAGGATGCTGACAGTTGTGTAGCCCACAATGCCCTGGAGTGTGCACGA
  GCCATCTACGCCTACGCCCTGCAGGTGTTCCCCAGCAAGAAGAGTGTGTGGCTGCGCGCCGCGTACTTCG
  AGAAGAACCATGGCACTCGGGAGTCCCTGGAAGCACTCCTGCAGAGGGCTGTGGCCCACTGCCCCAAAGC
  AGAGGTGCTGTGGCTCATGGGCGCCAAGTCCAAGTGGCTGGCAGGGGATGTGCCTGCAGCAAGGAGCATC
  CTGGCCCTGGCCTTCCAGGCCAACCCCAACAGTGAGGAGATCTGGCTGGCAGCCGTGAAGCTGGAGTCCG
  AGAATGATGAGTACGAGCGGGCCCGGAGGCTGCTGGCCAAGGCGCGGAGCAGTGCCCCCACCGCCCGGGT
  GTTCATGAAGTCTGTGAAGCTGGAGTGGGTGCAAGACAACATCAGGGCAGCCCAAGATCTGTGCGAGGAG
  GCCCTGCGGCACTATGAGGACTTCCCCAAGCTGTGGATGATGAAGGGGCAGATCGAGGAGCAGAAGGAGA
  TGATGGAGAAGGCGCGGGAAGCCTATAACCAGGGGTTGAAGAAGTGTCCCCACTCCACACCCCTGTGGCT
  TTTGCTCTCTCGGCTGGAGGAGAAGATTGGGCAGCTTACTCGAGCACGGGCCATTTTGGAAAAGTCTCGT
  CTGAAGAACCCAAAGAACCCTGGGCTGTGGTTGGAGTCCGTGCGGCTGGAGTACCGTGCGGGGCTGAAGA
  ACATCGCAAATACACTCATGGCCAAGGCGCTGCAGGAGTGCCCCAACTCCGGTATCCTGTGGTCTGAGGC
  CATCTTCCTCGAGGCAAGGCCCCAGAGGAGGACCAAGAGCGTGGATGCCCTGAAGAAGTGTGAGCATGAC
  CCCCATGTGCTCCTGGCCGTGGCCAAGCTGTTTTGGAGTCAGCGGAAGATCACCAAGGCCAGGGAGTGGT
  TCCACCGCACTGTGAAGATTGACTCGGACCTGGGGGATGCCTGGGCCTTCTTCTACAAGTTTGAGCTGCA
  GCATGGCACTGAGGAGCAGCAGGAGGAGGTGAGGAAGCGCTGTGAGAGTGCAGAGCCTCGGCATGGGGAG
  CTGTGGTGCGCCGTGTCCAAGGACATCGCCAACTGGCAGAAGAAGATCGGGGACATCCTTAGGCTGGTGG
  CCGGCCGCATCAAGAACACCTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200527 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MNKKKKPFLGMPAPLGYVPGLGRGATGFTTRSDIGPARDANDPVDDRHAPPGKRTVGDQMKKNQAADDDD
  EDLNDTNYDEFNGYAGSLFSSGPYEKDDEEADAIYAALDKRMDERRKERREQREKEEIEKYRMERPKIQQ
  QFSDLKRKLAEVTEEEWLSIPEVGDARNKRQRNPRYEKLTPVPDSFFAKHLQTGENHTSVDPRQTQFGGL
  NTPYPGGLNTPYPGGMTPGLMTPGTGELDMRKIGQARNTLMDMRLSQVSDSVSGQTVVDPKGYLTDLNSM
  IPTHGGDINDIKKARLLLKSVRETNPHHPPAWIASARLEEVTGKLQVARNLIMKGTEMCPKSEDVWLEAA
  RLQPGDTAKAVVAQAVRHLPQSVRIYIRAAELETDIRAKKRVLRKALEHVPNSVRLWKAAVELEEPEDAR
  IMLSRAVECCPTSVELWLALARLETYENARKVLNKARENIPTDRHIWITAAKLEEANGNTQMVEKIIDRA
  ITSLRANGVEINREQWIQDAEECDRAGSVATCQAVMRAVIGIGIEEEDRKHTWMEDADSCVAHNALECAR
  AIYAYALQVFPSKKSVWLRAAYFEKNHGTRESLEALLQRAVAHCPKAEVLWLMGAKSKWLAGDVPAARSI
  LALAFQANPNSEEIWLAAVKLESENDEYERARRLLAKARSSAPTARVFMKSVKLEWVQDNIRAAQDLCEE
  ALRHYEDFPKLWMMKGQIEEQKEMMEKAREAYNQGLKKCPHSTPLWLLLSRLEEKIGQLTRARAILEKSR
  LKNPKNPGLWLESVRLEYRAGLKNIANTLMAKALQECPNSGILWSEAIFLEARPQRRTKSVDALKKCEHD
  PHVLLAVAKLFWSQRKITKAREWFHRTVKIDSDLGDAWAFFYKFELQHGTEEQQEEVRKRCESAEPRHGE
  LWCAVSKDIANWQKKIGDILRLVAGRIKNTF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012469
• ORF Size: 2823 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012469.1, NM_012469.2, NM_012469.3, NP_036601.2
• RefSeq Size: 3120 bp
• RefSeq ORF: 2826 bp
• Locus ID: 24148
• Domains: TPR, HAT
• Protein Families: Druggable Genome
• Protein Pathways: Spliceosome
• MW: 106.9 kDa
• Gene Summary: The protein encoded by this gene appears to be involved in pre-mRNA splicing, possibly acting as a bridging factor between U5 and U4/U6 snRNPs in formation of the spliceosome. The encoded protein also can bind androgen receptor, providing a link between transcriptional activation and splicing. [provided by RefSeq, Jul 2008]