ANT 1 (PRPF6) (NM_012469) Human Tagged ORF Clone
CAT#: RC200527
PRPF6 (Myc-DDK-tagged)-Human PRP6 pre-mRNA processing factor 6 homolog (S. cerevisiae) (PRPF6)
"NM_012469" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | PRPF6 |
Synonyms | ANT-1; ANT1; C20orf14; hPrp6; Prp6; RP60; SNRNP102; TOM; U5-102K |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC200527 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACAAGAAGAAGAAACCGTTCCTAGGGATGCCCGCGCCCCTCGGCTACGTGCCGGGGCTGGGCCGGG GCGCCACTGGCTTCACCACGCGGTCAGACATTGGGCCCGCCCGTGATGCAAATGACCCTGTGGATGATCG CCATGCACCCCCAGGCAAGAGAACCGTTGGGGACCAGATGAAGAAAAATCAGGCTGCTGACGATGACGAC GAGGATCTAAATGACACCAATTACGATGAGTTTAATGGCTATGCTGGGAGCCTCTTCTCAAGTGGACCCT ACGAGAAAGATGATGAGGAAGCAGATGCTATCTATGCAGCCCTGGATAAAAGGATGGATGAAAGAAGAAA AGAAAGACGGGAGCAAAGGGAGAAAGAAGAAATAGAGAAATATCGTATGGAACGCCCCAAAATCCAACAG CAGTTCTCAGACCTCAAGAGGAAGTTGGCAGAAGTCACAGAAGAAGAGTGGCTGAGCATCCCCGAGGTTG GCGATGCCAGAAATAAACGTCAGCGGAACCCACGCTATGAGAAGCTGACCCCTGTTCCTGACAGTTTCTT TGCCAAACATTTACAGACCGGAGAGAACCATACCTCAGTGGATCCCCGACAAACTCAATTTGGAGGTCTT AACACACCCTATCCAGGTGGACTAAACACTCCATACCCAGGTGGAATGACGCCAGGACTGATGACACCTG GCACAGGTGAGCTGGACATGAGGAAGATTGGCCAAGCGAGGAACACTCTGATGGACATGAGGCTGAGCCA GGTGTCTGACTCCGTGAGTGGACAGACCGTCGTTGACCCCAAAGGCTACCTGACGGATTTAAATTCCATG ATCCCGACACACGGAGGAGACATCAATGATATCAAGAAGGCGCGACTGCTCCTCAAGTCTGTTCGGGAGA CGAACCCTCATCACCCGCCAGCCTGGATTGCATCAGCCCGCCTGGAAGAAGTCACTGGGAAGCTACAAGT AGCTCGGAACCTTATCATGAAGGGGACGGAGATGTGCCCCAAGAGTGAAGATGTCTGGCTGGAAGCAGCC AGGTTGCAGCCTGGGGACACAGCCAAGGCCGTGGTAGCCCAAGCTGTCCGTCATCTCCCACAGTCTGTCA GGATTTACATCAGAGCCGCAGAGCTGGAAACGGACATTCGTGCAAAGAAGCGGGTTCTTCGGAAAGCCCT CGAGCATGTTCCAAACTCGGTTCGCTTGTGGAAAGCAGCCGTTGAGCTGGAAGAACCTGAAGATGCTAGA ATCATGCTGAGCCGAGCTGTGGAGTGCTGCCCCACCAGCGTGGAGCTCTGGCTTGCTCTGGCAAGGCTGG AGACCTATGAAAATGCCCGCAAGGTCTTGAACAAGGCGCGGGAGAACATTCCTACAGACCGACATATCTG GATCACGGCTGCTAAGCTGGAGGAAGCCAATGGGAACACGCAGATGGTGGAGAAGATCATCGACCGAGCC ATCACCTCGCTGCGGGCCAACGGTGTGGAGATCAACCGTGAGCAGTGGATCCAGGATGCCGAGGAATGTG ACAGGGCTGGGAGTGTGGCCACCTGCCAGGCCGTCATGCGTGCCGTGATTGGGATTGGGATTGAGGAGGA AGATCGGAAGCATACCTGGATGGAGGATGCTGACAGTTGTGTAGCCCACAATGCCCTGGAGTGTGCACGA GCCATCTACGCCTACGCCCTGCAGGTGTTCCCCAGCAAGAAGAGTGTGTGGCTGCGCGCCGCGTACTTCG AGAAGAACCATGGCACTCGGGAGTCCCTGGAAGCACTCCTGCAGAGGGCTGTGGCCCACTGCCCCAAAGC AGAGGTGCTGTGGCTCATGGGCGCCAAGTCCAAGTGGCTGGCAGGGGATGTGCCTGCAGCAAGGAGCATC CTGGCCCTGGCCTTCCAGGCCAACCCCAACAGTGAGGAGATCTGGCTGGCAGCCGTGAAGCTGGAGTCCG AGAATGATGAGTACGAGCGGGCCCGGAGGCTGCTGGCCAAGGCGCGGAGCAGTGCCCCCACCGCCCGGGT GTTCATGAAGTCTGTGAAGCTGGAGTGGGTGCAAGACAACATCAGGGCAGCCCAAGATCTGTGCGAGGAG GCCCTGCGGCACTATGAGGACTTCCCCAAGCTGTGGATGATGAAGGGGCAGATCGAGGAGCAGAAGGAGA TGATGGAGAAGGCGCGGGAAGCCTATAACCAGGGGTTGAAGAAGTGTCCCCACTCCACACCCCTGTGGCT TTTGCTCTCTCGGCTGGAGGAGAAGATTGGGCAGCTTACTCGAGCACGGGCCATTTTGGAAAAGTCTCGT CTGAAGAACCCAAAGAACCCTGGGCTGTGGTTGGAGTCCGTGCGGCTGGAGTACCGTGCGGGGCTGAAGA ACATCGCAAATACACTCATGGCCAAGGCGCTGCAGGAGTGCCCCAACTCCGGTATCCTGTGGTCTGAGGC CATCTTCCTCGAGGCAAGGCCCCAGAGGAGGACCAAGAGCGTGGATGCCCTGAAGAAGTGTGAGCATGAC CCCCATGTGCTCCTGGCCGTGGCCAAGCTGTTTTGGAGTCAGCGGAAGATCACCAAGGCCAGGGAGTGGT TCCACCGCACTGTGAAGATTGACTCGGACCTGGGGGATGCCTGGGCCTTCTTCTACAAGTTTGAGCTGCA GCATGGCACTGAGGAGCAGCAGGAGGAGGTGAGGAAGCGCTGTGAGAGTGCAGAGCCTCGGCATGGGGAG CTGTGGTGCGCCGTGTCCAAGGACATCGCCAACTGGCAGAAGAAGATCGGGGACATCCTTAGGCTGGTGG CCGGCCGCATCAAGAACACCTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200527 protein sequence
Red=Cloning site Green=Tags(s) MNKKKKPFLGMPAPLGYVPGLGRGATGFTTRSDIGPARDANDPVDDRHAPPGKRTVGDQMKKNQAADDDD EDLNDTNYDEFNGYAGSLFSSGPYEKDDEEADAIYAALDKRMDERRKERREQREKEEIEKYRMERPKIQQ QFSDLKRKLAEVTEEEWLSIPEVGDARNKRQRNPRYEKLTPVPDSFFAKHLQTGENHTSVDPRQTQFGGL NTPYPGGLNTPYPGGMTPGLMTPGTGELDMRKIGQARNTLMDMRLSQVSDSVSGQTVVDPKGYLTDLNSM IPTHGGDINDIKKARLLLKSVRETNPHHPPAWIASARLEEVTGKLQVARNLIMKGTEMCPKSEDVWLEAA RLQPGDTAKAVVAQAVRHLPQSVRIYIRAAELETDIRAKKRVLRKALEHVPNSVRLWKAAVELEEPEDAR IMLSRAVECCPTSVELWLALARLETYENARKVLNKARENIPTDRHIWITAAKLEEANGNTQMVEKIIDRA ITSLRANGVEINREQWIQDAEECDRAGSVATCQAVMRAVIGIGIEEEDRKHTWMEDADSCVAHNALECAR AIYAYALQVFPSKKSVWLRAAYFEKNHGTRESLEALLQRAVAHCPKAEVLWLMGAKSKWLAGDVPAARSI LALAFQANPNSEEIWLAAVKLESENDEYERARRLLAKARSSAPTARVFMKSVKLEWVQDNIRAAQDLCEE ALRHYEDFPKLWMMKGQIEEQKEMMEKAREAYNQGLKKCPHSTPLWLLLSRLEEKIGQLTRARAILEKSR LKNPKNPGLWLESVRLEYRAGLKNIANTLMAKALQECPNSGILWSEAIFLEARPQRRTKSVDALKKCEHD PHVLLAVAKLFWSQRKITKAREWFHRTVKIDSDLGDAWAFFYKFELQHGTEEQQEEVRKRCESAEPRHGE LWCAVSKDIANWQKKIGDILRLVAGRIKNTF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_012469 |
ORF Size | 2823 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_012469.1, NM_012469.2, NM_012469.3, NP_036601.2 |
RefSeq Size | 3120 bp |
RefSeq ORF | 2826 bp |
Locus ID | 24148 |
Domains | TPR, HAT |
Protein Families | Druggable Genome |
Protein Pathways | Spliceosome |
MW | 106.9 kDa |
Gene Summary | The protein encoded by this gene appears to be involved in pre-mRNA splicing, possibly acting as a bridging factor between U5 and U4/U6 snRNPs in formation of the spliceosome. The encoded protein also can bind androgen receptor, providing a link between transcriptional activation and splicing. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC115369 | PRPF6 (untagged)-Human PRP6 pre-mRNA processing factor 6 homolog (S. cerevisiae) (PRPF6) |
USD 310.00 |
|
RG200527 | PRPF6 (GFP-tagged) - Human PRP6 pre-mRNA processing factor 6 homolog (S. cerevisiae) (PRPF6) |
USD 940.00 |
|
RC200527L3 | Lenti ORF clone of Human PRP6 pre-mRNA processing factor 6 homolog (S. cerevisiae) (PRPF6), Myc-DDK-tagged |
USD 1,050.00 |
|
RC200527L4 | Lenti ORF clone of Human PRP6 pre-mRNA processing factor 6 homolog (S. cerevisiae) (PRPF6), mGFP tagged |
USD 1,050.00 |
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