PAN2 (NM_014871) Human Tagged ORF Clone

CAT#: RC200573

PAN2 (Myc-DDK-tagged)-Human PAN2 poly(A) specific ribonuclease subunit homolog (S. cerevisiae) (PAN2), transcript variant 3

Product Images

Western blot validation of overexpression lysate (Cat# LY402390) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200573 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified PAN2 protein (Cat# TP300573). The protein was produced from HEK293T cells transfected with PAN2 cDNA clone (Cat# RC200573) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PAN2
• Synonyms: USP52
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200573 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACTTTGAGGGTCTGGACCCTGGACTGGCAGAATATGCCCCAGCCATGCATTCTGCCCTGGACCCTG
  TCTTGGATGCCCACCTGAACCCAAATCTGCTACAGAATGTGGAGCTGGACCCAGAGGGAGTGGCCTTGGA
  GGCTCTTCCCGTCCAGGAATCAGTGCACATAATGGAAGGTGTCTACTCTGAATTGCACAGCGTGGTGGCT
  GAAGTGGGTGTACCTGTTTCCGTCTCCCACTTTGACTTGCACGAGGAGATGCTGTGGGTGGGGAGCCACG
  GGGGCCATGCCACTTCATTTTTTGGCCCAGCCTTGGAGCGCTACTCATCCTTTCAAGTCAATGGCAGTGA
  TGATATTCGGCAGATCCAGAGCCTGGAGAATGGTATCCTTTTTCTCACCAAGAACAACCTCAAGTATATG
  GCCCGTGGGGGCCTCATTATATTTGATTACCTGCTGGATGAGAATGAGGATATGCACAGTCTCCTACTGA
  CTGACAGCAGCACTCTACTCGTTGGTGGGCTGCAGAATCACATACTAGAGATTGATCTTAACACTGTCCA
  GGAGACTCAGAAGTATGCAGTAGAGACGCCTGGAGTCACCATCATGAGACAGACAAATCGCTTCTTCTTC
  TGCGGCCACACGTCTGGCAAGGTTTCCCTGAGAGACCTCCGTACTTTTAAGGTGGAACATGAGTTTGATG
  CCTTCTCAGGAAGTCTGTCAGACTTTGATGTGCATGGCAACCTGCTAGCTGCCTGTGGCTTCTCCAGCCG
  CCTCACTGGCCTGGCCTGCGACCGTTTCCTCAAGGTGTATGATTTGCGCATGATGCGTGCCATCACACCA
  CTTCAAGTACATGTGGATCCTGCCTTCTTGCGCTTCATTCCTACATATACTTCTCGTCTTGCTATCATCT
  CTCAGTCAGGGCAGTGCCAATTCTGTGAACCCACAGGCCTGGCCAACCCAGCCGATATCTTTCATGTGAA
  TCCTGTGGGGCCTCTGCTAATGACATTTGATGTGTCAGCCAGCAAGCAGGCTCTGGCCTTTGGGGATTCT
  GAGGGCTGTGTGCACCTCTGGACTGATTCCCCGGAGCCTTCCTTCAACCCCTACTCCCGTGAGACTGAGT
  TTGCTTTGCCGTGTCTCGTGGACTCACTGCCTCCTCTGGACTGGAGCCAGGACCTGCTGCCTCTTTCCCT
  CATCCCTGTCCCACTCACCACTGACACACTTCTCTCTGATTGGCCTGCTGCCAACTCTGCTCCAGCTCCC
  AGGCGAGCACCACCCGTGGATGCAGAGATTCTGCGCACCATGAAGAAGGTGGGCTTCATTGGCTATGCGC
  CCAATCCCCGCACCAGGCTGCGCAATCAGATACCCTACAGACTCAAGGAGTCAGACAGTGAATTTGACAG
  CTTCAGCCAGGTCACTGAGTCACCAGTAGGACGAGAAGAGGAACCACATCTCCACATGGTTTCTAAGAAA
  TACCGCAAGGTGACCATCAAATATTCCAAGCTAGGGCTGGAGGACTTTGACTTCAAACACTACAATAAGA
  CCTTGTTTGCTGGATTAGAGCCCCACATTCCCAACGCCTACTGTAACTGCATGATCCAGGTGCTCTATTT
  CCTGGAGCCTGTACGCTGTCTAATTCAAAACCACCTTTGCCAGAAGGAGTTCTGTCTGGCATGTGAGCTG
  GGCTTCCTGTTTCACATGTTGGACCTCTCTCGTGGTGACCCTTGCCAGGGCAATAATTTTCTTCGGGCAT
  TCCGTACTATTCCTGAGGCCTCAGCCCTCGGTCTAATCCTGGCTGACTCAGATGAGGCCTCAGGCAAGGG
  CAATCTGGCCAGGCTCATTCAGAGGTGGAATCGCTTCATTCTCACTCAACTGCATCAAGATATGCAGGAG
  CTGGAAATACCACAGGCTTATCGAGGTGCTGGAGGCAGCAGCTTTTGCTCATCGGGGGACTCTGTTATTG
  GGCAGCTCTTCAGCTGTGAGATGGAGAACTGCAGCCTCTGCCGCTGTGGCAGTGAGACCGTGCGAGCCTC
  ATCCACTCTGCTTTTCACACTCTCCTACCCTGATGATAAAACTGGGAAGAACTATGACTTTGCTCAGGTG
  CTGAAGCGAAGCATCTGCCTGGACCAGAATACACAGGCCTGGTGTGACACCTGTGAAAAGTACCAGCCCA
  CGATTCAGACCCGCAACATCCGCCATCTGCCAGATATTCTTGTCATCAATTGTGAGGTGAACAGCTCAAA
  AGAGGCTGATTTCTGGAGAATGCAGGCTGAGGTTGCCTTCAAGATGGCAGTAAAGAAACACGGTGGGGAA
  ATCTCCAAGAACAAGGAATTTGCTTTGGCTGATTGGAAGGAACTAGGGAGTCCAGAGGGTGTGCTGGTGT
  GTCCCTCCATTGAGGAGTTGAAGAACGTCTGGCTTCCTTTCTCCATTCGCATGAAGATGACCAAAAACAA
  AGGGCTGGATGTTTGCAATTGGACTGATGGGGATGAGATGCAGTGGGGCCCAGCCAGGGCAGAGGAGGAG
  CATGGTGTCTATGTGTATGACCTGATGGCTACTGTGGTACACATCCTGGACTCACGCACAGGGGGCAGCC
  TGGTGGCTCACATCAAAGTTGGAGAGACCTACCACCAGCGCAAGGAGGGCGTTACTCACCAGCAGTGGTA
  TCTGTTCAATGACTTTCTTATTGAACCTATTGATAAGCATGAAGCTGTGCAGTTTGACATGAATTGGAAA
  GTACCTGCAATCCTTTATTATGTCAAACGGAATCTCAATTCCAGATACAACCTGAACATCAAGAACCCTA
  TTGAGGCAAGTGTCTTGCTGGCTGAAGCCTCGCTGGCACGGAAGCAGCGGAAAACACATACTACCTTTAT
  TCCACTGATGCTGAATGAGATGCCACAGATTGGGGACCTGGTGGGTCTGGATGCTGAGTTTGTCACCCTT
  AATGAGGAGGAAGCAGAGTTACGCAGTGATGGTACCAAGTCTACCATTAAACCAAGCCAGATGTCAGTAG
  CCAGGATTACCTGTGTTCGGGGCCAGGGACCCAATGAGGGTATCCCCTTCATTGATGACTACATCTCTAC
  CCAGGAGCAGGTGGTGGATTACTTGACTCAATACTCGGGTATAAAGCCTGGTGACCTCGATGCCAAAATT
  TCCTCCAAGCACCTAACAACTCTCAAGTCTACCTACTTAAAGCTTCGTTTTCTCATTGACATTGGAGTCA
  AGTTTGTGGGTCATGGCCTGCAGAAGGACTTCCGGGTCATCAACCTGATGGTGCCCAAGGACCAAGTCCT
  TGACACTGTCTACCTGTTCCATATGCCCCGAAAACGAATGATTTCCCTGCGATTCCTTGCTTGGTACTTT
  CTGGACCTGAAGATTCAAGGGGAAACCCATGACAGTATTGAGGATGCCCGCACAGCCCTTCAGCTGTACC
  GAAAGTATCTGGAGCTAAGCAAAAATGGCACTGAGCCTGAGTCTTTCCACAAGGTGCTCAAGGGTCTTTA
  TGAGAAGGGCAGAAAGATGGACTGGAAGGTGCCTGAGCCTGAGGGCCAAACAAGTCCCAAGAATGCAGCT
  GTCTTCTCCTCAGTGCTGGCGCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200573 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MNFEGLDPGLAEYAPAMHSALDPVLDAHLNPNLLQNVELDPEGVALEALPVQESVHIMEGVYSELHSVVA
  EVGVPVSVSHFDLHEEMLWVGSHGGHATSFFGPALERYSSFQVNGSDDIRQIQSLENGILFLTKNNLKYM
  ARGGLIIFDYLLDENEDMHSLLLTDSSTLLVGGLQNHILEIDLNTVQETQKYAVETPGVTIMRQTNRFFF
  CGHTSGKVSLRDLRTFKVEHEFDAFSGSLSDFDVHGNLLAACGFSSRLTGLACDRFLKVYDLRMMRAITP
  LQVHVDPAFLRFIPTYTSRLAIISQSGQCQFCEPTGLANPADIFHVNPVGPLLMTFDVSASKQALAFGDS
  EGCVHLWTDSPEPSFNPYSRETEFALPCLVDSLPPLDWSQDLLPLSLIPVPLTTDTLLSDWPAANSAPAP
  RRAPPVDAEILRTMKKVGFIGYAPNPRTRLRNQIPYRLKESDSEFDSFSQVTESPVGREEEPHLHMVSKK
  YRKVTIKYSKLGLEDFDFKHYNKTLFAGLEPHIPNAYCNCMIQVLYFLEPVRCLIQNHLCQKEFCLACEL
  GFLFHMLDLSRGDPCQGNNFLRAFRTIPEASALGLILADSDEASGKGNLARLIQRWNRFILTQLHQDMQE
  LEIPQAYRGAGGSSFCSSGDSVIGQLFSCEMENCSLCRCGSETVRASSTLLFTLSYPDDKTGKNYDFAQV
  LKRSICLDQNTQAWCDTCEKYQPTIQTRNIRHLPDILVINCEVNSSKEADFWRMQAEVAFKMAVKKHGGE
  ISKNKEFALADWKELGSPEGVLVCPSIEELKNVWLPFSIRMKMTKNKGLDVCNWTDGDEMQWGPARAEEE
  HGVYVYDLMATVVHILDSRTGGSLVAHIKVGETYHQRKEGVTHQQWYLFNDFLIEPIDKHEAVQFDMNWK
  VPAILYYVKRNLNSRYNLNIKNPIEASVLLAEASLARKQRKTHTTFIPLMLNEMPQIGDLVGLDAEFVTL
  NEEEAELRSDGTKSTIKPSQMSVARITCVRGQGPNEGIPFIDDYISTQEQVVDYLTQYSGIKPGDLDAKI
  SSKHLTTLKSTYLKLRFLIDIGVKFVGHGLQKDFRVINLMVPKDQVLDTVYLFHMPRKRMISLRFLAWYF
  LDLKIQGETHDSIEDARTALQLYRKYLELSKNGTEPESFHKVLKGLYEKGRKMDWKVPEPEGQTSPKNAA
  VFSSVLAL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014871
• ORF Size: 3594 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014871.2, NM_014871.3, NM_014871.4, NP_055686.2
• RefSeq Size: 5289 bp
• RefSeq ORF: 3597 bp
• Locus ID: 9924
• Domains: UCH
• Protein Families: Protease
• MW: 135 kDa
• Gene Summary: This gene encodes a deadenylase that functions as the catalytic subunit of the polyadenylate binding protein dependent poly(A) nuclease complex. The encoded protein is a magnesium dependent 3' to 5' exoribonuclease that is involved in the degradation of cytoplasmic mRNAs. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]