KPNB1 (NM_002265) Human Tagged ORF Clone

CAT#: RC200659

KPNB1 (Myc-DDK-tagged)-Human karyopherin (importin) beta 1 (KPNB1)

Product Images

Western blot validation of overexpression lysate (Cat# LY419417) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200659 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KPNB1
• Synonyms: IMB1; Impnb; IPO1; IPOB; NTF97
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200659 representing NM_002265
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCTGATCACCATTCTCGAGAAGACCGTGTCTCCCGATCGGCTGGAGCTGGAAGCGGCGCAGAAGT
  TCCTGGAGCGTGCGGCCGTGGAGAACCTGCCCACTTTCCTTGTGGAACTGTCCAGAGTGCTGGCAAATCC
  AGGAAACAGTCAGGTTGCCAGAGTTGCAGCTGGTCTACAAATCAAGAACTCTTTGACATCTAAAGATCCA
  GATATCAAGGCACAATATCAGCAGAGGTGGCTTGCTATTGATGCTAATGCTCGACGAGAAGTCAAGAACT
  ATGTTTTGCAGACATTGGGTACAGAAACTTACCGGCCTAGTTCTGCCTCACAGTGTGTGGCTGGTATTGC
  TTGTGCAGAGATCCCAGTAAACCAGTGGCCAGAACTCATTCCTCAGCTGGTGGCCAATGTCACAAACCCC
  AACAGCACAGAGCACATGAAGGAGTCGACATTGGAAGCCATCGGTTATATTTGCCAAGATATAGACCCAG
  AGCAGCTACAAGATAAATCCAATGAGATTCTGACTGCCATAATCCAGGGGATGAGGAAAGAAGAGCCTAG
  TAATAATGTGAAGCTAGCTGCTACGAATGCACTCCTGAACTCATTGGAGTTCACCAAAGCAAACTTTGAT
  AAAGAGTCTGAAAGGCACTTTATTATGCAGGTGGTCTGTGAAGCCACACAGTGTCCAGATACGAGGGTAC
  GAGTGGCTGCTTTACAGAATCTGGTGAAGATAATGTCCTTATATTATCAGTACATGGAGACATATATGGG
  TCCTGCTCTTTTTGCAATCACAATCGAAGCAATGAAAAGTGACATTGATGAGGTGGCTTTACAAGGGATA
  GAATTCTGGTCCAATGTCTGTGATGAGGAAATGGATTTGGCCATTGAAGCTTCAGAGGCAGCAGAACAAG
  GACGGCCCCCTGAGCACACCAGCAAGTTTTATGCGAAGGGAGCACTACAGTATCTGGTTCCAATCCTCAC
  ACAGACACTAACTAAACAGGACGAAAATGATGATGACGATGACTGGAACCCCTGCAAAGCAGCAGGGGTG
  TGCCTCATGCTTCTGGCCACCTGCTGTGAAGATGACATTGTCCCACATGTCCTCCCCTTCATTAAAGAAC
  ACATCAAGAACCCAGATTGGCGGTACCGGGATGCAGCAGTGATGGCTTTTGGTTGTATCTTGGAAGGACC
  AGAGCCCAGTCAGCTCAAACCACTAGTTATACAGGCTATGCCCACCCTAATAGAATTAATGAAAGACCCC
  AGTGTAGTTGTTCGAGATACAGCTGCATGGACTGTAGGCAGAATTTGTGAGCTGCTTCCTGAAGCTGCCA
  TCAATGATGTCTACTTGGCTCCCCTGCTACAGTGTCTGATTGAGGGTCTCAGTGCTGAACCCAGAGTGGC
  TTCAAATGTGTGCTGGGCTTTCTCCAGTCTGGCTGAAGCTGCTTATGAAGCTGCAGACGTTGCTGATGAT
  CAGGAAGAACCAGCTACTTACTGCTTATCTTCTTCATTTGAACTCATAGTTCAGAAGCTCCTAGAGACTA
  CAGACAGACCTGATGGACACCAGAACAACCTGAGGAGTTCTGCATATGAATCTCTGATGGAAATTGTGAA
  AAACAGTGCCAAGGATTGTTATCCTGCTGTCCAGAAAACGACTTTGGTCATCATGGAACGACTGCAACAG
  GTTCTTCAGATGGAGTCACATATCCAGAGCACATCCGATAGAATCCAGTTCAATGACCTTCAGTCTTTAC
  TCTGTGCAACTCTTCAGAATGTTCTTCGGAAAGTGCAACATCAAGATGCTTTGCAGATCTCTGATGTGGT
  TATGGCCTCCCTGTTAAGGATGTTCCAAAGCACAGCTGGGTCTGGGGGAGTACAAGAGGATGCCCTGATG
  GCAGTTAGCACACTGGTGGAAGTGTTGGGTGGTGAATTCCTCAAGTACATGGAGGCCTTTAAACCCTTCC
  TGGGCATTGGATTAAAAAATTATGCTGAATACCAGGTTTGTTTGGCAGCTGTGGGCTTAGTGGGAGACTT
  GTGCCGTGCCCTGCAATCCAACATCATACCTTTCTGTGACGAGGTGATGCAGCTGCTTCTGGAAAATTTG
  GGGAATGAGAACGTCCACAGGTCTGTGAAGCCGCAGATTCTGTCAGTGTTTGGTGATATTGCCCTTGCTA
  TTGGAGGAGAGTTTAAAAAATACTTAGAGGTTGTATTGAATACTCTTCAGCAGGCCTCCCAAGCCCAGGT
  GGACAAGTCAGACTATGACATGGTGGATTATCTGAATGAGCTAAGGGAAAGCTGCTTGGAAGCCTATACT
  GGAATCGTCCAGGGATTAAAGGGGGATCAGGAGAACGTACACCCGGATGTGATGCTGGTACAACCCAGAG
  TAGAATTTATTCTGTCTTTCATTGACCACATTGCTGGAGATGAGGATCACACAGATGGAGTAGTAGCTTG
  TGCTGCTGGACTAATAGGGGACTTATGTACAGCATTTGGGAAGGATGTACTGAAATTAGTAGAAGCTAGG
  CCAATGATCCATGAATTGTTAACTGAAGGGCGGAGATCGAAGACTAACAAAGCAAAAACCCTTGCTACAT
  GGGCAACAAAAGAACTGAGGAAACTGAAGAACCAAGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200659 representing NM_002265
  Red=Cloning site Green=Tags(s)
  
  MELITILEKTVSPDRLELEAAQKFLERAAVENLPTFLVELSRVLANPGNSQVARVAAGLQIKNSLTSKDP
  DIKAQYQQRWLAIDANARREVKNYVLQTLGTETYRPSSASQCVAGIACAEIPVNQWPELIPQLVANVTNP
  NSTEHMKESTLEAIGYICQDIDPEQLQDKSNEILTAIIQGMRKEEPSNNVKLAATNALLNSLEFTKANFD
  KESERHFIMQVVCEATQCPDTRVRVAALQNLVKIMSLYYQYMETYMGPALFAITIEAMKSDIDEVALQGI
  EFWSNVCDEEMDLAIEASEAAEQGRPPEHTSKFYAKGALQYLVPILTQTLTKQDENDDDDDWNPCKAAGV
  CLMLLATCCEDDIVPHVLPFIKEHIKNPDWRYRDAAVMAFGCILEGPEPSQLKPLVIQAMPTLIELMKDP
  SVVVRDTAAWTVGRICELLPEAAINDVYLAPLLQCLIEGLSAEPRVASNVCWAFSSLAEAAYEAADVADD
  QEEPATYCLSSSFELIVQKLLETTDRPDGHQNNLRSSAYESLMEIVKNSAKDCYPAVQKTTLVIMERLQQ
  VLQMESHIQSTSDRIQFNDLQSLLCATLQNVLRKVQHQDALQISDVVMASLLRMFQSTAGSGGVQEDALM
  AVSTLVEVLGGEFLKYMEAFKPFLGIGLKNYAEYQVCLAAVGLVGDLCRALQSNIIPFCDEVMQLLLENL
  GNENVHRSVKPQILSVFGDIALAIGGEFKKYLEVVLNTLQQASQAQVDKSDYDMVDYLNELRESCLEAYT
  GIVQGLKGDQENVHPDVMLVQPRVEFILSFIDHIAGDEDHTDGVVACAAGLIGDLCTAFGKDVLKLVEAR
  PMIHELLTEGRRSKTNKAKTLATWATKELRKLKNQA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002265
• ORF Size: 2628 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002265.1, NM_002265.2, NM_002265.3, NM_002265.4, NM_002265.5, NP_002256.2
• RefSeq Size: 4205 bp
• RefSeq ORF: 2631 bp
• Locus ID: 3837
• Cytogenetics: 17q21.32
• Domains: Armadillo_seg, IBN_NT
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• MW: 97 kDa
• Gene Summary: 'Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2013]'