DDOST (NM_005216) Human Tagged ORF Clone
CAT#: RC200672L2
- LentiORF®
Lenti ORF clone of Human dolichyl-diphosphooligosaccharide--protein glycosyltransferase (DDOST), mGFP tagged
"NM_005216" in other vectors (6)
Product Images
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Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | mGFP |
Symbol | DDOST |
Synonyms | AGER1; CDG1R; GATD6; OKSWcl45; OST; OST48; WBP1 |
Vector | pLenti-C-mGFP |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC200672).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NM_005216 |
ORF Size | 1368 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_005216.4, NP_005207.2 |
RefSeq Size | 2144 bp |
RefSeq ORF | 1371 bp |
Locus ID | 1650 |
Cytogenetics | 1p36.12 |
Domains | DDOST_48kD |
Protein Families | Transmembrane |
Protein Pathways | Metabolic pathways, N-Glycan biosynthesis |
MW | 50.7 kDa |
Gene Summary | 'This gene encodes a component of the oligosaccharyltransferase complex which catalyzes the transfer of high-mannose oligosaccharides to asparagine residues on nascent polypeptides in the lumen of the rough endoplasmic reticulum. The protein complex co-purifies with ribosomes. The product of this gene is also implicated in the processing of advanced glycation endproducts (AGEs), which form from non-enzymatic reactions between sugars and proteins or lipids and are associated with aging and hyperglycemia. [provided by RefSeq, Jul 2008]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC116849 | DDOST (untagged)-Human dolichyl-diphosphooligosaccharide--protein glycosyltransferase (DDOST) |
USD 310.00 |
|
RC200672 | DDOST (Myc-DDK-tagged)-Human dolichyl-diphosphooligosaccharide--protein glycosyltransferase (DDOST) |
USD 420.00 |
|
RG200672 | DDOST (GFP-tagged) - Human dolichyl-diphosphooligosaccharide--protein glycosyltransferase (DDOST) |
USD 460.00 |
|
RC200672L1 | Lenti ORF clone of Human dolichyl-diphosphooligosaccharide--protein glycosyltransferase (DDOST), Myc-DDK-tagged |
USD 768.00 |
|
RC200672L3 | Lenti ORF clone of Human dolichyl-diphosphooligosaccharide--protein glycosyltransferase (DDOST), Myc-DDK-tagged |
USD 620.00 |
|
RC200672L4 | Lenti ORF clone of Human dolichyl-diphosphooligosaccharide--protein glycosyltransferase (DDOST), mGFP tagged |
USD 620.00 |
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