MAD3 (MXD3) (NM_031300) Human Tagged ORF Clone
CAT#: RC200747
MXD3 (Myc-DDK-tagged)-Human MAX dimerization protein 3 (MXD3), transcript variant 1
"NM_031300" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | MXD3 |
Synonyms | BHLHC13; MAD3; MYX |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC200747 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAACCCTTGGCCAGCAACATCCAGGTCCTGCTGCAGGCGGCCGAGTTCCTGGAGCGCCGTGAGAGAG AGGCCGAGCATGGTTATGCGTCCCTGTGCCCGCATCGCAGTCCAGGCCCCATCCACAGGAGGAAGAAGCG ACCCCCCCAGGCTCCTGGCGCGCAGGACAGCGGGCGGTCAGTGCACAATGAACTGGAGAAGCGCAGGAGG GCCCAGTTGAAGCGGTGCCTGGAGCGGCTGAAGCAGCAGATGCCCCTGGGGGCCGACTGTGCCCGGTACA CCACGCTGAGCCTGCTGCGCCGTGCCAGGATGCACATCCAGAAGCTGGAGGATCAGGAGCAGCGGGCCCG ACAGCTCAAGGAGAGGCTGCGCAGCAAGCAGCAGAGCCTGCAGCGGCAGCTGGAGCAGCTCCGGGGGCTG GCAGGGGCGGCCGAGCGGGAGCGGCTGCGGGCGGACAGTCTGGACTCCTCAGGCCTCTCCTCTGAGCGCT CAGACTCAGACCAAGAGGAGCTGGAGGTGGATGTGGAGAGCCTGGTGTTTGGGGGTGAGGCCGAGCTGCT GCGGGGCTTCGTCGCCGGCCAGGAGCACAGCTACTCGCACGGCGGCGGCGCCTGGCTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200747 protein sequence
Red=Cloning site Green=Tags(s) MEPLASNIQVLLQAAEFLERREREAEHGYASLCPHRSPGPIHRRKKRPPQAPGAQDSGRSVHNELEKRRR AQLKRCLERLKQQMPLGADCARYTTLSLLRRARMHIQKLEDQEQRARQLKERLRSKQQSLQRQLEQLRGL AGAAERERLRADSLDSSGLSSERSDSDQEELEVDVESLVFGGEAELLRGFVAGQEHSYSHGGGAWL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_031300 |
ORF Size | 618 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_031300.1, NM_031300.2, NM_031300.3, NP_112590.1 |
RefSeq Size | 1483 bp |
RefSeq ORF | 621 bp |
Locus ID | 83463 |
Domains | HLH |
Protein Families | Druggable Genome, Transcription Factors |
MW | 23.5 kDa |
Gene Summary | This gene encodes a member of the Myc superfamily of basic helix-loop-helix leucine zipper transcriptional regulators. The encoded protein forms a heterodimer with the cofactor MAX which binds specific E-box DNA motifs in the promoters of target genes and regulates their transcription. Disruption of the MAX-MXD3 complex is associated with uncontrolled cell proliferation and tumorigenesis. Transcript variants of this gene encoding different isoforms have been described. [provided by RefSeq, Dec 2008] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC319057 | MXD3 (untagged)-Human MAX dimerization protein 3 (MXD3), transcript variant 1 |
USD 310.00 |
|
RG200747 | MXD3 (GFP-tagged) - Human MAX dimerization protein 3 (MXD3), transcript variant 1 |
USD 460.00 |
|
RC200747L3 | Lenti ORF clone of Human MAX dimerization protein 3 (MXD3), transcript variant 1, Myc-DDK-tagged |
USD 620.00 |
|
RC200747L4 | Lenti ORF clone of Human MAX dimerization protein 3 (MXD3), transcript variant 1, mGFP tagged |
USD 620.00 |
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