FGFBP1 (NM_005130) Human Tagged ORF Clone

CAT#: RC200819

FGFBP1 (Myc-DDK-tagged)-Human fibroblast growth factor binding protein 1 (FGFBP1)

Product Images

Western blot validation of overexpression lysate (Cat# LY417486) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200819 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FGFBP1
• Synonyms: FGF-BP; FGF-BP1; FGFBP; FGFBP-1; HBP17
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200819 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGATCTGTAGCCTCACCCTGCTCTCCTTCCTCCTACTGGCTGCTCAGGTGCTCCTGGTGGAGGGGA
  AAAAAAAAGTGAAGAATGGACTTCACAGCAAAGTGGTCTCAGAACAAAAGGACACTCTGGGCAACACCCA
  GATTAAGCAGAAAAGCAGGCCCGGGAACAAAGGCAAGTTTGTCACCAAAGACCAAGCCAACTGCAGATGG
  GCTGCTACTGAGCAGGAGGAGGGCATCTCTCTCAAGGTTGAGTGCACTCAATTGGACCATGAATTTTCCT
  GTGTCTTTGCTGGCAATCCAACCTCATGCCTAAAGCTCAAGGATGAGAGAGTCTATTGGAAACAAGTTGC
  CCGGAATCTGCGCTCACAGAAAGACATCTGTAGATATTCCAAGACAGCTGTGAAAACCAGAGTGTGCAGA
  AAGGATTTTCCAGAATCCAGTCTTAAGCTAGTCAGCTCCACTCTATTTGGGAACACAAAGCCCAGGAAGG
  AGAAAACAGAGATGTCCCCCAGGGAGCACATCAAGGGCAAAGAGACCACCCCCTCTAGCCTAGCAGTGAC
  CCAGACCATGGCCACCAAAGCTCCCGAGTGTGTGGAGGACCCAGATATGGCAAACCAGAGGAAGACTGCC
  CTGGAGTTCTGTGGAGAGACTTGGAGCTCTCTCTGCACATTCTTCCTCAGCATAGTGCAGGACACGTCAT
  GC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200819 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKICSLTLLSFLLLAAQVLLVEGKKKVKNGLHSKVVSEQKDTLGNTQIKQKSRPGNKGKFVTKDQANCRW
  AATEQEEGISLKVECTQLDHEFSCVFAGNPTSCLKLKDERVYWKQVARNLRSQKDICRYSKTAVKTRVCR
  KDFPESSLKLVSSTLFGNTKPRKEKTEMSPREHIKGKETTPSSLAVTQTMATKAPECVEDPDMANQRKTA
  LEFCGETWSSLCTFFLSIVQDTSC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005130
• ORF Size: 702 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005130.1, NM_005130.2, NM_005130.3, NM_005130.4, NP_005121.1
• RefSeq Size: 1369 bp
• RefSeq ORF: 705 bp
• Locus ID: 9982
• Protein Families: Secreted Protein
• MW: 26.3 kDa
• Gene Summary: This gene encodes a secreted fibroblast growth factor carrier protein. The encoded protein plays a critical role in cell proliferation, differentiation and migration by binding to fibroblast growth factors and potentiating their biological effects on target cells. The encoded protein may also play a role in tumor growth as an angiogenic switch molecule, and expression of this gene has been associated with several types of cancer including pancreatic and colorectal adenocarcinoma. A pseudogene of this gene is also located on the short arm of chromosome 4. [provided by RefSeq, Nov 2011]