ZBTB37 (NM_032522) Human Tagged ORF Clone

CAT#: RC200839

ZBTB37 (Myc-DDK-tagged)-Human zinc finger and BTB domain containing 37 (ZBTB37), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY410053) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200839 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZBTB37
• Synonyms: D430004I08Rik; ZNF908
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200839 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAAAGGTGGGAACATACAATTGGAGATTCCTGACTTCAGCAACTCTGTCCTGAGCCATCTAAACC
  AGTTGCGCATGCAGGGCCGTCTCTGTGATATTGTGGTCAATGTGCAAGGACAAGCTTTTCGGGCTCACAA
  AGTGGTGCTGGCTGCCAGCTCCCCCTATTTCCGGGATCACATGTCCTTGAATGAGATGAGTACAGTCTCC
  ATTTCAGTCATCAAGAACCCTACTGTTTTTGAACAGCTCCTTTCTTTCTGTTACACAGGGCGGATATGCC
  TGCAACTGGCAGATATCATCAGCTACCTAACAGCTGCCAGTTTTCTGCAAATGCAGCATATTATAGACAA
  ATGTACACAGATCCTGGAGGGCATTCATTTCAAAATTAATGTGGCTGAGGTTGAAGCAGAATTAAGTCAA
  ACAAGGACAAAGCATCAAGAGAGACCTCCAGAGTCTCACAGGGTTACACCAAATCTCAACCGCTCCCTTA
  GCCCACGACATAATACCCCAAAGGGAAACCGGCGAGGTCAGGTTAGTGCTGTGCTGGATATCAGAGAGCT
  AAGTCCTCCTGAGGAGTCCACCAGCCCTCAGATCATTGAACCAAGTTCTGATGTAGAGAGCCGGGAGCCC
  ATTCTTCGGATCAACCGAGCAGGACAGTGGTATGTGGAGACAGGAGTGGCGGACCGTGGGGGTCGGAGTG
  ATGATGAAGTTAGAGTTCTTGGAGCAGTACACATCAAAACTGAAAATCTGGAGGAGTGGCTTGGGCCTGA
  GAATCAGCCTTCTGGAGAAGATGGGAGTAGTGCAGAGGAAGTAACAGCCATGGTGATTGATACCACAGGC
  CATGGTTCTGTAGGACAGGAAAATTATACTTTAGGGTCTTCAGGAGCCAAGGTGGCTCGGCCAACAAGCA
  GTGAAGTTGACAGATTTAGCCCCTCCGGCAGTGTTGTTCCCTTGACAGAGAGACACAGAGCCAGAAGTGA
  GTCTCCTGGGAGAATGGATGAGCCTAAGCAACCCAGCTCCCAGGTATGGAGTTGTGGATTTAGAACTGCT
  TTGGTGGTTGGAGGAATTGCTACTGTGTATGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200839 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEKGGNIQLEIPDFSNSVLSHLNQLRMQGRLCDIVVNVQGQAFRAHKVVLAASSPYFRDHMSLNEMSTVS
  ISVIKNPTVFEQLLSFCYTGRICLQLADIISYLTAASFLQMQHIIDKCTQILEGIHFKINVAEVEAELSQ
  TRTKHQERPPESHRVTPNLNRSLSPRHNTPKGNRRGQVSAVLDIRELSPPEESTSPQIIEPSSDVESREP
  ILRINRAGQWYVETGVADRGGRSDDEVRVLGAVHIKTENLEEWLGPENQPSGEDGSSAEEVTAMVIDTTG
  HGSVGQENYTLGSSGAKVARPTSSEVDRFSPSGSVVPLTERHRARSESPGRMDEPKQPSSQVWSCGFRTA
  LVVGGIATVYE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032522
• ORF Size: 1083 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032522.1, NM_032522.2, NM_032522.3, NM_032522.4, NP_115911.1
• RefSeq Size: 1386 bp
• RefSeq ORF: 1086 bp
• Locus ID: 84614
• Protein Families: Transcription Factors
• MW: 39.8 kDa
• Gene Summary: May be involved in transcriptional regulation. [UniProtKB/Swiss-Prot Function]