DCUN1D5 (NM_032299) Human Tagged ORF Clone

CAT#: RC200870

DCUN1D5 (Myc-DDK-tagged)-Human DCN1, defective in cullin neddylation 1, domain containing 5 (S. cerevisiae) (DCUN1D5)

Product Images

Western blot validation of overexpression lysate (Cat# LY410241) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200870 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified DCUN1D5 protein (Cat# TP300870). The protein was produced from HEK293T cells transfected with DCUN1D5 cDNA clone (Cat# RC200870) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DCUN1D5
• Synonyms: SCCRO5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200870 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGGTGAAGAAGAAGAGAAAATCCCCTGGGGTGGCAGCAGCAGTAGCGGAAGACGGAGGCCTCAAAA
  AGTGTAAAATCTCCAGCTATTGCAGATCCCAACCCCCTGCTAGACTAATAAGTGGAGAGGAACATTTTTC
  AAGCAAGAAGTGCCTGGCTTGGTTTTATGAATATGCAGGTCCTGATGAAGTTGTAGGGCCAGAAGGAATG
  GAAAAATTTTGTGAAGACATTGGTGTTGAACCTGAAAATATTATTATGTTAGTTTTAGCGTGGAAATTGG
  AGGCTGAAAGCATGGGATTTTTTACCAAGGAAGAATGGTTAAAGGGAATGACTTCATTACAGTGTGACTG
  CACAGAAAAGTTACAAAACAAATTTGACTTTTTGCGCTCACAGTTGAATGATATTTCGTCATTTAAGAAT
  ATCTACAGATATGCCTTTGATTTTGCAAGGGATAAAGATCAGAGAAGCCTTGATATTGATACTGCTAAAT
  CTATGTTAGCTCTTCTGCTTGGGAGGACATGGCCACTGTTTTCAGTATTTTACCAGTACCTGGAGCAATC
  AAAGTATCGTGTTATGAACAAAGATCAATGGTACAATGTATTAGAATTCAGCAGAACAGTCCATGCTGAT
  CTTAGTAACTATGATGAAGATGGTGCTTGGCCTGTTCTTCTTGATGAATTTGTTGAGTGGCAAAAAGTCC
  GTCAGACATCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200870 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPVKKKRKSPGVAAAVAEDGGLKKCKISSYCRSQPPARLISGEEHFSSKKCLAWFYEYAGPDEVVGPEGM
  EKFCEDIGVEPENIIMLVLAWKLEAESMGFFTKEEWLKGMTSLQCDCTEKLQNKFDFLRSQLNDISSFKN
  IYRYAFDFARDKDQRSLDIDTAKSMLALLLGRTWPLFSVFYQYLEQSKYRVMNKDQWYNVLEFSRTVHAD
  LSNYDEDGAWPVLLDEFVEWQKVRQTS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032299
• ORF Size: 711 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032299.1, NM_032299.2, NM_032299.3, NP_115675.1
• RefSeq Size: 12732 bp
• RefSeq ORF: 714 bp
• Locus ID: 84259
• Domains: DUF298
• MW: 27.5 kDa