ABCB6 (NM_005689) Human Tagged ORF Clone

CAT#: RC200949

ABCB6 (Myc-DDK-tagged)-Human ATP-binding cassette, sub-family B (MDR/TAP), member 6 (ABCB6), nuclear gene encoding mitochondrial protein

Product Images

Western blot validation of overexpression lysate (Cat# LY417131) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200949 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ABCB6
• Synonyms: ABC; LAN; MTABC3; PRP; umat
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200949 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGACTGTGGGCAACTACTGCGAGGCCGAAGGGCCCGTGGGTCCGGCCTGGATGCAGGATGGCCTGA
  GTCCCTGCTTCTTCTTCACGCTCGTGCCCTCGACGCGGATGGCTCTAGGGACTCTGGCCTTGGTGCTGGC
  TCTTCCCTGCAGACGCCGGGAGCGGCCCGCTGGTGCTGATTCGCTGTCTTGGGGGGCCGGCCCTCGCATC
  TCTCCCTACGTGCTGCAGCTGCTTCTGGCCACACTTCAGGCGGCGCTGCCCCTGGCCGGCCTGGCTGGCC
  GGGTGGGCACTGCCCGGGGGGCCCCACTGCCAAGCTATCTACTTCTGGCCTCCGTGCTGGAGAGTCTGGC
  CGGCGCCTGTGGCCTGTGGCTGCTTGTCGTGGAGCGGAGCCAGGCACGGCAGCGTCTGGCAATGGGCATC
  TGGATCAAGTTCAGGCACAGCCCTGGTCTCCTGCTCCTCTGGACTGTGGCGTTTGCAGCTGAGAACTTGG
  CCCTGGTGTCTTGGAACAGCCCACAGTGGTGGTGGGCAAGGGCAGACTTGGGCCAGCAGGTTCAGTTTAG
  CCTGTGGGTGCTGCGGTATGTGGTCTCTGGAGGGCTGTTTGTCCTGGGTCTCTGGGCCCCTGGACTTCGT
  CCCCAGTCCTATACATTGCAGGTTCATGAAGAGGACCAAGATGTGGAAAGGAGCCAGGTTCGGTCAGCAG
  CCCAACAGTCTACCTGGCGAGATTTTGGCAGGAAGCTCCGCCTCCTGAGTGGCTACCTGTGGCCTCGAGG
  GAGTCCAGCTCTGCAGCTGGTGGTGCTCATCTGCCTGGGGCTCATGGGTTTGGAACGGGCACTCAATGTG
  TTGGTGCCTATATTCTATAGGAACATTGTGAACTTGCTGACTGAGAAGGCACCTTGGAACTCTCTGGCCT
  GGACTGTTACCAGTTACGTCTTCCTCAAGTTCCTCCAGGGGGGTGGCACTGGCAGTACAGGCTTCGTGAG
  CAACCTGCGCACCTTCCTGTGGATCCGGGTGCAGCAGTTCACGTCTCGGCGGGTGGAGCTGCTCATCTTC
  TCCCACCTGCACGAGCTCTCACTGCGCTGGCACCTGGGGCGCCGCACAGGGGAGGTGCTGCGGATCGCGG
  ATCGGGGCACATCCAGTGTCACAGGGCTGCTCAGCTACCTGGTGTTCAATGTCATCCCCACGCTGGCCGA
  CATCATCATTGGCATCATCTACTTCAGCATGTTCTTCAACGCCTGGTTTGGCCTCATTGTGTTCCTGTGC
  ATGAGTCTTTACCTCACCCTGACCATTGTGGTCACTGAGTGGAGAACCAAGTTTCGTCGTGCTATGAACA
  CACAGGAGAACGCTACCCGGGCACGAGCAGTGGACTCTCTGCTAAACTTCGAGACGGTGAAGTATTACAA
  CGCCGAGAGTTACGAAGTGGAACGCTATCGAGAGGCCATCATCAAATATCAGGGTTTGGAGTGGAAGTCG
  AGCGCTTCACTGGTTTTACTAAATCAGACCCAGAACCTGGTAATTGGGCTCGGGCTCCTCGCCGGCTCCC
  TGCTTTGCGCATACTTTGTCACTGAGCAGAAGCTACAGGTTGGGGACTATGTGCTCTTTGGCACCTACAT
  TATCCAGCTGTACATGCCCCTCAATTGGTTTGGCACCTACTACAGGATGATCCAGACCAACTTCATTGAC
  ATGGAGAACATGTTTGACTTGCTGAAAGAGGAGACAGAAGTGAAGGACCTTCCTGGAGCAGGGCCCCTTC
  GCTTTCAGAAGGGCCGTATTGAGTTTGAGAACGTGCACTTCAGCTATGCCGATGGGCGGGAGACTCTGCA
  GGACGTGTCTTTCACTGTGATGCCTGGACAGACACTTGCCCTGGTGGGCCCATCTGGGGCAGGGAAGAGC
  ACAATTTTGCGCCTGCTGTTTCGCTTCTACGACATCAGCTCTGGCTGCATCCGAATAGATGGGCAGGACA
  TTTCACAGGTGACCCAGGCCTCTCTCCGGTCTCACATTGGAGTTGTGCCCCAAGACACTGTCCTCTTTAA
  TGACACCATCGCCGACAATATCCGTTACGGCCGTGTCACAGCTGGGAATGATGAGGTGGAGGCTGCTGCT
  CAGGCTGCAGGCATCCATGATGCCATTATGGCTTTCCCTGAAGGGTACAGGACACAGGTGGGCGAGCGGG
  GACTGAAGCTGAGCGGCGGGGAGAAGCAGCGCGTCGCCATTGCCCGCACCATCCTCAAGGCTCCGGGCAT
  CATTCTGCTGGATGAGGCAACGTCAGCGCTGGATACATCTAATGAGAGGGCCATCCAGGCTTCTCTGGCC
  AAAGTCTGTGCCAACCGCACCACCATCGTAGTGGCACACAGGCTCTCAACTGTGGTCAATGCTGACCAGA
  TCCTCGTCATCAAGGATGGCTGCATCGTGGAGAGGGGACGACACGAGGCTCTGTTGTCCCGAGGTGGGGT
  GTATGCTGACATGTGGCAGCTGCAGCAGGGACAGGAAGAAACCTCTGAAGACACTAAGCCTCAGACCATG
  GAACGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200949 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVTVGNYCEAEGPVGPAWMQDGLSPCFFFTLVPSTRMALGTLALVLALPCRRRERPAGADSLSWGAGPRI
  SPYVLQLLLATLQAALPLAGLAGRVGTARGAPLPSYLLLASVLESLAGACGLWLLVVERSQARQRLAMGI
  WIKFRHSPGLLLLWTVAFAAENLALVSWNSPQWWWARADLGQQVQFSLWVLRYVVSGGLFVLGLWAPGLR
  PQSYTLQVHEEDQDVERSQVRSAAQQSTWRDFGRKLRLLSGYLWPRGSPALQLVVLICLGLMGLERALNV
  LVPIFYRNIVNLLTEKAPWNSLAWTVTSYVFLKFLQGGGTGSTGFVSNLRTFLWIRVQQFTSRRVELLIF
  SHLHELSLRWHLGRRTGEVLRIADRGTSSVTGLLSYLVFNVIPTLADIIIGIIYFSMFFNAWFGLIVFLC
  MSLYLTLTIVVTEWRTKFRRAMNTQENATRARAVDSLLNFETVKYYNAESYEVERYREAIIKYQGLEWKS
  SASLVLLNQTQNLVIGLGLLAGSLLCAYFVTEQKLQVGDYVLFGTYIIQLYMPLNWFGTYYRMIQTNFID
  MENMFDLLKEETEVKDLPGAGPLRFQKGRIEFENVHFSYADGRETLQDVSFTVMPGQTLALVGPSGAGKS
  TILRLLFRFYDISSGCIRIDGQDISQVTQASLRSHIGVVPQDTVLFNDTIADNIRYGRVTAGNDEVEAAA
  QAAGIHDAIMAFPEGYRTQVGERGLKLSGGEKQRVAIARTILKAPGIILLDEATSALDTSNERAIQASLA
  KVCANRTTIVVAHRLSTVVNADQILVIKDGCIVERGRHEALLSRGGVYADMWQLQQGQEETSEDTKPQTM
  ER
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005689
• ORF Size: 2526 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005689.1, NM_005689.2, NM_005689.3, NP_005680.1
• RefSeq Size: 3021 bp
• RefSeq ORF: 2529 bp
• Locus ID: 10058
• Domains: ABC_membrane, ABC_tran, AAA
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: ABC transporters
• MW: 93.9 kDa
• Gene Summary: This gene encodes a member of the ATP-binding cassette (ABC) transporter superfamily. ABC proteins transport various molecules across extra- and intra-cellular membranes. This protein is a member of the heavy metal importer subfamily and plays a role in porphyrin transport. This gene is the molecular basis of the Langereis (Lan) blood group antigen and mutations in this gene underlie familial pseudohyperkalemia and dyschromatosis universalis hereditaria. [provided by RefSeq, Mar 2017]