GDAP1L1 (NM_024034) Human Tagged ORF Clone
CAT#: RC200976
GDAP1L1 (Myc-DDK-tagged)-Human ganglioside-induced differentiation-associated protein 1-like 1 (GDAP1L1)
"NM_024034" in other vectors (5)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | GDAP1L1 |
Synonyms | dJ881L22.1; dJ995J12.1.1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC200976 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGACCCCCAACAATCTGACCCCCACCAACTGCAGCTGGTGGCCCATCTCCGCGCTGGAGAGCGATG CGGCCAAGCCAGCGGAGGCCCCCGACGCTCCCGAGGCGGCCAGCCCCGCCCATTGGCCCAGGGAGAGCCT GGTTCTGTACCACTGGACCCAGTCCTTCAGCTCGCAGAAGGTGCGGCTGGTGATCGCCGAGAAGGGCCTG GTGTGCGAGGAGCGGGACGTGAGCCTGCCACAGAGCGAGCACAAGGAGCCCTGGTTCATGCGGCTCAACC TGGGCGAGGAGGTGCCCGTCATCATCCACCGCGACAACATCATCAGTGACTATGACCAGATCATTGACTA TGTGGAGCGCACCTTCACAGGAGAGCACGTGGTGGCCCTGATGCCCGAGGTGGGCAGCCTGCAGCACGCA CGGGTGCTGCAGTACCGGGAGCTGCTGGACGCACTGCCCATGGATGCCTACACGCATGGCTGCATCCTGC ATCCCGAGCTCACCACCGACTCCATGATCCCCAAGTACGCCACGGCCGAGATCCGCAGACATTTAGCCAA TGCCACCACGGACCTCATGAAACTGGACCATGAAGAGGAGCCCCAGCTCTCCGAGCCCTACCTTTCTAAA CAAAAGAAGCTCATGGCCAAGATCTTGGAGCATGATGATGTGAGCTACCTGAAGAAGATCCTCGGGGAAC TGGCCATGGTGCTGGACCAGATTGAGGCGGAGCTGGAGAAGAGGAAGCTGGAGAACGAGGGGCAGAAATG CGAGCTGTGGCTCTGTGGCTGTGCCTTCACCCTCGCTGATGTCCTCCTGGGAGCCACCCTGCACCGCCTC AAGTTCCTGGGACTGTCCAAGAAATACTGGGAAGATGGCAGCCGGCCCAACCTGCAGTCCTTCTTTGAGA GGGTCCAGAGACGCTTTGCCTTCCGGAAAGTCCTGGGTGACATCCACACCACCCTGCTGTCGGCCGTCAT CCCCAATGCTTTCCGGCTGGTCAAGAGGAAACCCCCATCCTTCTTCGGGGCGTCCTTCCTCATGGGCTCC CTGGGTGGGATGGGCTACTTTGCCTACTGGTACCTCAAGAAAAAATACATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200976 protein sequence
Red=Cloning site Green=Tags(s) MATPNNLTPTNCSWWPISALESDAAKPAEAPDAPEAASPAHWPRESLVLYHWTQSFSSQKVRLVIAEKGL VCEERDVSLPQSEHKEPWFMRLNLGEEVPVIIHRDNIISDYDQIIDYVERTFTGEHVVALMPEVGSLQHA RVLQYRELLDALPMDAYTHGCILHPELTTDSMIPKYATAEIRRHLANATTDLMKLDHEEEPQLSEPYLSK QKKLMAKILEHDDVSYLKKILGELAMVLDQIEAELEKRKLENEGQKCELWLCGCAFTLADVLLGATLHRL KFLGLSKKYWEDGSRPNLQSFFERVQRRFAFRKVLGDIHTTLLSAVIPNAFRLVKRKPPSFFGASFLMGS LGGMGYFAYWYLKKKYI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_024034 |
ORF Size | 1101 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_024034.1, NM_024034.2, NM_024034.3, NM_024034.4, NP_076939.3 |
RefSeq Size | 2798 bp |
RefSeq ORF | 1104 bp |
Locus ID | 78997 |
Protein Families | Transmembrane |
MW | 42 kDa |
Gene Summary | The ganglioside GD3 synthase causes cell differentiation with neurite sprouting when transfected into the mouse neuroblastoma cell line Neuro2a. After differentiation, the expression of several genes is upregulated, including one that encodes a protein termed ganglioside-induced differentiation-associated protein 1 (Gdap1). A similar gene was found in humans, and mutations in the human gene are associated with Charcot-Marie-Tooth type 4A disease. The protein encoded by this gene is similar in sequence to the human GDAP1 protein. Several transcript variants encoding different isoforms, as well as a noncoding transcript variant, have been found for this gene. [provided by RefSeq, Feb 2012] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC112289 | GDAP1L1 (untagged)-Human ganglioside-induced differentiation-associated protein 1-like 1 (GDAP1L1) |
USD 310.00 |
|
SC320647 | GDAP1L1 (untagged)-Human ganglioside-induced differentiation-associated protein 1-like 1 (GDAP1L1) |
USD 310.00 |
|
RG200976 | GDAP1L1 (GFP-tagged) - Human ganglioside-induced differentiation-associated protein 1-like 1 (GDAP1L1) |
USD 460.00 |
|
RC200976L3 | Lenti ORF clone of Human ganglioside-induced differentiation-associated protein 1-like 1 (GDAP1L1), Myc-DDK-tagged |
USD 620.00 |
|
RC200976L4 | Lenti ORF clone of Human ganglioside-induced differentiation-associated protein 1-like 1 (GDAP1L1), mGFP tagged |
USD 620.00 |
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