Mutarotase (GALM) (NM_138801) Human Tagged ORF Clone

CAT#: RC200995

GALM (Myc-DDK-tagged)-Human galactose mutarotase (aldose 1-epimerase) (GALM)

Product Images

Western blot validation of overexpression lysate (Cat# LY408487) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200995 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified GALM protein (Cat# TP300995). The protein was produced from HEK293T cells transfected with GALM cDNA clone (Cat# RC200995) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GALM
• Synonyms: BLOCK25; GLAT; HEL-S-63p; IBD1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200995 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTCGGTGACCAGGGCCGTGTTTGGAGAGCTGCCCTCGGGAGGAGGGACAGTGGAGAAGTTCCAGC
  TGCAGTCAGACCTCTTGAGAGTGGACATCATCTCCTGGGGCTGCACGATCACAGCCCTAGAGGTCAAAGA
  CAGGCAGGGGAGAGCCTCGGACGTGGTGCTTGGCTTCGCCGAGTTGGAAGGATACCTCCAAAAGCAGCCA
  TACTTTGGAGCAGTTATTGGGAGGGTGGCCAACCGAATCGCCAAAGGAACCTTCAAGGTGGATGGGAAGG
  AGTATCACCTGGCCATTAACAAGGAACCCAACAGTCTGCATGGAGGAGTCAGAGGGTTTGATAAAGTGCT
  CTGGACCCCTCGGGTGCTGTCAAATGGCGTCCAGTTCTCGCGCATCAGTCCAGATGGTGAAGAAGGCTAC
  CCCGGAGAGTTAAAAGTCTGGGTGACATACACCCTGGATGGCGGAGAGCTCATAGTCAACTACAGAGCAC
  AAGCCAGTCAGGCCACACCAGTCAACCTGACCAACCATTCTTACTTCAACCTGGCAGGCCAGGCTTCCCC
  AAATATAAATGACCATGAAGTCACCATAGAAGCGGATACTTATTTGCCTGTGGATGAAACCCTGATTCCT
  ACAGGAGAAGTTGCCCCAGTGCAAGGCACTGCATTCGACCTGAGAAAGCCAGTGGAGCTTGGAAAACACC
  TGCAGGACTTCCATCTCAATGGTTTTGACCACAATTTCTGTCTGAAGGGATCTAAAGAAAAGCATTTTTG
  TGCAAGGGTGCATCATGCTGCAAGCGGGCGGGTACTAGAAGTATACACCACCCAGCCCGGGGTCCAGTTT
  TACACGGGCAACTTCCTGGATGGCACATTAAAGGGCAAGAATGGAGCTGTCTATCCCAAGCACTCCGGTT
  TCTGCCTGGAGACTCAGAACTGGCCTGATGCAGTCAATCAGCCCCGCTTCCCTCCTGTGCTGCTGAGGCC
  TGGTGAGGAGTATGACCACACCACCTGGTTCAAGTTTTCTGTGGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200995 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MASVTRAVFGELPSGGGTVEKFQLQSDLLRVDIISWGCTITALEVKDRQGRASDVVLGFAELEGYLQKQP
  YFGAVIGRVANRIAKGTFKVDGKEYHLAINKEPNSLHGGVRGFDKVLWTPRVLSNGVQFSRISPDGEEGY
  PGELKVWVTYTLDGGELIVNYRAQASQATPVNLTNHSYFNLAGQASPNINDHEVTIEADTYLPVDETLIP
  TGEVAPVQGTAFDLRKPVELGKHLQDFHLNGFDHNFCLKGSKEKHFCARVHHAASGRVLEVYTTQPGVQF
  YTGNFLDGTLKGKNGAVYPKHSGFCLETQNWPDAVNQPRFPPVLLRPGEEYDHTTWFKFSVA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_138801
• ORF Size: 1026 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_138801.1, NM_138801.2, NP_620156.1
• RefSeq Size: 2483 bp
• RefSeq ORF: 1029 bp
• Locus ID: 130589
• Domains: Aldose_epim
• Protein Pathways: Glycolysis / Gluconeogenesis
• MW: 37.8 kDa
• Gene Summary: This gene encodes an enzyme that catalyzes the epimerization of hexose sugars such as glucose and galactose. The encoded protein is expressed in the cytoplasm and has a preference for galactose. The encoded protein may be required for normal galactose metabolism by maintaining the equilibrium of alpha and beta anomers of galactose. [provided by RefSeq, Mar 2009]