Syntaxin 18 (STX18) (NM_016930) Human Tagged ORF Clone

CAT#: RC201016

STX18 (Myc-DDK-tagged)-Human syntaxin 18 (STX18)

Product Images

Western blot validation of overexpression lysate (Cat# LY413750) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201016 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: STX18
• Synonyms: Ufe1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201016 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGTGGACATCACGCTGCTATTCCGGGCCAGCGTCAAGACCGTGAAGACGCGGAACAAGGCGCTGG
  GAGTGGCGGTGGGCGGCGGGGTCGATGGCAGCCGGGACGAGCTGTTCCGCCGGAGCCCCCGGCCCAAGGG
  CGACTTCTCCAGCCGGGCCCGCGAAGTGATTTCTCACATTGGCAAACTGAGAGATTTTCTTCTGGAACAC
  AGGAAAGATTATATTAATGCTTATAGCCATACCATGTCTGAATATGGGAGGATGACAGACACAGAACGAG
  ACCAGATAGACCAGGATGCCCAGATATTCATGAGGACCTGTTCAGAAGCAATTCAGCAACTACGAACAGA
  AGCTCACAAGGAGATACATTCCCAGCAAGTGAAGGAGCACAGGACCGCTGTTTTGGATTTCATTGAAGAT
  TACTTGAAAAGAGTATGTAAACTTTACTCAGAACAGAGAGCCATCCGAGTTAAAAGAGTGGTGGATAAGA
  AAAGATTATCTAAGTTGGAACCAGAACCAAATACAAAGACAAGAGAATCCACATCTTCTGAGAAAGTTTC
  ACAGAGTCCTTCAAAAGACTCTGAAGAAAACCCTGCCACTGAAGAACGTCCAGAAAAAATTTTGGCTGAA
  ACACAACCTGAATTGGGAACGTGGGGAGATGGCAAAGGCGAAGATGAGTTATCCCCAGAAGAAATACAAA
  TGTTTGAACAGGAAAATCAGCGACTAATTGGTGAAATGAACAGCTTGTTTGATGAAGTGAGGCAAATCGA
  AGGGAGAGTGGTTGAGATTTCCAGACTCCAAGAGATATTCACGGAAAAGGTTTTGCAACAGGAAGCTGAG
  ATTGACAGCATTCACCAGTTAGTTGTGGGGGCAACTGAAAATATCAAGGAAGGCAACGAAGACATAAGAG
  AGGCCATTAAAAACAACGCTGGCTTCCGCGTGTGGATCCTCTTCTTCCTCGTGATGTGCTCCTTCTCCTT
  GCTCTTCCTCGACTGGTACGACAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201016 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAVDITLLFRASVKTVKTRNKALGVAVGGGVDGSRDELFRRSPRPKGDFSSRAREVISHIGKLRDFLLEH
  RKDYINAYSHTMSEYGRMTDTERDQIDQDAQIFMRTCSEAIQQLRTEAHKEIHSQQVKEHRTAVLDFIED
  YLKRVCKLYSEQRAIRVKRVVDKKRLSKLEPEPNTKTRESTSSEKVSQSPSKDSEENPATEERPEKILAE
  TQPELGTWGDGKGEDELSPEEIQMFEQENQRLIGEMNSLFDEVRQIEGRVVEISRLQEIFTEKVLQQEAE
  IDSIHQLVVGATENIKEGNEDIREAIKNNAGFRVWILFFLVMCSFSLLFLDWYDS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016930
• ORF Size: 1005 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016930.1, NM_016930.2, NM_016930.3, NP_058626.1
• RefSeq Size: 2162 bp
• RefSeq ORF: 1008 bp
• Locus ID: 53407
• Protein Families: Transmembrane
• Protein Pathways: SNARE interactions in vesicular transport
• MW: 38.7 kDa
• Gene Summary: This gene encodes a member of the syntaxin family of soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) which is part of a membrane tethering complex that includes other SNAREs and several peripheral membrane proteins, and is involved in vesicular transport between the endoplasmic reticulum (ER) and the Golgi complex. The encoded protein is important for the organization of the smooth, rough, and exit site ER subdomains. A pseudogene of this gene has been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2016]