C17orf81 (ELP5) (NM_203415) Human Tagged ORF Clone

CAT#: RC201035

ELP5 (Myc-DDK-tagged)-Human chromosome 17 open reading frame 81 (C17orf81), transcript variant 4

Product Images

Western blot validation of overexpression lysate (Cat# LY404351) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214461 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ELP5
• Synonyms: C17orf81; DERP6; HSPC002; MST071; MSTP071
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201035 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGCCATCAGAGGGCGCCAGAGCAGGGACCGGACGCGAGTTGGAGATGTTGGACTCGCTGTTGGCCT
  TGGGCGGCCTGGTGCTGCTTCGGGATTCCGTGGAGTGGGAGGGGCGCAGTCTCTTGAAGGCGCTTGTCAA
  GAAATCTGCACTGTGTGGGGAGCAAGTGCATATCCTGGGCTGTGAAGTGAGCGAGGAAGAGTTTCGTGAA
  GGTTTTGACTCTGATATCAACAATCGGCTGGTTTACCATGACTTCTTCAGAGACCCTCTCAACTGGTCAA
  AAACTGAGGAGGCCTTTCCTGGGGGGCCGCTGGGAGCCTTGAGAGCCATGTGCAAGAGGACAGATCCTGT
  TCCTGTCACCATTGCTCTCGATTCACTCAGCTGGCTGCTACTTCGCCTTCCCTGCACCACACTCTGCCAG
  GTCCTGCATGCTGTGAGCCATCAGGACTCTTGTCCTGGTGACAGCTCCTCAGTGGGGAAAGTGAGTGTGC
  TGGGCTTGCTACATGAAGAGCTTCATGGACCAGGCCCTGTGGGAGCTCTCAGCAGCCTTGCTCAGACTGA
  GGTGACCCTGGGCGGTACCATGGGCCAGGCCTCGGCCCACATCCTGTGTCGGAGGCCCCGACAGCGCCCA
  ACTGACCAGACTCAGTGGTTCTCCATCCTTCCGGACTTCAGCCTGGATCTCCAAGAGGGGCCCTCTGTAG
  AGTCCCAGCCCTACTCCGATCCTCATATACCCCCGGTGGATCCCACAACTCATTTGACCTTTAACCTTCA
  CCTGTCCAAGAAAGAGAGAGAAGCCAGAGATAGCCTGATCCTGCCCTTCCAGTTCAGTTCTGAAAAACAG
  CAGGCTCTCCTGCGGCCTAGGCCAGGGCAGGCTACCAGCCACATCTTCTATGAGCCAGATGCTTATTATG
  ACCTGGACCAAGAAGACCCAGATGACGACCTGGATATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201035 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTPSEGARAGTGRELEMLDSLLALGGLVLLRDSVEWEGRSLLKALVKKSALCGEQVHILGCEVSEEEFRE
  GFDSDINNRLVYHDFFRDPLNWSKTEEAFPGGPLGALRAMCKRTDPVPVTIALDSLSWLLLRLPCTTLCQ
  VLHAVSHQDSCPGDSSSVGKVSVLGLLHEELHGPGPVGALSSLAQTEVTLGGTMGQASAHILCRRPRQRP
  TDQTQWFSILPDFSLDLQEGPSVESQPYSDPHIPPVDPTTHLTFNLHLSKKEREARDSLILPFQFSSEKQ
  QALLRPRPGQATSHIFYEPDAYYDLDQEDPDDDLDI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_203415
• ORF Size: 948 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_203415.1, NM_203415.2, NP_981960.1
• RefSeq Size: 1322 bp
• RefSeq ORF: 951 bp
• Locus ID: 23587
• MW: 34.9 kDa