MYL9 (NM_181526) Human Tagged ORF Clone
CAT#: RC201080
MYL9 (Myc-DDK-tagged)-Human myosin, light chain 9, regulatory (MYL9), transcript variant 2
"NM_181526" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | MYL9 |
Synonyms | LC20; MLC-2C; MLC2; MRLC1; MYRL2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC201080 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCAGCAAGCGGGCCAAAGCCAAGACCACCAAGAAGCGGCCACAGCGGGCCACATCCAATGTCTTCG CAATGTTTGACCAGTCCCAGATCCAGGAGTTTAAGGAGGCTTTCAACATGATTGACCAGAACCGTGATGG CTTCATTGACAAGGAGGACCTGCACGACATGCTGGCCTCGCTGGGTTTCATCCATGAGGACCACCTCCGG GAGCTGCTCACCACCATGGGTGACCGCTTCACAGATGAGGAAGTGGACGAGATGTACCGGGAGGCACCCA TTGATAAGAAAGGCAACTTCAACTACGTGGAGTTCACCCGCATCCTCAAACATGGCGCCAAGGATAAAGA CGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC201080 protein sequence
Red=Cloning site Green=Tags(s) MSSKRAKAKTTKKRPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGFIHEDHLR ELLTTMGDRFTDEEVDEMYREAPIDKKGNFNYVEFTRILKHGAKDKDD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_181526 |
ORF Size | 354 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_181526.1, NM_181526.2, NP_852667.1 |
RefSeq Size | 1054 bp |
RefSeq ORF | 357 bp |
Locus ID | 10398 |
Protein Pathways | Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton, Tight junction, Vascular smooth muscle contraction |
MW | 13.9 kDa |
Gene Summary | Myosin, a structural component of muscle, consists of two heavy chains and four light chains. The protein encoded by this gene is a myosin light chain that may regulate muscle contraction by modulating the ATPase activity of myosin heads. The encoded protein binds calcium and is activated by myosin light chain kinase. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC319114 | MYL9 (untagged)-Human myosin, light chain 9, regulatory (MYL9), transcript variant 2 |
USD 420.00 |
|
RG201080 | MYL9 (GFP-tagged) - Human myosin, light chain 9, regulatory (MYL9), transcript variant 2 |
USD 460.00 |
|
RC201080L1 | Lenti ORF clone of Human myosin, light chain 9, regulatory (MYL9), transcript variant 2, Myc-DDK-tagged |
USD 768.00 |
|
RC201080L2 | Lenti ORF clone of Human myosin, light chain 9, regulatory (MYL9), transcript variant 2, mGFP tagged |
USD 620.00 |
|
RC201080L3 | Lenti ORF clone of Human myosin, light chain 9, regulatory (MYL9), transcript variant 2, Myc-DDK-tagged |
USD 620.00 |
|
RC201080L4 | Lenti ORF clone of Human myosin, light chain 9, regulatory (MYL9), transcript variant 2, mGFP tagged |
USD 620.00 |
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