SF3A1 (NM_005877) Human Tagged ORF Clone

CAT#: RC201098

SF3A1 (Myc-DDK-tagged)-Human splicing factor 3a, subunit 1, 120kDa (SF3A1), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY416998) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201098 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified SF3A1 protein (Cat# TP301098). The protein was produced from HEK293T cells transfected with SF3A1 cDNA clone (Cat# RC201098) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SF3A1
• Synonyms: PRP21; PRPF21; SAP114; SF3A120
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201098 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGGCCGGACCCGTGCAGGCGGTGCCCCCGCCGCCGCCCGTGCCCACGGAGCCCAAACAGCCCACAG
  AAGAAGAAGCATCTTCAAAGGAGGATTCTGCACCTTCTAAGCCAGTTGTGGGGATTATTTACCCTCCTCC
  AGAGGTCAGAAATATTGTTGACAAGACTGCCAGCTTTGTGGCCAGAAACGGGCCTGAATTTGAAGCTAGG
  ATCCGACAGAACGAGATCAACAACCCCAAGTTCAACTTTCTGAACCCCAATGACCCTTACCATGCCTACT
  ACCGCCACAAGGTCAGCGAGTTCAAGGAAGGGAAGGCTCAGGAGCCGTCCGCCGCCATCCCCAAGGTCAT
  GCAGCAGCAGCAGCAGACCACCCAGCAGCAGCTGCCCCAGAAGGTCCAAGCCCAAGTAATCCAAGAGACC
  ATCGTGCCCAAAGAGCCTCCTCCTGAGTTTGAGTTCATTGCTGATCCTCCCTCTATCTCAGCCTTCGACT
  TGGATGTGGTGAAGCTGACGGCTCAGTTTGTGGCCAGGAATGGGCGCCAGTTTCTGACCCAGCTGATGCA
  GAAAGAGCAGCGCAACTACCAGTTTGACTTTCTCCGCCCACAGCACAGCCTCTTCAACTACTTCACGAAG
  CTAGTGGAACAGTACACCAAGATCTTGATTCCACCCAAAGGTTTATTTTCAAAGCTCAAGAAAGAGGCTG
  AAAACCCCCGAGAAGTTTTGGATCAGGTGTGTTACCGAGTGGAATGGGCCAAATTCCAGGAACGTGAGAG
  GAAGAAGGAAGAAGAGGAGAAGGAGAAGGAGCGGGTGGCCTATGCTCAGATCGACTGGCATGATTTTGTG
  GTGGTGGAAACAGTGGACTTCCAACCCAATGAGCAAGGGAACTTCCCTCCCCCCACCACGCCAGAGGAGC
  TGGGGGCCCGAATCCTCATTCAGGAGCGCTATGAAAAGTTTGGGGAGAGTGAGGAAGTTGAGATGGAGGT
  CGAGTCTGATGAGGAGGATGACAAACAGGAGAAGGCGGAGGAGCCTCCTTCCCAGCTGGACCAGGACACC
  CAAGTACAAGATATGGATGAGGGTTCAGATGATGAAGAAGAAGGGCAGAAAGTGCCCCCACCCCCAGAGA
  CACCCATGCCTCCACCTCTGCCCCCAACTCCAGACCAAGTCATTGTCCGCAAGGATTATGATCCCAAAGC
  CTCCAAGCCCTTGCCTCCAGCCCCTGCTCCAGATGAGTATCTTGTGTCCCCCATTACTGGGGAGAAGATC
  CCCGCCAGCAAAATGCAGGAACACATGCGCATTGGACTTCTTGACCCTCGCTGGCTGGAGCAGCGGGATC
  GCTCCATCCGTGAGAAGCAGAGCGATGATGAGGTGTACGCACCAGGTCTGGATATTGAGAGCAGCTTGAA
  GCAGTTGGCTGAGCGGCGTACTGACATCTTCGGTGTAGAGGAAACAGCCATTGGTAAGAAGATCGGTGAG
  GAGGAGATCCAGAAGCCAGAGGAAAAGGTGACCTGGGATGGCCACTCAGGCAGCATGGCCCGGACCCAGC
  AGGCTGCCCAGGCCAACATCACCCTCCAGGAGCAGATTGAGGCCATTCACAAGGCCAAAGGCCTGGTGCC
  AGAGGATGACACTAAAGAGAAGATTGGCCCCAGCAAGCCCAATGAAATCCCTCAACAGCCACCGCCACCA
  TCTTCAGCCACCAACATCCCCAGCTCGGCTCCACCCATCACTTCAGTGCCCCGACCACCCACAATGCCAC
  CTCCAGTTCGTACTACAGTTGTCTCCGCAGTACCCGTCATGCCCCGGCCCCCAATGGCATCTGTGGTCCG
  GCTGCCCCCAGGCTCAGTGATCGCCCCCATGCCGCCCATCATCCACGCGCCCAGAATCAACGTGGTGCCC
  ATGCCTCCCTCGGCCCCTCCTATTATGGCCCCCCGCCCACCCCCCATGATTGTGCCAACAGCCTTTGTGC
  CTGCTCCACCTGTGGCACCTGTCCCAGCTCCAGCCCCAATGCCCCCTGTGCATCCCCCACCTCCCATGGA
  AGATGAGCCCACCTCCAAAAAACTGAAGACAGAGGACAGCCTCATGCCAGAGGAGGAGTTCCTGCGCAGA
  AACAAGGGTCCAGTGTCCATCAAAGTCCAGGTGCCCAACATGCAGGATAAGACGGAATGGAAACTGAATG
  GGCAGGTGCTGGTCTTCACCCTCCCACTCACGGACCAGGTCTCTGTCATTAAGGTGAAGATTCATGAAGC
  CACAGGCATGCCTGCAGGGAAACAGAAGCTACAGTATGAGGGTATCTTCATCAAAGATTCCAACTCACTG
  GCTTACTACAACATGGCCAATGGCGCAGTCATCCACCTGGCCCTCAAGGAGAGAGGCGGGAGGAAGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201098 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPAGPVQAVPPPPPVPTEPKQPTEEEASSKEDSAPSKPVVGIIYPPPEVRNIVDKTASFVARNGPEFEAR
  IRQNEINNPKFNFLNPNDPYHAYYRHKVSEFKEGKAQEPSAAIPKVMQQQQQTTQQQLPQKVQAQVIQET
  IVPKEPPPEFEFIADPPSISAFDLDVVKLTAQFVARNGRQFLTQLMQKEQRNYQFDFLRPQHSLFNYFTK
  LVEQYTKILIPPKGLFSKLKKEAENPREVLDQVCYRVEWAKFQERERKKEEEEKEKERVAYAQIDWHDFV
  VVETVDFQPNEQGNFPPPTTPEELGARILIQERYEKFGESEEVEMEVESDEEDDKQEKAEEPPSQLDQDT
  QVQDMDEGSDDEEEGQKVPPPPETPMPPPLPPTPDQVIVRKDYDPKASKPLPPAPAPDEYLVSPITGEKI
  PASKMQEHMRIGLLDPRWLEQRDRSIREKQSDDEVYAPGLDIESSLKQLAERRTDIFGVEETAIGKKIGE
  EEIQKPEEKVTWDGHSGSMARTQQAAQANITLQEQIEAIHKAKGLVPEDDTKEKIGPSKPNEIPQQPPPP
  SSATNIPSSAPPITSVPRPPTMPPPVRTTVVSAVPVMPRPPMASVVRLPPGSVIAPMPPIIHAPRINVVP
  MPPSAPPIMAPRPPPMIVPTAFVPAPPVAPVPAPAPMPPVHPPPPMEDEPTSKKLKTEDSLMPEEEFLRR
  NKGPVSIKVQVPNMQDKTEWKLNGQVLVFTLPLTDQVSVIKVKIHEATGMPAGKQKLQYEGIFIKDSNSL
  AYYNMANGAVIHLALKERGGRKK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005877
• ORF Size: 2379 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005877.1, NM_005877.2, NM_005877.3, NM_005877.4, NM_005877.5, NP_005868.1
• RefSeq Size: 5159 bp
• RefSeq ORF: 2382 bp
• Locus ID: 10291
• Domains: Surp, UBQ
• Protein Families: Druggable Genome
• Protein Pathways: Spliceosome
• MW: 88.9 kDa
• Gene Summary: This gene encodes a subunit of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer is a component of the mature U2 small nuclear ribonucleoprotein particle (snRNP). U2 small nuclear ribonucleoproteins play a critical role in spliceosome assembly and pre-mRNA splicing. [provided by RefSeq, Aug 2014]