ARPC1B (NM_005720) Human Tagged ORF Clone

CAT#: RC201103

ARPC1B (Myc-DDK-tagged)-Human actin related protein 2/3 complex, subunit 1B, 41kDa (ARPC1B)

Product Images

Western blot validation of overexpression lysate (Cat# LY417112) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201103 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ARPC1B protein (Cat# TP301103). The protein was produced from HEK293T cells transfected with ARPC1B cDNA clone (Cat# RC201103) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ARPC1B
• Synonyms: ARC41; p40-ARC; p41-ARC; PLTEID
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201103 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTACCACAGCTTCCTGGTGGAGCCCATCAGCTGCCACGCCTGGAACAAGGACCGCACCCAGATTG
  CCATCTGCCCCAACAACCATGAGGTGCATATCTATGAAAAGAGCGGTGCCAAATGGACCAAGGTGCACGA
  GCTCAAGGAGCACAACGGGCAGGTGACAGGCATCGACTGGGCCCCCGAGAGTAACCGTATTGTGACCTGC
  GGCACAGACCGCAACGCCTACGTGTGGACGCTGAAGGGCCGCACATGGAAGCCCACGCTGGTCATCCTGC
  GGATCAACCGGGCTGCCCGCTGCGTGCGCTGGGCCCCCAACGAGAACAAGTTTGCTGTGGGCAGCGGCTC
  TCGTGTGATCTCCATCTGTTATTTCGAGCAGGAGAATGACTGGTGGGTTTGCAAGCACATCAAGAAGCCC
  ATCCGCTCCACCGTCCTCAGCCTGGACTGGCACCCCAACAATGTGCTGCTGGCTGCCGGCTCCTGTGACT
  TCAAGTGTCGGATCTTTTCAGCCTACATCAAGGAGGTGGAGGAACGGCCGGCACCCACCCCGTGGGGCTC
  CAAGATGCCCTTTGGGGAACTGATGTTCGAATCCAGCAGTAGCTGCGGCTGGGTACATGGCGTCTGTTTC
  TCAGCCAGCGGGAGCCGCGTGGCCTGGGTAAGCCACGACAGCACCGTCTGCCTGGCTGATGCCGACAAGA
  AGATGGCCGTCGCGACTCTGGCCTCTGAAACACTACCACTGCTGGCGCTGACCTTCATCACAGACAACAG
  CCTGGTGGCAGCGGGCCACGACTGCTTCCCGGTGCTGTTCACCTATGACGCCGCCGCGGGGATGCTGAGC
  TTCGGCGGGCGGCTGGACGTTCCTAAGCAGAGCTCGCAGCGTGGCTTGACGGCCCGCGAGCGCTTCCAGA
  ACCTGGACAAGAAGGCGAGCTCCGAGGGTGGCACGGCTGCGGGCGCGGGCCTAGACTCGCTGCACAAGAA
  CAGCGTCAGCCAGATCTCGGTGCTCAGCGGCGGCAAGGCCAAGTGCTCGCAGTTCTGCACCACTGGCATG
  GATGGCGGCATGAGTATCTGGGATGTGAAGAGCTTGGAGTCAGCCTTGAAGGACCTCAAGATCAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201103 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAYHSFLVEPISCHAWNKDRTQIAICPNNHEVHIYEKSGAKWTKVHELKEHNGQVTGIDWAPESNRIVTC
  GTDRNAYVWTLKGRTWKPTLVILRINRAARCVRWAPNENKFAVGSGSRVISICYFEQENDWWVCKHIKKP
  IRSTVLSLDWHPNNVLLAAGSCDFKCRIFSAYIKEVEERPAPTPWGSKMPFGELMFESSSSCGWVHGVCF
  SASGSRVAWVSHDSTVCLADADKKMAVATLASETLPLLALTFITDNSLVAAGHDCFPVLFTYDAAAGMLS
  FGGRLDVPKQSSQRGLTARERFQNLDKKASSEGGTAAGAGLDSLHKNSVSQISVLSGGKAKCSQFCTTGM
  DGGMSIWDVKSLESALKDLKIK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005720
• ORF Size: 1116 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005720.1, NM_005720.2, NM_005720.3, NP_005711.1
• RefSeq Size: 1551 bp
• RefSeq ORF: 1119 bp
• Locus ID: 10095
• Domains: WD40
• Protein Pathways: Fc gamma R-mediated phagocytosis, Pathogenic Escherichia coli infection, Regulation of actin cytoskeleton
• MW: 40.9 kDa
• Gene Summary: This gene encodes one of seven subunits of the human Arp2/3 protein complex. This subunit is a member of the SOP2 family of proteins and is most similar to the protein encoded by gene ARPC1A. The similarity between these two proteins suggests that they both may function as p41 subunit of the human Arp2/3 complex that has been implicated in the control of actin polymerization in cells. It is possible that the p41 subunit is involved in assembling and maintaining the structure of the Arp2/3 complex. Multiple versions of the p41 subunit may adapt the functions of the complex to different cell types or developmental stages. This protein also has a role in centrosomal homeostasis by being an activator and substrate of the Aurora A kinase. [provided by RefSeq, Mar 2011]