TIP49A (RUVBL1) (NM_003707) Human Tagged ORF Clone

CAT#: RC201170

RUVBL1 (Myc-DDK-tagged)-Human RuvB-like 1 (E. coli) (RUVBL1)

Product Images

Western blot validation of overexpression lysate (Cat# LY418487) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201170 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified RUVBL1 protein (Cat# TP301170). The protein was produced from HEK293T cells transfected with RUVBL1 cDNA clone (Cat# RC201170) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RUVBL1
• Synonyms: ECP-54; ECP54; INO80H; NMP 238; NMP238; PONTIN; Pontin52; RVB1; TIH1; TIP49; TIP49A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201170 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGATTGAGGAGGTGAAGAGCACTACGAAGACGCAGCGCATCGCCTCCCACAGCCACGTGAAAGGGC
  TGGGGCTGGACGAGAGCGGCTTGGCCAAGCAGGCGGCCTCAGGGCTTGTGGGCCAGGAGAACGCGCGAGA
  GGCATGTGGCGTCATAGTAGAATTAATCAAAAGCAAGAAAATGGCTGGAAGAGCTGTCTTGTTGGCAGGA
  CCTCCTGGAACTGGCAAGACAGCTCTGGCTCTGGCTATTGCTCAGGAGCTGGGTAGTAAGGTCCCCTTCT
  GCCCAATGGTGGGGAGTGAAGTTTACTCAACTGAGATCAAGAAGACAGAGGTGCTGATGGAGAACTTCCG
  CAGGGCCATTGGGCTGCGAATAAAGGAGACCAAGGAAGTTTATGAAGGTGAAGTCACAGAGCTAACTCCG
  TGTGAGACAGAGAATCCCATGGGAGGATATGGCAAAACCATTAGCCATGTGATCATAGGACTCAAAACAG
  CCAAAGGAACCAAACAGTTGAAACTGGACCCCAGCATTTTTGAAAGTTTGCAGAAAGAGCGAGTAGAAGC
  TGGAGATGTGATTTACATTGAAGCCAACAGTGGGGCCGTGAAGAGGCAGGGCAGGTGTGATACCTATGCC
  ACAGAATTCGACCTTGAAGCTGAAGAGTATGTCCCCTTGCCAAAAGGGGATGTGCACAAAAAGAAAGAAA
  TCATCCAAGATGTGACCTTGCATGACTTGGATGTGGCTAATGCGCGGCCCCAGGGGGGACAAGATATCCT
  GTCCATGATGGGCCAGCTAATGAAGCCAAAGAAGACAGAAATCACAGACAAACTTCGAGGGGAGATTAAT
  AAGGTGGTGAACAAGTACATCGACCAGGGCATTGCTGAGCTGGTCCCGGGTGTGCTGTTTGTTGATGAGG
  TCCACATGCTGGACATTGAGTGCTTCACCTACCTGCACCGCGCCCTGGAGTCTTCTATCGCTCCCATCGT
  CATCTTTGCATCCAACCGAGGCAACTGTGTCATCAGAGGCACTGAGGACATCACATCCCCTCACGGCATC
  CCTCTTGACCTTCTGGACCGAGTGATGATAATCCGGACCATGCTGTATACTCCACAGGAAATGAAACAGA
  TCATTAAAATCCGTGCCCAGACGGAAGGAATCAACATCAGTGAGGAGGCACTGAACCACCTGGGGGAGAT
  TGGCACCAAGACCACACTGAGGTACTCAGTGCAGCTGCTGACCCCGGCCAACTTGCTTGCTAAAATCAAC
  GGGAAGGACAGCATTGAGAAAGAGCATGTCGAAGAGATCAGTGAACTTTTCTATGATGCCAAGTCCTCCG
  CCAAAATCCTGGCTGACCAGCAGGATAAGTACATGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201170 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKIEEVKSTTKTQRIASHSHVKGLGLDESGLAKQAASGLVGQENAREACGVIVELIKSKKMAGRAVLLAG
  PPGTGKTALALAIAQELGSKVPFCPMVGSEVYSTEIKKTEVLMENFRRAIGLRIKETKEVYEGEVTELTP
  CETENPMGGYGKTISHVIIGLKTAKGTKQLKLDPSIFESLQKERVEAGDVIYIEANSGAVKRQGRCDTYA
  TEFDLEAEEYVPLPKGDVHKKKEIIQDVTLHDLDVANARPQGGQDILSMMGQLMKPKKTEITDKLRGEIN
  KVVNKYIDQGIAELVPGVLFVDEVHMLDIECFTYLHRALESSIAPIVIFASNRGNCVIRGTEDITSPHGI
  PLDLLDRVMIIRTMLYTPQEMKQIIKIRAQTEGINISEEALNHLGEIGTKTTLRYSVQLLTPANLLAKIN
  GKDSIEKEHVEEISELFYDAKSSAKILADQQDKYMK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003707
• ORF Size: 1368 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003707.1, NM_003707.2, NP_003698.1
• RefSeq Size: 1785 bp
• RefSeq ORF: 1371 bp
• Locus ID: 8607
• Domains: AAA
• Protein Families: Stem cell - Pluripotency, Transcription Factors
• Protein Pathways: Wnt signaling pathway
• MW: 50.2 kDa
• Gene Summary: This gene encodes a protein that has both DNA-dependent ATPase and DNA helicase activities and belongs to the ATPases associated with diverse cellular activities (AAA+) protein family. The encoded protein associates with several multisubunit transcriptional complexes and with protein complexes involved in both ATP-dependent remodeling and histone modification. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]