Pumilio 1 (PUM1) (NM_014676) Human Tagged ORF Clone
CAT#: RC201219
PUM1 (Myc-DDK-tagged)-Human pumilio homolog 1 (Drosophila) (PUM1), transcript variant 2
"NM_014676" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | PUM1 |
Synonyms | HSPUM; PUMH; PUMH1; PUML1; SCA47 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC201219 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCGTTGCATGTGTCTTGAAGAGAAAAGCAGTGCTTTGGCAGGACTCTTTCAGCCCCCACCTGAAAC ATCACCCTCAAGAACCAGCTAATCCCAACATGCCTGTTGTTTTGACATCTGGAACAGGGTCGCAAGCGCA GCCACAACCAGCTGCAAATCAGGCTCTTGCAGCTGGGACTCACTCCAGCCCTGTCCCAGGATCTATAGGA GTTGCAGGCCGTTCCCAGGACGACGCTATGGTGGACTACTTCTTTCAGAGGCAGCATGGTGAGCAGCTTG GGGGAGGAGGAAGTGGAGGAGGCGGCTATAATAATAGCAAACATCGATGGCCTACTGGGGATAACATTCA TGCAGAACATCAGGTGCGTTCCATGGATGAACTGAATCATGATTTTCAAGCACTTGCTCTGGAGGGAAGA GCGATGGGAGAGCAGCTCTTGCCAGGTAAAAAGTTTTGGGAAACAGATGAATCCAGCAAAGATGGACCAA AAGGAATATTCCTGGGTGATCAATGGCGAGACAGTGCCTGGGGAACATCAGATCATTCAGTTTCCCAGCC AATCATGGTGCAGAGAAGACCTGGTCAGAGTTTCCATGTGAACAGTGAGGTCAATTCTGTACTGTCCCCA CGATCGGAGAGTGGGGGACTAGGCGTTAGCATGGTGGAGTATGTGTTGAGCTCATCCCCGGGCGATTCCT GTCTAAGAAAAGGAGGATTTGGCCCAAGGGATGCAGACAGTGATGAAAACGACAAAGGTGAAAAGAAGAA CAAGGGTACGTTTGATGGAGATAAGCTAGGAGATTTGAAGGAGGAGGGTGATGTGATGGACAAGACCAAT GGTTTACCAGTGCAGAATGGGATTGATGCAGACGTCAAAGATTTTAGCCGTACCCCTGGTAATTGCCAGA ACTCTGCTAATGAAGTGGATCTTCTGGGTCCAAACCAGAATGGTTCTGAGGGCTTAGCCCAGCTGACCAG CACCAATGGTGCCAAGCCTGTGGAGGATTTCTCCAACATGGAGTCCCAGAGTGTCCCCTTGGACCCCATG GAACATGTGGGCATGGAGCCTCTTCAGTTTGATTATTCAGGCACGCAGGTACCTGTGGACTCAGCAGCAG CAACTGTGGGACTTTTTGACTACAATTCTCAACAACAGCTGTTCCAAAGACCTAATGCGCTTGCTGTCCA GCAGTTGACAGCTGCTCAGCAGCAGCAGTATGCACTGGCAGCTGCTCATCAGCCGCACATCGGTTTAGCT CCCGCTGCGTTTGTCCCCAATCCATACATCATCAGCGCTGCTCCCCCAGGGACGGACCCCTACACAGCTG GATTGGCTGCAGCAGCGACACTAGGCCCAGCTGTGGTCCCTCACCAGTATTATGGAGTTACTCCCTGGGG AGTCTACCCTGCCAGTCTTTTCCAGCAGCAAGCTGCCGCTGCCGCTGCAGCAACTAATTCAGCTAATCAA CAGACCACCCCACAGGCTCAGCAAGGACAGCAGCAGGTTCTCCGTGGAGGAGCCAGCCAACGTCCTTTGA CCCCAAACCAGAACCAGCAGGGACAGCAAACGGATCCCCTTGTGGCAGCTGCAGCAGTGAATTCTGCCCT TGCATTTGGACAAGGTCTGGCAGCAGGCATGCCAGGTTATCCGGTGTTGGCTCCTGCTGCTTACTATGAC CAAACTGGTGCCCTTGTAGTGAATGCAGGCGCGAGAAATGGTCTTGGAGCTCCTGTTCGACTTGTAGCTC CTGCCCCAGTCATCATTAGTTCCTCAGCTGCACAAGCAGCTGTTGCAGCAGCCGCAGCTTCAGCAAATGG AGCAGCTGGTGGTCTTGCTGGAACAACAAATGGACCATTTCGCCCTTTAGGAACACAGCAGCCTCAGCCC CAGCCCCAGCAGCAGCCCAATAACAACCTGGCATCCAGTTCTTTCTACGGCAACAACTCTCTGAACAGCA ATTCACAGAGCAGCTCCCTCTTCTCCCAGGGCTCTGCCCAGCCTGCCAACACATCCTTGGGATTCGGAAG TAGCAGTTCTCTCGGCGCCACCCTGGGATCCGCCCTTGGAGGGTTTGGAACAGCAGTTGCAAACTCCAAC ACTGGCAGTGGCTCCCGCCGTGACTCCCTGACTGGCAGCAGTGACCTTTATAAGAGGACATCGAGCAGCT TGACCCCCATTGGACACAGTTTTTATAACGGCCTTAGCTTTTCCTCCTCTCCTGGACCCGTGGGCATGCC TCTCCCTAGTCAGGGACCAGGACATTCACAGACACCACCTCCTTCCCTCTCTTCACATGGATCCTCTTCA AGCTTAAACCTGGGAGGACTCACGAACGGCAGTGGAAGATACATCTCTGCTGCTCCAGGCGCTGAAGCCA AGTACCGCAGTGCAAGCAGCGCCTCCAGCCTCTTCAGCCCGAGCAGCACTCTTTTCTCTTCCTCTCGTTT GCGATATGGAATGTCTGATGTCATGCCTTCTGGCAGGAGCAGGCTTTTGGAAGATTTTCGAAACAACCGG TACCCCAATTTACAACTGCGGGAGATTGCTGGACATATAATGGAATTTTCCCAAGACCAGCATGGGTCCA GATTCATTCAGCTGAAACTGGAGCGTGCCACACCAGCTGAGCGCCAGCTTGTCTTCAATGAAATCCTCCA GGCTGCCTACCAACTCATGGTGGATGTGTTTGGTAATTACGTCATTCAGAAGTTCTTTGAATTTGGCAGT CTTGAACAGAAGCTGGCTTTGGCAGAACGGATTCGAGGCCACGTCCTGTCATTGGCACTACAGATGTATG GCTGCCGTGTTATCCAGAAAGCTCTTGAGTTTATTCCTTCAGACCAGCAGAATGAGATGGTTCGGGAACT AGATGGCCATGTCTTGAAGTGTGTGAAAGATCAGAATGGCAATCACGTGGTTCAGAAATGCATTGAATGT GTACAGCCCCAGTCTTTGCAATTTATCATCGATGCGTTTAAGGGACAGGTATTTGCCTTATCCACACATC CTTATGGCTGCCGAGTGATTCAGAGAATCCTGGAGCACTGTCTCCCTGACCAGACACTCCCTATTTTAGA GGAGCTTCACCAGCACACAGAGCAGCTTGTACAGGATCAATATGGAAATTATGTAATCCAACATGTACTG GAGCACGGTCGTCCTGAGGATAAAAGCAAAATTGTAGCAGAAATCCGAGGCAATGTACTTGTATTGAGTC AGCACAAATTTGCAAGCAATGTTGTGGAGAAGTGTGTTACTCACGCCTCACGTACGGAGCGCGCTGTGCT CATCGATGAGGTGTGCACCATGAACGACGGTCCCCACAGTGCCTTATACACCATGATGAAGGACCAGTAT GCCAACTACGTGGTCCAGAAGATGATTGACGTGGCGGAGCCAGGCCAGCGGAAGATCGTCATGCATAAGA TCCGGCCCCACATCGCAACTCTTCGTAAGTACACCTATGGCAAGCACATTCTGGCCAAGCTGGAGAAGTA CTACATGAAGAACGGTGTTGACTTAGGGCCCATCTGTGGCCCCCCTAATGGTATCATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC201219 protein sequence
Red=Cloning site Green=Tags(s) MSVACVLKRKAVLWQDSFSPHLKHHPQEPANPNMPVVLTSGTGSQAQPQPAANQALAAGTHSSPVPGSIG VAGRSQDDAMVDYFFQRQHGEQLGGGGSGGGGYNNSKHRWPTGDNIHAEHQVRSMDELNHDFQALALEGR AMGEQLLPGKKFWETDESSKDGPKGIFLGDQWRDSAWGTSDHSVSQPIMVQRRPGQSFHVNSEVNSVLSP RSESGGLGVSMVEYVLSSSPGDSCLRKGGFGPRDADSDENDKGEKKNKGTFDGDKLGDLKEEGDVMDKTN GLPVQNGIDADVKDFSRTPGNCQNSANEVDLLGPNQNGSEGLAQLTSTNGAKPVEDFSNMESQSVPLDPM EHVGMEPLQFDYSGTQVPVDSAAATVGLFDYNSQQQLFQRPNALAVQQLTAAQQQQYALAAAHQPHIGLA PAAFVPNPYIISAAPPGTDPYTAGLAAAATLGPAVVPHQYYGVTPWGVYPASLFQQQAAAAAAATNSANQ QTTPQAQQGQQQVLRGGASQRPLTPNQNQQGQQTDPLVAAAAVNSALAFGQGLAAGMPGYPVLAPAAYYD QTGALVVNAGARNGLGAPVRLVAPAPVIISSSAAQAAVAAAAASANGAAGGLAGTTNGPFRPLGTQQPQP QPQQQPNNNLASSSFYGNNSLNSNSQSSSLFSQGSAQPANTSLGFGSSSSLGATLGSALGGFGTAVANSN TGSGSRRDSLTGSSDLYKRTSSSLTPIGHSFYNGLSFSSSPGPVGMPLPSQGPGHSQTPPPSLSSHGSSS SLNLGGLTNGSGRYISAAPGAEAKYRSASSASSLFSPSSTLFSSSRLRYGMSDVMPSGRSRLLEDFRNNR YPNLQLREIAGHIMEFSQDQHGSRFIQLKLERATPAERQLVFNEILQAAYQLMVDVFGNYVIQKFFEFGS LEQKLALAERIRGHVLSLALQMYGCRVIQKALEFIPSDQQNEMVRELDGHVLKCVKDQNGNHVVQKCIEC VQPQSLQFIIDAFKGQVFALSTHPYGCRVIQRILEHCLPDQTLPILEELHQHTEQLVQDQYGNYVIQHVL EHGRPEDKSKIVAEIRGNVLVLSQHKFASNVVEKCVTHASRTERAVLIDEVCTMNDGPHSALYTMMKDQY ANYVVQKMIDVAEPGQRKIVMHKIRPHIATLRKYTYGKHILAKLEKYYMKNGVDLGPICGPPNGII TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_014676 |
ORF Size | 3558 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_014676.1, NM_014676.2, NP_055491.1 |
RefSeq Size | 5410 bp |
RefSeq ORF | 3561 bp |
Locus ID | 9698 |
Domains | PUF |
MW | 126.5 kDa |
Gene Summary | This gene encodes a member of the PUF family, evolutionarily conserved RNA-binding proteins related to the Pumilio proteins of Drosophila and the fem-3 mRNA binding factor proteins of C. elegans. The encoded protein contains a sequence-specific RNA binding domain comprised of eight repeats and N- and C-terminal flanking regions, and serves as a translational regulator of specific mRNAs by binding to their 3' untranslated regions. The evolutionarily conserved function of the encoded protein in invertebrates and lower vertebrates suggests that the human protein may be involved in translational regulation of embryogenesis, and cell development and differentiation. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC112626 | PUM1 (untagged)-Human pumilio homolog 1 (Drosophila) (PUM1), transcript variant 2 |
USD 310.00 |
|
RG201219 | PUM1 (GFP-tagged) - Human pumilio homolog 1 (Drosophila) (PUM1), transcript variant 2 |
USD 1,180.00 |
|
RC201219L1 | Lenti ORF clone of Human pumilio homolog 1 (Drosophila) (PUM1), transcript variant 2, Myc-DDK-tagged |
USD 1,572.00 |
|
RC201219L2 | Lenti ORF clone of Human pumilio homolog 1 (Drosophila) (PUM1), transcript variant 2, mGFP tagged |
USD 1,270.00 |
|
RC201219L3 | Lenti ORF clone of Human pumilio homolog 1 (Drosophila) (PUM1), transcript variant 2, Myc-DDK-tagged |
USD 1,572.00 |
|
RC201219L4 | Lenti ORF clone of Human pumilio homolog 1 (Drosophila) (PUM1), transcript variant 2, mGFP tagged |
USD 1,572.00 |
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