PRPF3 (NM_004698) Human Tagged ORF Clone

CAT#: RC201365

PRPF3 (Myc-DDK-tagged)-Human PRP3 pre-mRNA processing factor 3 homolog (S. cerevisiae) (PRPF3)

Product Images

Western blot validation of overexpression lysate (Cat# LY417826) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201365 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PRPF3
• Synonyms: HPRP3; HPRP3P; PRP3; Prp3p; RP18; SNRNP90
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201365 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCACTGTCAAAGAGGGAGCTGGATGAGCTGAAACCATGGATAGAGAAGACAGTGAAGAGGGTCCTGG
  GTTTCTCAGAGCCTACGGTGGTCACAGCAGCATTGAACTGTGTGGGGAAGGGCATGGACAAGAAGAAGGC
  AGCCGATCATCTGAAACCTTTTCTTGATGATTCTACTCTCCGATTTGTGGACAAACTGTTTGAGGCTGTG
  GAGGAAGGCCGAAGCTCTAGGCATTCCAAGTCTAGCAGTGACAGGAGCAGAAAACGAGAGCTAAAGGAGG
  TGTTTGGTGATGACTCTGAGATCTCTAAAGAATCATCAGGAGTAAAGAAGCGACGAATACCCCGTTTTGA
  GGAGGTGGAAGAAGAGCCAGAGGTGATCCCTGGGCCTCCATCAGAGAGCCCTGGCATGCTGACTAAGCTC
  CAGATCAAACAGATGATGGAGGCAGCAACACGACAAATCGAGGAGAGGAAAAAACAGCTGAGCTTCATTA
  GCCCCCCTACACCTCAGCCAAAGACTCCTTCTTCCTCCCAACCAGAACGACTTCCTATTGGCAACACTAT
  TCAGCCCTCCCAGGCTGCCACTTTCATGAATGATGCCATTGAGAAGGCAAGGAAAGCAGCTGAACTGCAA
  GCTCGAATCCAAGCCCAGCTGGCACTGAAGCCAGGACTCATCGGCAATGCCAACATGGTGGGCCTGGCTA
  ATCTCCATGCCATGGGCATTGCTCCCCCGAAGGTGGAGTTAAAAGACCAAACGAAACCTACACCACTGAT
  CCTGGATGAGCAAGGGCGCACTGTAGATGCAACAGGCAAGGAGATTGAGCTGACACACCGCATGCCTACT
  CTGAAAGCCAATATTCGTGCTGTGAAGAGGGAACAATTCAAGCAACAACTAAAGGAAAAGCCATCAGAAG
  ACATGGAATCCAATACCTTTTTTGACCCCCGAGTCTCCATTGCCCCTTCCCAGCGCCAGAGACGCACTTT
  TAAATTCCATGACAAGGGCAAATTTGAGAAGATTGCTCAGCGATTACGGACAAAGGCTCAACTGGAGAAG
  CTACAGGCAGAGATTTCACAAGCAGCTCGAAAAACAGGCATCCATACTTCGACTAGGCTTGCCCTCATTG
  CTCCTAAGAAGGAGCTAAAGGAAGGAGATATTCCTGAAATTGAGTGGTGGGACTCTTACATAATCCCCAA
  TGGCTTTGATCTTACAGAGGAAAATCCCAAGAGAGAAGATTATTTTGGAATCACAAATCTTGTTGAACAT
  CCAGCCCAGCTCAATCCTCCAGTTGACAATGACACACCAGTTACTCTGGGAGTATATCTTACCAAGAAGG
  AACAGAAAAAACTTCGGAGACAAACAAGGAGGGAAGCACAGAAGGAACTACAAGAAAAAGTCAGGCTGGG
  CCTGATGCCTCCTCCAGAACCCAAAGTGAGAATTTCTAATTTGATGCGAGTATTAGGAACAGAAGCTGTT
  CAAGACCCCACGAAGGTAGAAGCCCACGTCAGAGCTCAGATGGCAAAAAGACAGAAAGCGCATGAAGAGG
  CCAACGCTGCCCGAAAACTCACAGCAGAACAGAGAAAGGTCAAGAAAATTAAAAAGCTTAAAGAAGACAT
  TTCACAGGGGGTACACATATCTGTATATAGAGTTCGAAATTTGAGCAACCCAGCCAAGAAGTTCAAGATT
  GAAGCCAATGCTGGGCAACTGTACCTGACAGGGGTGGTGGTACTGCACAAGGATGTCAACGTGGTAGTAG
  TGGAAGGGGGCCCCAAGGCCCAGAAGAAATTTAAGCGTCTTATGCTGCATCGGATAAAGTGGGATGAACA
  GACATCTAACACAAAGGGAGATGATGATGAGGAGTCTGATGAGGAAGCTGTGAAGAAAACCAACAAATGT
  GTACTAGTCTGGGAGGGTACAGCCAAAGACCGGAGCTTTGGAGAGATGAAGTTTAAACAGTGTCCTACAG
  AGAACATGGCTCGTGAGCATTTCAAAAAGCATGGGGCTGAACACTACTGGGACCTTGCGCTGAGTGAATC
  TGTGTTAGAGTCCACTGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201365 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MALSKRELDELKPWIEKTVKRVLGFSEPTVVTAALNCVGKGMDKKKAADHLKPFLDDSTLRFVDKLFEAV
  EEGRSSRHSKSSSDRSRKRELKEVFGDDSEISKESSGVKKRRIPRFEEVEEEPEVIPGPPSESPGMLTKL
  QIKQMMEAATRQIEERKKQLSFISPPTPQPKTPSSSQPERLPIGNTIQPSQAATFMNDAIEKARKAAELQ
  ARIQAQLALKPGLIGNANMVGLANLHAMGIAPPKVELKDQTKPTPLILDEQGRTVDATGKEIELTHRMPT
  LKANIRAVKREQFKQQLKEKPSEDMESNTFFDPRVSIAPSQRQRRTFKFHDKGKFEKIAQRLRTKAQLEK
  LQAEISQAARKTGIHTSTRLALIAPKKELKEGDIPEIEWWDSYIIPNGFDLTEENPKREDYFGITNLVEH
  PAQLNPPVDNDTPVTLGVYLTKKEQKKLRRQTRREAQKELQEKVRLGLMPPPEPKVRISNLMRVLGTEAV
  QDPTKVEAHVRAQMAKRQKAHEEANAARKLTAEQRKVKKIKKLKEDISQGVHISVYRVRNLSNPAKKFKI
  EANAGQLYLTGVVVLHKDVNVVVVEGGPKAQKKFKRLMLHRIKWDEQTSNTKGDDDEESDEEAVKKTNKC
  VLVWEGTAKDRSFGEMKFKQCPTENMAREHFKKHGAEHYWDLALSESVLESTD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004698
• ORF Size: 2049 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004698.1, NM_004698.2, NP_004689.1
• RefSeq Size: 2480 bp
• RefSeq ORF: 2052 bp
• Locus ID: 9129
• Domains: PWI
• Protein Pathways: Spliceosome
• MW: 77.5 kDa
• Gene Summary: The removal of introns from nuclear pre-mRNAs occurs on complexes called spliceosomes, which are made up of 4 small nuclear ribonucleoprotein (snRNP) particles and an undefined number of transiently associated splicing factors. This gene product is one of several proteins that associate with U4 and U6 snRNPs. Mutations in this gene are associated with retinitis pigmentosa-18. [provided by RefSeq, Jul 2008]