BMERB1 (NM_033201) Human Tagged ORF Clone

CAT#: RC201398

C16orf45 (Myc-DDK-tagged)-Human chromosome 16 open reading frame 45 (C16orf45), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY409626) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201398 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified BMERB1 protein (Cat# TP301398). The protein was produced from HEK293T cells transfected with BMERB1 cDNA clone (Cat# RC201398) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BMERB1
• Synonyms: C16orf45; MINP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201398 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAATTAAAGCAATCTTTGTCCACCCATCTGGAAGCCGAGAAGCCTCTGAGGCGCTATGGGGCGGTGG
  AGGAGACGGCTTGGAAAACGGAGAGACTGGGGAGAAATCAGCTGGACATCATCTCCATGGCGGAGACAAC
  CATGATGCCAGAGGAGATTGAGCTGGAGATGGCAAAAATTCAGCGTCTCCGGGAAGTCTTGGTCCGCCGG
  GAGTCTGAGCTCAGGTTCATGATGGATGACATCCAGCTCTGCAAGGACATCATGGACTTGAAGCAGGAGC
  TGCAGAACTTGGTCGCCATCCCAGAAAAAGAAAAAACCAAACTGCAGAAGCAGAGAGAGGATGAGCTAAT
  CCAGAAGATCCACAAACTGGTGCAGAAGAGAGACTTCCTGGTGGACGATGCGGAGGTCGAGCGGTTAAGG
  GAGCAAGAAGAAGACAAGGAAATGGCTGATTTCCTGAGAATCAAGTTAAAACCTCTAGACAAAGTAACCA
  AATCTCCAGCCAGCTCCCGGGCAGAGAAGAAAGCAGAGCCCCCACCTAGCAAGCCCACGGTGGCCAAGAC
  GGGGCTGGCACTGATCAAGGATTGTTGCGGGGCCACCCAGTGCAACATCATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201398 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MELKQSLSTHLEAEKPLRRYGAVEETAWKTERLGRNQLDIISMAETTMMPEEIELEMAKIQRLREVLVRR
  ESELRFMMDDIQLCKDIMDLKQELQNLVAIPEKEKTKLQKQREDELIQKIHKLVQKRDFLVDDAEVERLR
  EQEEDKEMADFLRIKLKPLDKVTKSPASSRAEKKAEPPPSKPTVAKTGLALIKDCCGATQCNIM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_033201
• ORF Size: 612 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033201.1, NM_033201.2, NP_149978.1
• RefSeq Size: 2234 bp
• RefSeq ORF: 615 bp
• Locus ID: 89927
• MW: 23.7 kDa