ABCF3 (NM_018358) Human Tagged ORF Clone

CAT#: RC201403

ABCF3 (Myc-DDK-tagged)-Human ATP-binding cassette, sub-family F (GCN20), member 3 (ABCF3)

Product Images

Western blot validation of overexpression lysate (Cat# LY413110) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201403 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified ABCF3 protein (Cat# TP301403). The protein was produced from HEK293T cells transfected with ABCF3 cDNA clone (Cat# RC201403) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ABCF3
• Synonyms: EST201864
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201403 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGACTTGCGCCGAAATCCTGCGGAGCGAGTTCCCCGAAATTGACGGACAAGTCTTCGACTACGTGA
  CCGGCGTCTTGCACAGCGGCAGCGCGGACTTCGAGTCTGTGGATGACCTGGTGGAAGCTGTAGGGGAACT
  ATTGCAAGAGGTGTCCGGGGACAGCAAGGATGACGCGGGCATCAGGGCCGTGTGCCAGCGCATGTACAAC
  ACTCTGCGTCTGGCTGAGCCACAAAGCCAGGGAAATAGCCAGGTGCTACTGGACGCCCCTATCCAGTTGT
  CAAAGATAACGGAGAACTACGACTGTGGAACCAAACTTCCAGGACTGCTAAAGAGGGAACAGTCCTCGAC
  AGTGAATGCAAAGAAGTTAGAGAAGGCCGAGGCTCGACTTAAGGCAAAGCAGGAGAAGCGCTCAGAGAAG
  GACACGCTCAAGACCAGCAACCCTCTAGTCTTAGAAGAGGCATCAGCCAGCCAGGCAGGCAGCAGAAAGG
  AGAGTCGGTTGGAATCATCTGGCAAGAACAAATCCTATGATGTGCGAATTGAGAACTTTGATGTGTCTTT
  TGGCGATAGAGTACTGCTGGCTGGAGCGGATGTGAACCTGGCATGGGGCCGCCGTTACGGGCTGGTGGGG
  CGGAATGGGTTGGGGAAGACAACGTTACTGAAGATGCTGGCCACCCGGAGTCTGCGGGTTCCAGCCCACA
  TTTCCCTGCTGCACGTTGAGCAAGAGGTTGCTGGAGATGACACTCCTGCCCTGCAGAGTGTGCTGGAGAG
  TGACAGTGTGCGAGAGGATTTGTTACGGAGGGAGCGGGAGCTCACTGCCCAGATTGCTGCTGGCAGGGCG
  GAGGGCTCTGAAGCTGCAGAGCTGGCAGAAATCTATGCCAAACTGGAGGAGATTGAGGCTGACAAGGCAC
  CTGCCAGGGCATCAGTCATTCTCGCTGGGCTTGGCTTTACCCCTAAAATGCAGCAGCAGCCCACCCGGGA
  GTTCTCAGGTGGCTGGAGGATGAGGCTGGCCCTGGCCCGGGCCCTCTTTGCTAGGCCAGATCTTCTGCTG
  TTAGATGAACCTACAAACATGCTGGATGTCAGGGCCATCCTGTGGCTGGAGAATTACCTGCAGACGTGGC
  CCTCCACCATCCTAGTCGTCTCCCACGACCGCAACTTCTTGAATGCCATCGCCACAGACATCATCCACCT
  GCACAGCCAGCGGCTAGATGGTTACCGGGGAGACTTTGAGACCTTCATCAAGAGTAAGCAGGAGCGGCTG
  CTCAACCAGCAGCGTGAATATGAGGCGCAGCAGCAGTATCGCCAGCACATCCAGGTTTTCATTGACCGGT
  TTCGCTACAATGCCAACAGGGCCTCTCAAGTGCAGAGTAAACTCAAGATGCTGGAGAAGCTGCCTGAGCT
  GAAGCCTGTGGACAAGGAATCAGAGGTCGTAATGAAGTTCCCTGATGGGTTTGAGAAGTTCTCGCCGCCA
  ATTCTGCAGCTAGATGAGGTGGATTTCTACTACGATCCGAAGCACGTCATCTTCAGTCGCCTCTCTGTGT
  CTGCTGATCTCGAGTCTCGCATCTGTGTGGTTGGAGAGAATGGGGCTGGGAAGTCTACCATGCTGAAGCT
  GCTTTTGGGGGACCTGGCACCTGTTCGGGGCATCAGACACGCTCACAGGAATCTGAAGATTGGCTATTTC
  AGCCAGCACCATGTGGAGCAGCTGGACCTAAACGTCAGTGCTGTGGAACTGCTGGCACGCAAGTTTCCTG
  GGCGGCCTGAGGAGGAGTACCGTCACCAGCTGGGTCGGTATGGCATCTCCGGAGAACTGGCCATGCGTCC
  TCTTGCCAGCCTGTCTGGGGGCCAGAAGAGCCGAGTGGCCTTTGCTCAGATGACTATGCCCTGCCCCAAC
  TTCTACATTCTGGATGAACCCACAAACCACCTGGACATGGAGACCATTGAGGCTCTGGGCCGTGCCCTCA
  ACAATTTCAGGGGTGGTGTGATTCTGGTGTCCCACGATGAGCGCTTTATCAGGCTGGTGTGCCGGGAGTT
  GTGGGTATGCGAAGGAGGCGGCGTCACCCGTGTGGAAGGAGGATTTGACCAGTACCGCGCCCTCCTCCAG
  GAACAGTTCCGCCGCGAAGGCTTCCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201403 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MATCAEILRSEFPEIDGQVFDYVTGVLHSGSADFESVDDLVEAVGELLQEVSGDSKDDAGIRAVCQRMYN
  TLRLAEPQSQGNSQVLLDAPIQLSKITENYDCGTKLPGLLKREQSSTVNAKKLEKAEARLKAKQEKRSEK
  DTLKTSNPLVLEEASASQAGSRKESRLESSGKNKSYDVRIENFDVSFGDRVLLAGADVNLAWGRRYGLVG
  RNGLGKTTLLKMLATRSLRVPAHISLLHVEQEVAGDDTPALQSVLESDSVREDLLRRERELTAQIAAGRA
  EGSEAAELAEIYAKLEEIEADKAPARASVILAGLGFTPKMQQQPTREFSGGWRMRLALARALFARPDLLL
  LDEPTNMLDVRAILWLENYLQTWPSTILVVSHDRNFLNAIATDIIHLHSQRLDGYRGDFETFIKSKQERL
  LNQQREYEAQQQYRQHIQVFIDRFRYNANRASQVQSKLKMLEKLPELKPVDKESEVVMKFPDGFEKFSPP
  ILQLDEVDFYYDPKHVIFSRLSVSADLESRICVVGENGAGKSTMLKLLLGDLAPVRGIRHAHRNLKIGYF
  SQHHVEQLDLNVSAVELLARKFPGRPEEEYRHQLGRYGISGELAMRPLASLSGGQKSRVAFAQMTMPCPN
  FYILDEPTNHLDMETIEALGRALNNFRGGVILVSHDERFIRLVCRELWVCEGGGVTRVEGGFDQYRALLQ
  EQFRREGFL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018358
• ORF Size: 2127 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018358.1, NM_018358.2, NP_060828.1
• RefSeq Size: 2626 bp
• RefSeq ORF: 2130 bp
• Locus ID: 55324
• Domains: ABC_tran, AAA
• Protein Families: Druggable Genome
• MW: 79.7 kDa
• Gene Summary: This gene encodes a member of the ATP-binding cassette (ABC) transporter superfamily. ATP-binding cassette proteins transport various molecules across extra- and intracellular membranes. The protein encoded by this gene displays antiviral effect against flaviviruses. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2017]