FZR1 (NM_016263) Human Tagged ORF Clone

CAT#: RC201413

FZR1 (Myc-DDK-tagged)-Human fizzy/cell division cycle 20 related 1 (Drosophila) (FZR1), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY402526) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201413 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified FZR1 protein (Cat# TP301413). The protein was produced from HEK293T cells transfected with FZR1 cDNA clone (Cat# RC201413) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FZR1
• Synonyms: CDC20C; CDH1; FZR; FZR2; HCDH; HCDH1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201413 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCAGGACTATGAGCGGCGCCTGCTTCGCCAGATCGTCATCCAGAATGAGAACACGATGCCACGCG
  TCACAGAGATGCGGCGGACCCTGACGCCTGCCAGCTCCCCAGTGTCCTCGCCCAGCAAGCACGGAGACCG
  CTTCATCCCCTCCAGAGCCGGAGCCAACTGGAGCGTGAACTTCCACAGGATTAACGAGAATGAGAAGTCT
  CCCAGTCAGAACCGGAAAGCCAAGGACGCCACCTCAGACAACGGCAAAGACGGCCTGGCCTACTCTGCCC
  TGCTCAAGAATGAGCTGCTGGGTGCCGGCATCGAGAAGGTGCAGGACCCGCAGACTGAGGACCGCAGGCT
  GCAGCCCTCCACGCCTGAGAAGAAGGGTCTGTTCACGTATTCCCTTAGCACCAAGCGCTCCAGCCCCGAT
  GACGGCAACGATGTGTCTCCCTACTCCCTGTCTCCCGTCAGCAACAAGAGCCAGAAGCTGCTCCGGTCCC
  CCCGGAAACCCACCCGCAAGATCTCCAAGATCCCCTTCAAGGTGCTGGACGCGCCCGAGCTGCAGGACGA
  CTTCTACCTCAATCTGGTGGACTGGTCGTCCCTCAATGTGCTCAGCGTGGGGCTAGGCACCTGCGTGTAC
  CTGTGGAGTGCCTGTACCAGCCAGGTGACGCGGCTCTGTGACCTCTCAGTGGAAGGGGACTCAGTGACCT
  CCGTGGGCTGGTCTGAGCGGGGGAACCTGGTGGCGGTGGGCACACACAAGGGCTTCGTGCAGATCTGGGA
  CGCAGCCGCAGGGAAGAAGCTGTCCATGTTGGAGGGCCACACGGCACGCGTCGGGGCGCTGGCCTGGAAT
  GCTGAGCAGCTGTCGTCCGGGAGCCGCGACCGCATGATCCTGCAGAGGGACATCCGCACCCCGCCACTGC
  AGTCGGAGCGGCGGCTGCAGGGCCACCGGCAGGAGGTGTGCGGGCTCAAGTGGTCCACAGACCACCAGCT
  CCTCGCCTCGGGGGGCAACGACAACAAGCTGCTGGTCTGGAATCACTCGAGCCTGAGCCCCGTGCAGCAG
  TACACGGAGCACCTGGCGGCCGTGAAGGCCATCGCCTGGTCCCCACATCAGCACGGGCTGCTGGCCTCGG
  GGGGCGGCACAGCTGACCGCTGTATCCGCTTCTGGAACACGCTGACAGGACAACCACTGCAGTGTATCGA
  CACGGGCTCCCAAGTGTGCAATCTGGCCTGGTCCAAGCACGCCAACGAGCTGGTGAGCACGCACGGCTAC
  TCACAGAACCAGATCCTTGTCTGGAAGTACCCCTCCCTGACCCAGGTGGCCAAGCTGACCGGGCACTCCT
  ACCGCGTGCTGTACCTGGCAATGTCCCCTGATGGGGAGGCCATCGTCACTGGTGCTGGAGACGAGACCCT
  GAGGTTCTGGAACGTCTTTAGCAAAACCCGTTCGACAAAGGAGTCTGTGTCTGTGCTCAACCTCTTCACC
  AGGATCCGG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201413 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDQDYERRLLRQIVIQNENTMPRVTEMRRTLTPASSPVSSPSKHGDRFIPSRAGANWSVNFHRINENEKS
  PSQNRKAKDATSDNGKDGLAYSALLKNELLGAGIEKVQDPQTEDRRLQPSTPEKKGLFTYSLSTKRSSPD
  DGNDVSPYSLSPVSNKSQKLLRSPRKPTRKISKIPFKVLDAPELQDDFYLNLVDWSSLNVLSVGLGTCVY
  LWSACTSQVTRLCDLSVEGDSVTSVGWSERGNLVAVGTHKGFVQIWDAAAGKKLSMLEGHTARVGALAWN
  AEQLSSGSRDRMILQRDIRTPPLQSERRLQGHRQEVCGLKWSTDHQLLASGGNDNKLLVWNHSSLSPVQQ
  YTEHLAAVKAIAWSPHQHGLLASGGGTADRCIRFWNTLTGQPLQCIDTGSQVCNLAWSKHANELVSTHGY
  SQNQILVWKYPSLTQVAKLTGHSYRVLYLAMSPDGEAIVTGAGDETLRFWNVFSKTRSTKESVSVLNLFT
  RIR
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016263
• ORF Size: 1479 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016263.2, NM_016263.3, NP_057347.2
• RefSeq Size: 3615 bp
• RefSeq ORF: 1482 bp
• Locus ID: 51343
• Domains: WD40
• Protein Families: Druggable Genome
• Protein Pathways: Cell cycle, Progesterone-mediated oocyte maturation, Ubiquitin mediated proteolysis
• MW: 54.8 kDa