FAM110A (NM_207121) Human Tagged ORF Clone

CAT#: RC201431

FAM110A (Myc-DDK-tagged)-Human family with sequence similarity 110, member A (FAM110A), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY410535) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204584 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified FAM110A protein (Cat# TP301431). The protein was produced from HEK293T cells transfected with FAM110A cDNA clone (Cat# RC201431) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FAM110A
• Synonyms: bA371L19.3; C20orf55; F10
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201431 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTGTGCACACGCTGAGCCCCGGAGCCCCGTCCGCCCCCGCCCTACCTTGCCGCCTGCGGACCAGGG
  TCCCTGGCTACCTGCTACGGGGGCCGGCAGATGGTGGAGCCCGGAAACCGAGCGCTGTGGAGCGCCTGGA
  GGCCGACAAGGCCAAGTACGTCAAGAGCCTGCACGTGGCCAACACCCGCCAGGAGCCTGTGCAGCCCCTG
  CTGTCCAAACAGCCGCTCTTCAGCCCTGAGACTCGCCGCACAGTGCTCACGCCCAGCCGCCGAGCCCTGC
  CTGGCCCCTGCCGACGGCCCCAGCTGGACCTGGACATCCTCAGCAGCCTCATCGACTTGTGTGACAGCCC
  CGTGTCCCCTGCCGAGGCCAGCCGCACTCCTGGACGGGCCGAGGGAGCCGGCCGTCCTCCCCCAGCCACC
  CCTCCGCGACCGCCGCCCAGTACCTCTGCGGTCCGCCGGGTGGACGTCCGCCCCCTGCCCGCCTCGCCTG
  CCCGGCCCTGCCCATCACCCGGCCCTGCCGCCGCCTCCAGCCCAGCCCGGCCGCCGGGTTTGCAACGCTC
  CAAGTCGGACTTGAGCGAGCGCTTTTCTAGGGCAGCCGCTGATCTCGAGCGCTTTTTTAACTTCTGCGGC
  CTGGACCCGGAGGAGGCGAGAGGGTTGGGTGTGGCCCACCTGGCACGGGCCAGCTCGGATATCGTGTCCC
  TGGCAGGGCCCAGTGCTGGGCCGGGCAGCTCTGAAGGGGGCTGCTCCCGCCGCAGCTCGGTGACTGTTGA
  GGAGCGGGCCCGGGAGCGCGTTCCCTATGGCGTGTCGGTGGTGGAGCGCAATGCCCGCGTGATCAAGTGG
  TTGTATGGGCTAAGGCAGGCTCGGGAGAGCCCAGCAGCTGAAGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201431 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPVHTLSPGAPSAPALPCRLRTRVPGYLLRGPADGGARKPSAVERLEADKAKYVKSLHVANTRQEPVQPL
  LSKQPLFSPETRRTVLTPSRRALPGPCRRPQLDLDILSSLIDLCDSPVSPAEASRTPGRAEGAGRPPPAT
  PPRPPPSTSAVRRVDVRPLPASPARPCPSPGPAAASSPARPPGLQRSKSDLSERFSRAAADLERFFNFCG
  LDPEEARGLGVAHLARASSDIVSLAGPSAGPGSSEGGCSRRSSVTVEERARERVPYGVSVVERNARVIKW
  LYGLRQARESPAAEG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_207121
• ORF Size: 885 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_207121.1, NM_207121.2, NM_207121.3, NM_207121.4, NP_997004.1
• RefSeq Size: 1619 bp
• RefSeq ORF: 888 bp
• Locus ID: 83541
• MW: 31.3 kDa