Breast cancer suppressor candidate 1 (VWA5A) (NM_198315) Human Tagged ORF Clone

CAT#: RC201474

VWA5A (Myc-DDK-tagged)-Human von Willebrand factor A domain containing 5A (VWA5A), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY405013) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201474 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified VWA5A protein (Cat# TP301474). The protein was produced from HEK293T cells transfected with VWA5A cDNA clone (Cat# RC201474) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: VWA5A
• Synonyms: BCSC-1; BCSC1; LOH11CR2A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201474 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGCACTTCTGTGGCCTACTCACCCTCCACCGGGAGCCAGTGCCGCTGAAGAGTATCTCTGTGAGCG
  TGAACATTTACGAGTTTGTGGCTGGTGTGTCTGCAACTTTGAACTACGAGAATGAGGAGAAAGTTCCTTT
  GGAGGCCTTCTTTGTGTTCCCCATGGATGAAGACTCTGCTGTTTACAGCTTTGAGGCCTTGGTGGATGGG
  AAGAAAATTGTAGCAGAATTACAAGACAAGATGAAGGCCCGCACCAACTATGAGAAAGCCATCTCCCAGG
  GCCACCAGGCCTTCTTATTGGAGGGGGACAGCAGCTCCAGGGATGTCTTCTCTTGCAATGTGGGTAACCT
  CCAACCTGGGTCGAAGGCGGCAGTCACCCTGAAGTATGTGCAGGAGCTGCCTCTGGAAGCAGATGGGGCT
  CTGCGCTTTGTGCTCCCAGCTGTCCTGAATCCTAGATACCAGTTCTCTGGGTCGTCTAAGGACAGTTGCC
  TTAATGTGAAGACTCCTATAGTCCCTGTGGAGGACCTGCCCTACACACTCAGCATGGTCGCCACCATAGA
  TTCCCAGCATGGCATTGAGAAGGTCCAATCCAACTGCCCCTTGAGTCCTACCGAGTACCTAGGAGAGGAC
  AAGACTTCTGCTCAGGTTTCCCTGGCTGCTGGACACAAGTTTGATCGGGACGTGGAACTCCTGATTTACT
  ACAATGAGGTGCATACCCCCAGCGTGGTTTTGGAGATGGGGATGCCTAACATGAAGCCAGGTCATTTGAT
  GGGAGATCCATCTGCAATGGTGAGTTTCTATCCAAATATCCCAGAAGATCAACCATCAAATACCTGTGGA
  GAGTTTATCTTTCTCATGGACCGCTCGGGAAGTATGCAGAGCCCCATGAGTAGCCAGGATACATCTCAGC
  TGCGAATACAGGCAGCCAAGGAAACACTGATTTTGCTGCTGAAGAGTTTACCTATAGGCTGTTATTTCAA
  CATCTATGGATTTGGCTCTTCCTATGAGGCATGCTTTCCGGAGAGTGTGAAGTACACTCAGCAAACAATG
  GAGGAGGCTCTGGGGAGAGTGAAGCTTATGCAGGCCGACCTAGGGGGCACTGAAATCTTGGCACCACTCC
  AGAACATTTACAGGGGACCCTCCATCCCAGGCCACCCCCTACAGCTTTTTGTCTTTACAGATGGAGAAGT
  TACAGACACGTTTAGTGTAATTAAAGAAGTTAGGATCAACAGACAGAAACACAGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201474 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVHFCGLLTLHREPVPLKSISVSVNIYEFVAGVSATLNYENEEKVPLEAFFVFPMDEDSAVYSFEALVDG
  KKIVAELQDKMKARTNYEKAISQGHQAFLLEGDSSSRDVFSCNVGNLQPGSKAAVTLKYVQELPLEADGA
  LRFVLPAVLNPRYQFSGSSKDSCLNVKTPIVPVEDLPYTLSMVATIDSQHGIEKVQSNCPLSPTEYLGED
  KTSAQVSLAAGHKFDRDVELLIYYNEVHTPSVVLEMGMPNMKPGHLMGDPSAMVSFYPNIPEDQPSNTCG
  EFIFLMDRSGSMQSPMSSQDTSQLRIQAAKETLILLLKSLPIGCYFNIYGFGSSYEACFPESVKYTQQTM
  EEALGRVKLMQADLGGTEILAPLQNIYRGPSIPGHPLQLFVFTDGEVTDTFSVIKEVRINRQKHR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_198315
• ORF Size: 1245 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198315.1, NM_198315.2, NP_938057.1
• RefSeq Size: 1874 bp
• RefSeq ORF: 1248 bp
• Locus ID: 4013
• Cytogenetics: 11q24.2
• MW: 45.9 kDa
• Gene Summary: ''