ERGIC2 (NM_016570) Human Tagged ORF Clone

CAT#: RC201479

ERGIC2 (Myc-DDK-tagged)-Human ERGIC and golgi 2 (ERGIC2)

Product Images

Western blot validation of overexpression lysate (Cat# LY413893) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201479 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ERGIC2
• Synonyms: cd002; CDA14; Erv41; PTX1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201479 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGCGACTGAATCGGAAAAAAACTTTAAGTTTGGTAAAAGAGTTGGATGCCTTTCCGAAGGTTCCTG
  AGAGCTATGTAGAGACTTCAGCCAGTGGAGGTACAGTTTCTCTAATAGCATTTACAACTATGGCTTTATT
  AACCATAATGGAATTCTCAGTATATCAAGATACATGGATGAAGTATGAATACGAAGTAGACAAGGATTTT
  TCTAGCAAATTAAGAATTAATATAGATATTACTGTTGCCATGAAGTGTCAATATGTTGGAGCGGATGTAT
  TGGATTTAGCAGAAACAATGGTTGCATCTGCAGATGGTTTAGTTTATGAACCAACAGTATTTGATCTTTC
  ACCACAGCAGAAAGAGTGGCAGAGGATGCTGCAGCTGATTCAGAGTAGGCTACAAGAAGAGCATTCACTT
  CAAGATGTGATATTTAAAAGTGCTTTTAAAAGTACATCAACAGCTCTTCCACCAAGAGAAGATGATTCAT
  CACAGTCTCCAAATGCATGCAGAATTCATGGCCATCTATATGTCAATAAAGTAGCAGGGAATTTTCACAT
  AACAGTGGGCAAGGCAATTCCACATCCTCGTGGTCATGCACATTTGGCAGCACTTGTCAACCATGAATCT
  TACAATTTTTCTCATAGAATAGATCATTTGTCTTTTGGAGAGCTTGTTCCAGCAATTATTAATCCTTTAG
  ATGGAACTGAAAAAATTGCTATAGATCACAACCAGATGTTCCAATATTTTATTACAGTTGTGCCAACAAA
  ACTACATACATATAAAATATCAGCAGACACCCATCAGTTTTCTGTGACAGAAAGGGAACGTATCATTAAC
  CATGCTGCAGGCAGCCATGGAGTCTCTGGGATATTTATGAAATATGATCTCAGTTCTCTTATGGTGACAG
  TTACTGAGGAGCACATGCCATTCTGGCAGTTTTTTGTAAGACTCTGTGGTATTGTTGGAGGAATCTTTTC
  AACAACAGGCATGTTACATGGAATTGGAAAATTTATAGTTGAAATAATTTGCTGTCGTTTCAGACTTGGA
  TCCTATAAACCTGTCAATTCTGTTCCTTTTGAGGATGGCCACACAGACAACCACTTACCTCTTTTAGAAA
  ATAATACACAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201479 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRRLNRKKTLSLVKELDAFPKVPESYVETSASGGTVSLIAFTTMALLTIMEFSVYQDTWMKYEYEVDKDF
  SSKLRINIDITVAMKCQYVGADVLDLAETMVASADGLVYEPTVFDLSPQQKEWQRMLQLIQSRLQEEHSL
  QDVIFKSAFKSTSTALPPREDDSSQSPNACRIHGHLYVNKVAGNFHITVGKAIPHPRGHAHLAALVNHES
  YNFSHRIDHLSFGELVPAIINPLDGTEKIAIDHNQMFQYFITVVPTKLHTYKISADTHQFSVTERERIIN
  HAAGSHGVSGIFMKYDLSSLMVTVTEEHMPFWQFFVRLCGIVGGIFSTTGMLHGIGKFIVEIICCRFRLG
  SYKPVNSVPFEDGHTDNHLPLLENNTH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016570
• ORF Size: 1131 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016570.1, NM_016570.2, NP_057654.2
• RefSeq Size: 1781 bp
• RefSeq ORF: 1134 bp
• Locus ID: 51290
• MW: 42.5 kDa
• Gene Summary: ERGIC2, or PTX1, is a ubiquitously expressed nuclear protein that is downregulated in prostate carcinoma (Kwok et al., 2001 [PubMed 11445006]). [supplied by OMIM, Aug 2008]